Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins

Li, Hongjuan; Liu, Ying; Huang, Jin; Liu, Sujing; Fang, Shu; Wang, Chunhua; Chen, Xia

doi:10.1016/j.jchromb.2015.10.043

Cited by 11 publications

(6 citation statements)

References 26 publications

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“…Conventional isolation of bioactive compounds is usually performed using high-speed counter-current chromatography and bioassay-guided fractionation 19 . However, these strategies are considered time-consuming, cumbersome, inefficient, and wasteful of organic solvents, while also having low recovery rates and high cost.…”

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confidence: 99%

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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Morus and Broussonetia trees are widely used as food and/or feed. Among 23 phenolics identified from leaves of five Moraceae species using UPLC-QTOF-MS/MS, 15 were screened using DPPH/ABTSguided HPLCs, including seven weak (flavonoids with one hydroxyl on B-ring) and eight strong (four caffeoylquinic acids and four flavonoids, each with a double hydroxyl on B-ring) antioxidants. We then determined the activity and synergistic effects of individual antioxidants and a mixture of the eight strongest antioxidants using DPPH-guided HPLC. Our findings revealed that (1) flavonoid glucuronide may have a more negative effect on antioxidant activity than glucoside, and (2) other compounds in the mixture may exert a negative synergistic effect on antioxidant activity of the four flavonoids with B-ring double hydroxyls but not the four caffeoylquinic acids. In conclusion, the eight phenolics with the strongest antioxidant ability reliably represented the bioactivity of the five extracts examined in this study. Moreover, the Morus alba hybrid had more phenolic biosynthesis machinery than its cross-parent M. alba, whereas the Broussonetia papyrifera hybrid had significantly less phenolic machinery than B. papyrifera. This difference is probably the main reason for livestock preference for the hybrid of B. papyrifera over B. papyrifera in feed.Morus and Broussonetia tree species (family: Moraceae) have high economic value; among other uses, their leaves are widely used as feed to improve meat quality. The genus Broussonetia comprises five species: B. papyrifera, B. kazinoki, B. zeylanica, B. kaempferi, and B. kurzii 1 , some of which are used as folk medicinal plants and/or for making bark paper 2 . The bark, fruits, leaves, and roots of B. papyrifera and B. kazinoki exhibit antioxidant, anti-inflammatory, antinociceptive, and anti-tyrosinase activity 3-6 . In China, B. papyrifera and B. kazinoki leaves, from which several bioactive components such as polyphenols, flavonoids and alkaloids have been isolated 7 , are widely used to feed livestock for meat quality improvement. Methanol extracts from B. papyrifera leaves have greater inhibitory effects on mushroom tyrosinase and 2,2-diphenyl-1-picrylhydrazyl/(DPPH)• scavenging activity 8 , and ethanol extracts from B. papyrifera roots inhibit both acetyl-and butyl-cholinesterases 2 . Papyriflavonol A, isolated from the root bark of B. papyrifera, exhibits strong antimicrobial activity against different pathogenic bacteria and fungi 9 , whereas a 70% ethanol leaf extract of B. kazinoki was reported to dramatically reduce ear and dorsal skin thickness and other clinical symptoms in mice with atopic dermatitis, possibly via the downregulation of immunoglobulin E (IgE) and interleukin-4 (IL-4) plasma levels 5 . Broussonone A extracted from B. kanzinoki stem bark displays noncompetitive inhibitory activity against pancreatic lipase 10 . However, data on the antioxidant activity and bioactive composition of B. kanzinoki remains limited.The genus Morus comprises 10-16 species 11 , of whic...

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confidence: 99%

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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“…[1], with the effects of activating blood flow and removing blood stasis [2][3][4][5]. Hawthorn leaf extract is a liquid obtained by extracting hawthorn leaf powder with 50% ethanol and elution with D101 macroporous resin or extracted by ultrasonic extraction after adding 60% methanol [6][7][8]. It has been reported that hawthorn leaf extract can reduce blood plasma viscosity, promote hemoglobin degradation, and increase the number of platelets to possess therapeutic effects on the cardiovascular systems [9].…”

Section: Introductionmentioning

confidence: 99%

“…Vitexin-4"-O-glucoside (VOG), Vitexin-2"-Orhamnoside (VOR), vitexin (VIT), and hyperoside (HP), as the main components in hawthorn leaf extract, are important active substances that exert their medicinal effects. It is reported that both VOG and VOR can significantly improve the toxicity and apoptosis of mice, with a positive effect on avoiding H 2 O 2 -mediated oxidative stress damage at the same time [10]; VOR exerted potent antithrombotic activity in vitro [6]; It is well known that VIT has a wide range of pharmacological effects, including anti-inflammatory, antioxidant, anti-hyperalgesic and neuroprotective effects, and even anti-diabetics, anti-Alzheimer's disease, and anti-cancer effects [11,12], moreover, both in vitro and in vivo studies suggested that VIT is a chemopreventive compound with activity against various cancers through proapoptotic processes and/or autophagy [13]; HP can inhibit high glucose-induced vascular inflammation [3], protect acetaminophen-induced liver injury [14], and inhibit the proliferation of pancreatic cancer and lung cancer cells [15,16]. Currently, the main research methods of flavonoids in hawthorn leaves include high-performance liquid chromatography (HPLC) [17,18], HPLC-mass spectrometry (HPLC-MS) [19], ultra-high performance liquid chromatography-tandem MS (UHPLC-MS/MS) [20], etc.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of four flavonoids from hawthorn leaves in plasma by ultra‐high performance liquid chromatography‐tandem mass spectrometry and study on its pharmacokinetics

Xu,

Zhou,

et al. 2024

Separation Science Plus

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In this study, a rapid and sensitive ultra‐high performance liquid chromatography‐tandem mass spectrometry method was established for the simultaneous determination of four Vitexin‐4“‐O‐glucoside (VOG), Vitexin‐2”‐O‐rhamnoside (VOR), vitexin (VIT), and hyperoside (HP) in plasma, which are representative flavonoids constituents of hawthorn leaves. The four components were separated by CORTECS UPLC C18 column (100 × 3.0 mm, 1.6 µm), using acetonitrile‐water containing 0.02% formic acid and 2 mmol/L ammonium formate as the mobile phase, the gradient elution flow rate was 0.30 mL/min. The analyte was quantified using an electrospray ionization triple quadrupole mass spectrometer. The analytes and baicalin internal standard were performed at m/z 593.2/413.2 for VOG, m/z 577.2/413.1 for VOR, m/z 431.1/310.9 for VIT, m/z 463.2/300 for HP and m/z 445.1/269 for internal standard. Among the four flavonoids, the correlation coefficient of the standard curve of each component is between 0.9939 and 0.9987. After oral administration of hawthorn leaf extract, VOG and VOR showed similar pharmacokinetic characteristics; VIT was eliminated in the body more slowly than the other three flavonoids and distributed more in the tissues; HP can be quickly absorbed in the blood. The developed analytical method has high sensitivity, precision, and accuracy with no interference from plasma components and has been successfully used in pharmacokinetic studies.

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“…A variety of hawthorn leaf preparations, such as Yixintong and Shanmei capsule, is clinically used to treat cardiovascular diseases such as hyperlipidemia, coronary heart disease, angina pectoris, and arrhythmia [ 14 , 15 ]. At present, a great many types of chemical constituents have been extracted from hawthorn leaves, including flavonoids, flavane and its polymers, pentacyclic triterpenes, monoterpenes, sesquiterpenes, lignans, organic acids, volatile oil, and so on [ 10 , 16 , 17 ], among which flavonoids are considered being the main active ingredients of hawthorn leaves and important ingredients for the herb to exert its drug activity [ 18 , 19 ]. Our previous study [ 20 ] found that hawthorn leaf flavonoids (HLF) can reduce the levels of blood lipid and improve the liver function in hyperlipidemic mice.…”

Section: Introductionmentioning

confidence: 99%

The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats

Weng

Cui

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. The purpose of this study was to explore the potential mechanisms of the lipid-regulating effects and the effect on modulating the gut microbiota of hawthorn leaf flavonoids (HLF) in the high-fat diet-induced hyperlipidemic rats. Methods. The hypolipidemic effect of HLF was investigated in the high-fat diet-induced hyperlipidemic rats. The action targets of HLF in the treatment of hyperlipidemia were predicted by network pharmacology and KEGG enrichment bubble diagram, which were verified by the test of western blotting. Meanwhile, we used 16S rRNA sequencing to evaluate the effects of HLF on the microbes. Results. The results of animal experiments showed that HLF could reduce the body weight and regulate the levels of serum lipid in high-fat diet (HFD) rats. Meanwhile, for the related targets of cholesterol metabolism, HLF could significantly upregulate the expression of LDLR, NR1H3, and ABCG5/ABCG8; reduce the expression of PCSK9; and increase the level of CYP7A1 in the intestinal tissue, whereas cholesterol biosynthetic protein expressions including HMGCR and SCAP were lowered by HLF. In addition, HLF increased the activities of plasma SOD, CAT, and GSH-Px and decreased the levels of Casp 1, NLRP3, IL-1β, IL-18, and TNF-α, improving the degree of hepatocyte steatosis and inflammatory infiltration of rats. Notably, HLF significantly regulated the relative abundance of major bacteria such as g_Lactobacillus, g_Anaerostipes, g_[Eubacterium]_hallii_group, g_Fusicatenibacter, g_Akkermansia, and g_Collinsella. Synchronously, we found that HLF could regulate the disorder of plasma HEPC and TFR levels caused by HFD. Conclusion. This study demonstrates that HLF can regulate metabolic hyperlipidemia syndromes and modulate the relative abundance of major bacteria, which illustrated that it might be associated with the modulation of gut microbiota composition and metabolites.

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Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins

Cited by 11 publications

References 26 publications

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Rapid determination of four flavonoids from hawthorn leaves in plasma by ultra‐high performance liquid chromatography‐tandem mass spectrometry and study on its pharmacokinetics

The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats

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