Separation, purification of anthocyanin and vitis linn polysaccharide from grape juice by the two-step extraction and dialysis

Abstract

Liquid liquid extraction, as a clean and green process, has a great potential in technology and innovation, which has been widely used in industrial production. In this paper, a liquid-liquid extraction process based on L44-NaH 2 PO 4 ATPS was studied for separation and purification of anthocyanin and vitis linn polysaccharide from the grape juice. The concentrations of L44 and NaH 2 PO 4 , temperature and pH as factors of effect on the extraction rates were discussed. Under the optimal conditions, the extraction rates of anthocyanin and vitis linn polysaccharides were 99.8% and 90.3%, respectively. The work subject is extraction of two compounds from grape juice, which can be very important for food, pharmaceutical and cosmetic industries. The vitis linn polysaccharides were further purified by dialysis and the impurity protein was successfully removed based on organic solvent precipitation method. Besides, the recycle of L44 was also achieved through secondary extraction. Practical applicationsA liquid-liquid extraction process based on L44-NaH 2 PO 4 ATPS was studied for separation and purification of anthocyanin and vitis linn polysaccharide from the grape juice. The efficient separation of two kinds of water-soluble components is of great significance in industrial production. It is very interesting for scientific communities and shows a great biotechnological potential of the ATPS purification process. The work subject is extraction of two compounds from grape juice, which can be very important for food, pharmaceutical and cosmetic industries.

Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction

“…This result suggests that anthocyanins migrated preferentially to the alcohol-rich phase when salt and alcohol concentrations in the system were increased. Higher affinity for the top phase has been observed for the extraction and purification of Dacrydes peel anthocyanins in ATPS composed of ethanol and ammonium sulfate/dipotassium phosphate/potassium dihydrogen phosphate (Al-Alawi et al, 2018) as well as for the extraction and pre-purification of grape juice anthocyanins in ethanol/sodium phosphate ATPS (Tang et al, 2018). Liu et al (2013) also observed an increase in the partition coefficient of purple sweet potato anthocyanins in ethanol/ammonium sulfate ATPS when salt concentration was increased.…”

Flash Extraction, Characterization, and Immunoenhancement Activity of Polysaccharide fromHippophae rhamnoidesLinn

“…The dissolved CHRP (10 mg/mL) solution was slowly and uniformly dropped into the cotton DEAE chromatography column, [45] then eluted with 0.6 mol/L NaCl solution at a flow rate of 1 mL/min. The eluent solution was placed in distilled water in a treated dialysis bag for 48 h [46] . The distilled water was changed every 3–4 h. The polysaccharide concentrate in the dialysis bag was then freeze‐dried to obtain the preliminary purified Hippophae rhamnoides polysaccharide, named PHRP.…”