Septal pore apparatus and nuclear division of Auriscalpium vulgare

Abstract

Ultrastructure of the septal pore apparatus and nuclear division of Auriscalpium vulgare (Russulales) was examined with freeze substitution and is presented for inclusion in the AFTOL Structural and Biochemical Database (http://aftol.umn.edu). Previously unreported septal characters for the Russulales (Agaricomycotina) were observed: Septa of the hymenophore had bell-shaped perforated septal pore caps that may extend along the septum and a zone of organelle exclusion surrounded the septal pore apparatus. Metaphase I of meiosis and metaphase of mitosis were similar. Globular spindle pole bodies with electron-opaque inclusions were set within polar fenestrae of the nuclear envelope. The nuclear envelope was mostly intact with occasional gaps. Fragments of endoplasmic reticulum were present near the spindle pole bodies but did not form a polar cap. Structural characters may distinguish one or more clades of the Agaricomycotina and provide additional signal in phylogenetic analyses.

Evolution of zygomycetous spindle pole bodies: Evidence from Coemansia reversa mitosis

“…Samples were slowly infiltrated for embedding following the infiltration steps for chemical fixation. Embedding, polymerization, cell selection, sectioning, poststaining, and imaging were as in Celio et al (2007) except that polymerization was carried out at 70°C for 14 h, and uranyl acetate formulation was 2% w/v in 50% ethanol.…”