Separation of triacylglycerols by supercritical-fluid argentation chromatography

Demirbüker, Mustafa; Blomberg, Lars G.

doi:10.1016/s0021-9673(01)88580-0

Cited by 40 publications

(13 citation statements)

References 13 publications

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“…Columns are prepared from fused silica capillary tubing (Chrompack) of 160 mm [99], 250 mm [98], 290 mm [100], or 330 mm [101] length with 0.25 mm inner diameter, with 0.05 mm silica for the transfer lines. The packed bed is supported by glass fibre filters, and the transfer line to the detector is attached to the column by epoxy glue [99].…”

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

“…The packed column is washed first with 400 lL of a 10% aqueous ammonium nitrate followed by 400 lL of 0.1 M AgNO 3 solution. Washing with ammonium nitrate was rejected in later procedures [100]. Thorough rinsing after the impregnation was of substantial importance for the stability of the stationary phase.…”

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

“…The stationary phase for Ag-SFC is the same as in Ag-HPLC: silica-based cation exchanger Nucleosil TM 4SA [97,98] [100]. Thorough rinsing after the impregnation was of substantial importance for the stability of the stationary phase.…”

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

“…The columns were applied also for separation of TAG according to unsaturation [99 -102]. Regioselective separations have not been reported, but some separation according to chain length has been observed [100].…”

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

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Stationary phases for silver ion chromatography of lipids: Preparation and properties

Momchilova

Nikolova‐Damyanova²

2003

J of Separation Science

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Procedures for the preparation of stationary phases for several silver ion chromatographic techniques have been surveyed. Thin-layer chromatography (TLC), conventional (normal pressure) column chromatography, solid phase extraction (SPE), highperformance liquid chromatography (HPLC), and supercritical fluid chromatography (SFC) in silver ion mode have been considered. The utilization of these stationary phases for the analysis of fatty acids and triacylglycerols has been demonstrated.

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Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

Section: Silver Ion Supercritical Fluid Chromatographymentioning

confidence: 99%

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Stationary phases for silver ion chromatography of lipids: Preparation and properties

Momchilova

Nikolova‐Damyanova²

2003

J of Separation Science

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“…TG separation can also be achieved with supercritical fluid chromatography (SFC) with mobile phases composed of carbon dioxide and a co‐solvent (or modifier). The first reports employed silver‐ion SFC, 14–16 possibly coupled to mass spectrometry 17 . Later, reversed‐phase SFC analysis on C18‐bonded phases was also demonstrated 18–22 .…”

Section: Introductionmentioning

confidence: 99%

Ultra high efficiency/low pressure supercritical fluid chromatography (UHE/LP‐SFC) for triglyceride analysis: Identification, quantification, and classification of vegetable oils

Lesellier

Latos

West

2021

Analytical Science Advances

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Triglycerides of vegetable oils have been extensively studied. Non‐aqueous reversed‐phase liquid chromatography and silver‐ion chromatography are most frequently used to achieve their separation. In previous works, we presented the use of supercritical fluid chromatography with long columns (75 cm) packed with fused‐core particles to provide ultra‐high‐performance separations, with a low‐toxicity fluid (carbon dioxide) compared to the usual liquid‐phase methods. In the present paper, we describe the quantification of triglycerides with supercritical fluid chromatography and evaporative light‐scattering detection. Thanks to the isocratic elution mode, this quantification can be simplified, assuming (a) identical response coefficients for compounds having a close structure, (as only triglycerides are quantified), and (b) constancy of the response coefficient along the analysis (no elution gradient). Therefore, the relative concentrations of triglycerides were easily assessed. Only one calibration curve for one reference compound (in this case triolein) was required. The resulting relative concentrations are in good accordance with the numerous publications available. Relative quantification with UV detection at 210 nm is also proposed, facilitated by the very low UV absorption of carbon dioxide and with a calibration curve taking account of the variation of UV response according to double bond number. Nineteen vegetable oils are compared. The identification of triglycerides was carried out based on previous knowledge of these oils, but also with the help of a Goiffon retention diagram, based on the relationship between the logarithm of retention factor and the total double bond number. Finally, cluster analyses were computed, based on evaporative light‐scattering detection or UV quantification data. They allow a quick comparison of the triglyceride content between the oils, in the goal to exchange one by the other for certain applications, or to compare a new oil to well‐known ones.

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Analysis of triglycerides by capillary electrochromatography

et al. 1997

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Separation of triacylglycerols by supercritical-fluid argentation chromatography

Cited by 40 publications

References 13 publications

Stationary phases for silver ion chromatography of lipids: Preparation and properties

Stationary phases for silver ion chromatography of lipids: Preparation and properties

Ultra high efficiency/low pressure supercritical fluid chromatography (UHE/LP‐SFC) for triglyceride analysis: Identification, quantification, and classification of vegetable oils

Analysis of triglycerides by capillary electrochromatography

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