Sepsis Induced by Cecal Ligation and Puncture

Wen, Haitao

doi:10.1007/978-1-62703-481-4_15

Cited by 37 publications

(31 citation statements)

References 41 publications

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“…The animal model of CLP developed hypotension and tachycardia as typically seen in human septic shock [ 27 , 28 ]. In the present study, the infusion of LS increased pulse pressure in sham- and CLP-treated rats, suggesting this is due to its positive inotropic effects.…”

Section: Discussionmentioning

confidence: 99%

Levosimendan attenuates multiple organ injury and improves survival in peritonitis-induced septic shock: studies in a rat model

Tsao

Chen

et al. 2014

Crit Care

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Introduction: The aim of this study was to investigate the effects of levosimendan on rodent septic shock induced by cecal ligation and puncture (CLP). Methods: Three hours after peritonitis-induced sepsis, male Wistar rats were randomly assigned to receive an intravenous infusion of levosimendan (1.2 μg/kg/min for 10 min and then 0.3 μg/kg/min for 6 h) or an equivalent volume of saline and vehicle (5% dextrose) solution. Results: The levosimendan-treated CLP animals had significantly higher arterial pressure and lower biochemical indices of liver and kidney dysfunction compared to the CLP animals (P < 0.05). Plasma interleukin-1β, nitric oxide and organ superoxide levels in the levosimendan-treated CLP group were less than those in CLP rats treated with vehicle (P < 0.05). In addition, the inducible nitric oxide synthase (iNOS) in lung and caspase-3 expressions in spleen were significantly lower in the levosimendan-treated CLP group (P < 0.05). The administration of CLP rats with levosimendan was associated with significantly higher survival (61.9% vs. 40% at 18 h after CLP, P < 0.05). At postmortem examination, the histological changes and neutrophil filtration index in liver and lung were significantly attenuated in the levosimendan-treated CLP group (vs. CLP group, P < 0.05).

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Section: Discussionmentioning

confidence: 99%

Levosimendan attenuates multiple organ injury and improves survival in peritonitis-induced septic shock: studies in a rat model

Tsao

Chen

et al. 2014

Crit Care

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“…CLP surgerywas performed as descried previously( 27 ). Briefly, prior to CLP surgery, autoclaved the scissors and forceps, and anesthetized the mice with pentobarbital sodium (45 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

G Protein-Coupled Receptor 109A and Host Microbiota Modulate Intestinal Epithelial Integrity During Sepsis

Chen

Huang

et al. 2018

Front. Immunol.

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The intestinal epithelial barrier is important to mucosal immunity, although how it is maintained after damage is unclear. Here, we show that G protein-coupled receptor 109A (GPR109A) supports barrier integrity and decreases mortality in a mouse cecum ligation and puncture (CLP) sepsis model. Data from 16S RNA sequencing showed that the intestinal microbiota of WT and Gpr109a−/− mice clustered differently and their compositions were disrupted after CLP surgery. GPR109A-deficient mice showed increased mortality, intestinal permeability, altered inflammation, and lower tight junction gene expression. After eliminating the intestinal flora with antibiotics, all experimental mice died within 48 h of CLP surgery. This demonstrates the critical role of the gut microbiota in CLP-induced sepsis. Importantly, mortality and other pathologies in the model were decreased after Gpr109a−/− mice received WT gut microbiota. These findings indicate that GPR109A regulates the gut microbiota, contributing to intestinal epithelial barrier integrity and decreased mortality in CLP-induced sepsis.

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“…To induce polymicrobial sepsis, we used an established murine model of CLP as previously described. [12][13][14] The mice were an aesthetised with sodium pentobarbital (100 mg kg À1 , intraperitoneal). The peritoneum was opened, and the bowel was exposed.…”

Section: Murine Model Of Sepsismentioning

confidence: 99%

Protective role of fenofibrate in sepsis-induced acute kidney injury in BALB/c mice

et al. 2018

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Sepsis Induced by Cecal Ligation and Puncture

Cited by 37 publications

References 41 publications

Levosimendan attenuates multiple organ injury and improves survival in peritonitis-induced septic shock: studies in a rat model

Levosimendan attenuates multiple organ injury and improves survival in peritonitis-induced septic shock: studies in a rat model

G Protein-Coupled Receptor 109A and Host Microbiota Modulate Intestinal Epithelial Integrity During Sepsis

Protective role of fenofibrate in sepsis-induced acute kidney injury in BALB/c mice

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