Separation, purification, structural analysis and immune-enhancing activity of sulfated polysaccharide isolated from sea cucumber viscera

Purification and antioxidant and anti‐Inflammatory activity of extracellular polysaccharopeptide from sanghuang mushroom, Sanghuangporus lonicericola

“…First, 150 ml of the fermented PDB broth was concentrated at 60 °C in a rotary evaporator to 25 mL. To precipitate SePSP, 24 absolute ethanol was then added to the concentrated broth at a ratio of 4:1 (v/v), followed by thorough mixing. After allowing the mixture to stand for 24 h, crude SePSP was collected by centrifugation at 6000× g for 5 min and removal of the supernatant.…”

Physicochemical and biological activities of polysaccharides from the waste residue of sea cucumber peptide production

“…Nevertheless, the remaining residues containing polysaccharides and saponins became waste after extraction, enzymatic hydrolysis, and filtration of proteins and peptides. Sea cucumber polysaccharides are essential constituents of the sea cucumber wall, and have abundant biological activities including antioxidant, anti-tumour, anti-coagulant, anti-inflammatory, antithrombotic and anti-hyperlipidemic activities (Liu et al, 2012;Yang et al, 2019). Two novel sulphated polysaccharides have been isolated from sea cucumber body: fucosylated chondroitin sulphate and sulphated fucan (Albano and Mourão, 1986).…”

Isolation, Purification and Structural Characteristics of Glycosaminoglycans From Sea Cucumber Stichopus Horrens

“…The band at 2927.69 and 2937.36 cm-1 reflect the stretching vibration of C-H representing methyl group of fucose, that suggesting the presence of fucose in both fraction F1 and F2 [19]. Signals at 1643 cm -1 and 1033 cm -1 (Figure 3A) were assigned to the asymmetric stretching vibration of C=O (carboxyl) and C-O-C, respectively, that belong to uronic acid group [18]. The signal at 1641 cm -1 (Fig.…”

“…Previous studies reported that sea cucumber glycosaminoglycans usually divided into two types (Fucosylated Chondroitin Sulfate-FCS and Fucan Sulfate-FS) such as glycosaminoglycans (polysaccharides) from sea cucumbers H. nobilis, A. japonica [16]; H. Polii [17]; A. molpadioidea, H. Nobilis [2]; P. graeffei, H. vagabunda, S. tremulus, I. badionotus [4], and H. atra, H. arenicola, S. horrens [14], except polysaccharides from H. edulis obtained three fractions including two FCS fractions and one FS fraction [14]. * IR spectroscopy: The IR spectrum of F1 and F2 fraction were shown in Figure 3, the signals at 3448.95 and 3446.46 cm -1 with a broad intense peak that attributed to the stretching of O-H [18]. The band at 2927.69 and 2937.36 cm-1 reflect the stretching vibration of C-H representing methyl group of fucose, that suggesting the presence of fucose in both fraction F1 and F2 [19].…”

“…Three signal groups in IR spectrum of F1 (Figure 3A) were assigned to the sulfate groups, particularly, those appeared at 1250.32 cm -1 correspond to S=O asymmetric stretching vibration, those at 825.37, 848.38 cm -1 were assigned to the symmetric C-O-S stretching vibration [18], which was the C4 axial position of the fucopyranose ring (4-O-sulfated-Fuc or 4-O-sulfatedgalnac), and those at 586.03 cm -1 was caused by S-O stretching vibration, which showed that the sulfate group also had a small amount at the C2 and/or C3 positions [18]. IR spectrum of F2 (Figure 3B) showed strong absorptions at 1233 cm -1 (S=O) and 842 cm -1 to indicate the presence of 4-O-sulfated-Fuc, as according to previous reports of fucan sulfate [2,18]. * NMR spectroscopy: Similarly, the NMR spectrum of fucosylated chondroitin sulfate (FCS) of other sea cucumber species in the world has been published [1,14,15,17].…”