Separation of Two Cytotoxic Saponins from the Roots of <i>Adenophora triphylla</i> var. <i>japonica</i> by High‐speed Counter‐current Chromatography

Kang, Minseok; Ha, In-Joong; Chun, Jaemoo; Kang, Sam Sik; Kim, Yeong Shik

doi:10.1002/pca.2394

Cited by 20 publications

(15 citation statements)

References 20 publications

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“…The lower phase was then pumped into the head end of the column at a flow rate of 6.0 mL/min while the apparatus was run at a revolution speed of 600 rpm. After the mobile phase front emerged and hydrodynamic equilibrium was established in the column, the sample solution (10 mL) containing 500 mg of crude extract was introduced through the injection valve (Kang, Ha, Chun, Kang, & Kim, 2012). There were multiple attempts using trial and error to adjust a proper concentration of the enriched resveratrol chemotype extracts to observe the particularly different λmax values (Yuk et al, 2013).…”

Section: Hsccc Separationmentioning

confidence: 99%

Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure

Ryu

Song²,

Shin

et al. 2015

Journal of Functional Foods

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Section: Hsccc Separationmentioning

confidence: 99%

Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure

Ryu

Song²,

Shin

et al. 2015

Journal of Functional Foods

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“…1a) was isolated from the root of A. triphylla var. japonica using high-speed counter current chromatography as previously described [17]. Compound 1 was dissolved in DMSO as a stock solution, and the final DMSO concentration was kept below 0.1 % in culture media to ensure that it had no effect on the cells.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…quillaic acid, named compound 1, showed the strongest cytotoxicity in A549, AGS, and HepG2 cancer cells [17]. There is no report concerning the activities and molecular mechanisms employed by compound 1.…”

Section: Introductionmentioning

confidence: 99%

A triterpenoid saponin from Adenophora triphylla var. japonica suppresses the growth of human gastric cancer cells via regulation of apoptosis and autophagy

2014

Self Cite

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In the present study, we investigated the effects of 3-O-β-D-galactopyranosyl-(1 → 2)-[β-D-xylopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl-28-O-[α-L-arabinopyranosyl-(1 → 4)-α-L-arabinopyranosyl-(1 → 3)-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)-β-D-fucopyranosyl] quillaic acid, named compound 1, on the induction of apoptosis and autophagy in human gastric cancer AGS cells. Compound 1, a triterpenoid saponin isolated from the root of Adenophora triphylla var. japonica, effectively inhibited the growth of AGS cells by inducing apoptosis, as well as autophagy. Apoptosis by compound 1 treatment was associated with activation of caspases, release of cytochrome c, and increased ratio of Bax/Bcl-2. Autophagy by compound 1 treatment was indicated by LC3-II protein expression. We also found an increase in phosphorylation of p38 and JNK and a decrease in phosphorylation of ERK and Akt after compound 1 treatment. Furthermore, pretreatment with p38 inhibitor SB202190 completely inhibited compound 1-induced activation of caspases and cleavage of PARP1, whereas pretreatment with SB202190 synergistically increased the protein expression of LC3-II. These results suggest that compound 1 distinctly induces apoptotic and autophagic cell death and the increased autophagy by SB202190 protects compound 1-induced AGS cell death. Our findings provide an important clue for exploring the potential anticancer role of compound 1.

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“…Although the contents slightly fluctuate depending on the growth condition and genotype of individual variety, it has been proved that A. triphylla holds various phytochemicals, such as β-sitosterol, lupenone, daucosterol, triphyllol, and adenophoric acid methyl ester [5][6][7]. Previous research focused on separation, extract, and characterization of those phytochemicals and on the disease-fighting medicinal mechanisms of A. triphylla [3,[8][9][10]. However, there are no studies available reporting on the growth and physiology of plug seedlings of this species.…”

Section: Introductionmentioning

confidence: 99%

Growth and Physiological Responses of Adenophora triphylla (Thunb.) A.DC. Plug Seedlings to Day and Night Temperature Regimes

Ren

Jeong

et al. 2018

Agronomy

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Adenophora triphylla (Thunb.) A.DC., three-leaf lady bell, is an important medicinal plant used against cancers and obesity. It has been well-established that the temperature regime affects plant growth and development in many ways. However, there is no study available correlating the growth of A. triphylla seedlings with different day and night temperature regimes. In order to find an optimal temperature regime, growth and physiology were investigated in A. triphylla plug seedlings grown in environment-controlled chambers at different day and night temperatures: 20/20 °C (day/night) (TA), 25/15 °C (TB), and 20/15 °C (TC). The seedlings in plug trays were grown under a light intensity of 150 μmol·m−2·s−1 PPFD (photosynthetic photon flux density) provided by white LEDs, a 70% relative humidity, and a 16 h (day)/8 h (night) photoperiod for six weeks. The results showed that the stem diameter, number of roots, and biomass were significantly larger for seedlings in TB than those in TA or TC. Moreover, the contents of total flavonoid, total phenol, and soluble sugar in seedlings grown in TB were markedly higher than those in seedlings in the other two treatments. Soluble protein content was the lowest in seedlings in TC, while starch content was the lowest in seedlings grown in TA. Furthermore, seedlings grown in TB showed significantly lower activities of antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase. Native PAGE (polyacrylamide gel electrophoresis) analysis further proved low activities of antioxidant isozymes in TB treatment. Meanwhile, the lowest content of hydrogen peroxide was observed in seedlings grown in TB. In conclusion, the results suggested that the 25/15 °C (day/night) temperature regime is the most suitable for the growth and physiological development of A. triphylla seedlings.

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Separation of Two Cytotoxic Saponins from the Roots of Adenophora triphylla var. japonica by High‐speed Counter‐current Chromatography

Abstract: Two cytotoxic saponins were isolated for the first time from the roots of Adenophora triphylla by HSCCC.

Cited by 20 publications

References 20 publications

Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure

Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure

A triterpenoid saponin from Adenophora triphylla var. japonica suppresses the growth of human gastric cancer cells via regulation of apoptosis and autophagy

Growth and Physiological Responses of Adenophora triphylla (Thunb.) A.DC. Plug Seedlings to Day and Night Temperature Regimes

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