Separation selectivity of some phenolic acids in RP HPLC systems with binary mobile phase comprised various modifiers

Klimek-Turek, Anna; Dzido, Tadeusz H.

doi:10.1007/s10450-010-9241-2

Cited by 11 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For sufficient retention of acids on reversed phase their protonated state is a prerequisite [21], while basic (i.e. deprotonating) additives enhance ionization in an ESI source operating in negative ion mode [22].…”

Section: Resultsmentioning

confidence: 99%

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

et al. 2012

View full text Add to dashboard Cite

BackgroundPhytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resistance to abiotic stress (drought, UV-irradiation and mechanical wounding). These factors induce dramatic changes in phytohormone biosynthesis and transport leading to rapid local and systemic stress responses. Understanding of underlying mechanisms is of principle interest for scientists working in various areas of plant biology. However, highly sensitive, precise and high-throughput methods for quantification of these phytohormones in small samples of plant tissues are still missing.ResultsHere we present an LC-MS/MS method for fast and highly sensitive determination of jasmonates, abscisic and salicylic acids. A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. This strategy resulted in dramatic increase in overall sensitivity, allowing successful determination of phytohormones in small (less than 50 mg of fresh weight) tissue samples. The method was completely validated in terms of analyte recovery, sensitivity, linearity and precision. Additionally, it was cross-validated with a well-established GC-MS-based procedure and its applicability to a variety of plant species and organs was verified.ConclusionThe method can be applied for the analyses of target phytohormones in small tissue samples obtained from any plant species and/or plant part relying on any commercially available (even less sensitive) tandem mass spectrometry instrumentation.

show abstract

Section: Resultsmentioning

confidence: 99%

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Therefore, a simple approach that enables interpretation of retention changes and putting forward clear conclusions is much desired. Such an approach was proposed earlier to explain the changes in separation selectivity of aromatic hydrocarbons with polar groups [6] and phenolic acids [7, 8]. It assumes that selectivity changes generated by the change of the modifier in the eluent can be explained taking into consideration only molecular interactions between the solute and modifier in the stationary phase (interactions in the mobile phase are neglected) and also taking into account ordering of the stationary phase by the modifier [6, 9].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Retention of Aliphatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Modifiers of the Binary Eluent

2013

Self Cite

View full text Add to dashboard Cite

The retention of aliphatic hydrocarbons with polar groups has been compared in respect to the separation selectivity changes in reversed-phase high-performance liquid chromatography with C18 stationary phase type and binary water eluent composed of methanol, acetonitrile, or tetrahydrofuran as modifiers. The changes in separation selectivity when one modifier is replaced by another in the eluent is explained, taking into consideration molecular interactions of the solutes with components of the stationary phase region, i.e., extracted modifier, and ordering of the stationary phase by the modifier.

show abstract

“…Phenolic compounds are a component of lignins and they can be produced as a result microbiological biosynthesis from aliphatic substrates 5,9,[36][37][38] . Changes in the content of lignins and the level of their transformation in decomposing material can be evaluated with the oxidation of plant material and soil samples by using CuO, or applying the method of acid or/and alkaline hydrolysis 36,[39][40][41][42][43][44] . With those methods it is possible to release aldehydes and phenolic acids from lignins.…”

mentioning

confidence: 99%

Transformations of phenolic compounds in meadow soils

Ziolkowska

Dębska

Banach-Szott

2020

Sci Rep

View full text Add to dashboard Cite

The aim of the research has been to determine the role of phenolic compounds in the processes of transformations of organic matter in meadow soils, leading to the formation of humic substances. The research has been performed based on the plant material and soil sampled from Europe’s unique complex of permanent grasslands irrigated continuously for 150 years applying the slope-and-flooding system, the Czerskie Meadows. Phenolic compounds were isolated from the plant material samples (hay, sward and roots) and soils (horizon A, AE and Bsv) and from the fraction of humic and fulvic acids. It was found that the contents of phenolic compounds decrease in the following order: hay > sward > roots > A horizon soil > AE horizon soil > Bsv horizon soil > A horizon fulvic acids > AE horizon fulvic acids > Bsv horizon fulvic acids > A horizon fulvic acids > AE horizon fulvic acids > Bsv horizon fulvic acids. A significantly higher share of cinnamyl than vanillyl and syringyl compounds in the extracts of fulvic acids and slightly higher in the hydrolysates of humic acids confirms the effect of the chemical composition of the plant material undergoing decomposition on the properties of the emerging humic substances.

show abstract

Separation selectivity of some phenolic acids in RP HPLC systems with binary mobile phase comprised various modifiers

Cited by 11 publications

References 18 publications

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

Comparison of the Retention of Aliphatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Modifiers of the Binary Eluent

Transformations of phenolic compounds in meadow soils

Contact Info

Product

Resources

About