Separation of yeast cells from aqueous solutions using magnetically stabilized fluidized beds

Abstract

Aims: To separate Saccharomyces cerevisiae cells from aqueous solutions using magnetically stabilized fluidized beds (MSFB) that utilize a horizontal magnetic field, and to study the effect of some parameters, such as bed porosity and height, liquid flow rate and inlet concentration on cell removal efficiency and breakthrough curves. Methods and Results: The separation process was conducted in an MSFB under the effect of horizontal magnetic field. The magnetic particles used consist of a ferromagnetic core of magnetite (Fe3O4) covered by a stable layer of activated carbon to adsorb the yeast cells from the suspension. The yeast cell concentration in the effluent was determined periodically by measuring the absorbance at 610 nm. The effect of the magnetic field intensity on the bed porosity and consequently the exit‐normalized cell concentration from the bed was studied. It was found that bed porosity increased by 75%, and the normalized cell concentration in the bed effluent decreased by 30%, when the magnetic field intensity was increased from 0 to 110 mT. In addition, increasing the magnetic field intensity and bed height delayed the breakthrough point, and allowed efficient cell removal. These results demonstrate an improved method to separate cells of low concentration from cell suspension. Conclusions: This study allows the continuous separation of yeast cells from aqueous solutions in an MSFB. The removal efficiency is affected by different parameters including the bed height, flow rate and initial concentration. The removal efficiency reaches 82%, and could be improved by varying the operational parameters. Significance and Impact of the Study: The results obtained in this investigation show that the MSFB using horizontal fields represents a potential tool for the continuous separation of cell suspension from aqueous solution. This study will contribute to a better understanding of the hydrodynamic parameters on the separation efficiencies of the cell.

Magnetically responsive yeast cells: Methods of preparation and applications

“…The removal efficiency was affected by different parameters, including the bed height, flow rate and initial concentration of cells. The removal efficiency reached 82%, and could be improved by varying the operational parameters (Al‐Qodah and Al‐Shannag, ).…”

An Extensive Analysis of Combined Processes for Landfill Leachate Treatment

“…• The utilization of kinetic and statistical models to comprehensively describe the various combined processes is currently constrained. However, if these models accurately depict the experimental outcomes, they hold the potential for application in the upscaling of such systems [29,[175][176][177][178].…”

Mass transfer intensification between fluidizing gas and Geldart‐B nonmagnetizable particles in magnetized fluidized bed

“…Moreover, the contact between the fluidizing liquid and magnetizable particles could also be strengthened by the magnetic field in the magnetic stabilization flow regime as compared with that in conventional liquid–solid fluidized beds. The liquid–solid MSB has been extensively investigated in the area of biotechnology, such as the fermentation broth processing 27,28 and subsequent separation via affinity chromatography 29,30 or adsorption 31,32 . Additionally, more and more researchers focused on the enzyme/cell immobilization on the magnetizable particles 33–38 to take the advantages of the MSB.…”