Separation of thio- and oxothioarsenates by capillary zone electrophoresis and ion chromatography

Schwedt, Georg; Rieckhoff, Melanie

doi:10.1016/0021-9673(95)01319-9

Cited by 63 publications

(55 citation statements)

References 17 publications

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“…A commercially available standard (C 2 H 6 S 3 ; Acros Organics) and fractions of a synthesized dimethylpolysulfane mixture were used for quantification (25). The abiotic monothioarsenate control (Na 3 AsO 3 S·7H 2 O; formula weight, 350) was synthesized as previously described (26,27), and the elemental sulfur (10 g liter Ϫ1 ) and 5 mM arsenite (NaAs III O 2 ; SigmaAldrich Chemical Co.) abiotic control was prepared with base medium and incubated at 75°C for 4 days before derivatization. Samples for arsenic speciation analysis were thawed in an anaerobic hood (95% N 2 , 5% H 2 atmosphere) to minimize oxidation.…”

Section: Methodsmentioning

confidence: 99%

Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park

Jay

Beam

Dohnálková

et al. 2015

Appl Environ Microbiol

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d Thermoproteales (phylum Crenarchaeota) populations are abundant in high-temperature (>70°C) environments of Yellowstone National Park (YNP) and are important in mediating the biogeochemical cycles of sulfur, arsenic, and carbon. The objectives of this study were to determine the specific physiological attributes of the isolate Pyrobaculum yellowstonensis strain WP30, which was obtained from an elemental sulfur sediment (Joseph's Coat Hot Spring [JCHS], 80°C, pH 6.1, 135 M As) and relate this organism to geochemical processes occurring in situ. Strain WP30 is a chemoorganoheterotroph and requires elemental sulfur and/or arsenate as an electron acceptor. Growth in the presence of elemental sulfur and arsenate resulted in the formation of thioarsenates and polysulfides. The complete genome of this organism was sequenced (1.99 Mb, 58% G؉C content), revealing numerous metabolic pathways for the degradation of carbohydrates, amino acids, and lipids. Multiple dimethyl sulfoxide-molybdopterin (DMSO-MPT) oxidoreductase genes, which are implicated in the reduction of sulfur and arsenic, were identified. Pathways for the de novo synthesis of nearly all required cofactors and metabolites were identified. The comparative genomics of P. yellowstonensis and the assembled metagenome sequence from JCHS showed that this organism is highly related (ϳ95% average nucleotide sequence identity) to in situ populations. The physiological attributes and metabolic capabilities of P. yellowstonensis provide an important foundation for developing an understanding of the distribution and function of these populations in YNP.

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Section: Methodsmentioning

confidence: 99%

Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park

Jay

Beam

Dohnálková

et al. 2015

Appl Environ Microbiol

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“…As can be seen from the table, the large majority of studies showed no comparison data. For the few that did, the comparison has been mainly between CZE and IC [3,23,25,41,43,45,63,65,67,70,72,74,76,77,84,86,96,97,104,106,110,111,113,119,124,126,127,131,134,135,140,141,146,148,149,152,158,174,190,194,195,219]. Typical examples include studies by Yang et al [126], who found that results obtained using a high-sensitivity method developed using sample stacking technique together with the use of an internal standard correlated well with a standard IC method, and work by Fung and Lau [149], who examined a CZE method for the analysis of inorganic anions in rainwater and used an IC technique in parallel, reporting that the results for the major anions were within statistical variation.…”

Section: Evaluating Accuracymentioning

confidence: 99%

Quantitative capillary zone electrophoresis of inorganic anions

Paull

King

2003

Electrophoresis

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Despite the availability of commercial capillary electrophoresis systems for over ten years, where quantitative analysis is required, capillary zone electrophoresis (CZE) has often failed to replace ion chromatography as the method of choice for a large number of analytes, not least inorganic anions. To investigate the reasons for this apparent failing, a review is presented of work that has been carried out to-date involving the quantitative application of CZE to the determination of inorganic anions in industrial and environmental samples. This review summarizes work both investigating and improving the quantitative aspects of the CZE of inorganic anions. A complete survey of how CZE has been applied to the determination of inorganic anions in real samples is given, including what, if any, analytical performance parameters were investigated and quoted, and if quality assurance data and validation methods were briefly considered, thoroughly investigated or simply ignored.

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“…Aqueous AsO 4 3À is substitutionally rather inert at ambient temperature, requiring $5 days for 50% exchange of its first-shell O atoms with solvent water (although protonation accelerates this process; Okumura et al, 1995). Judging by their rates of interconversion (Schwedt and Rieckhoff, 1996), As(V) thioanions undergo first-shell ligand exchanges on time scales similar to arsenate in the absence of sulfide/polysulfide. Inertness of As(V) species makes them identifiable by chromatographic methods but impairs their characterization by methods that depend upon attaining rapid equilibrium.…”

Section: Introductionmentioning

confidence: 98%

Thermodynamic model for arsenic speciation in sulfidic waters: A novel use of ab initio computations

Helz

Tossell

2008

Geochimica et Cosmochimica Acta

112

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Separation of thio- and oxothioarsenates by capillary zone electrophoresis and ion chromatography

Cited by 63 publications

References 17 publications

Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park

Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park

Quantitative capillary zone electrophoresis of inorganic anions

Thermodynamic model for arsenic speciation in sulfidic waters: A novel use of ab initio computations

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