Septicemia Caused by <i>Herbaspirillum huttiense</i> Secondary to Pneumonia

Liu, Changseung; Kwon, Mi; Kim, Myung-Sook; Byun, Jung Hyun; Yong, Dongeun; Lee, Kyungwon

doi:10.3343/alm.2019.39.3.340

Cited by 17 publications

(14 citation statements)

References 8 publications

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“…Abreu-Di Berardino et al described Herbaspirillum huttiense pneumonia in a patient with essential thrombocytosis [9]. Liu et al reported Herbaspirillum huttiense bacteremia in an elderly patient with no obvious immune suppression, who later went on to develop a pneumonia, where despite adequate treatment, microbiological eradication was not easily achieved, and septicemia lasted for several days along with sputum culture positivity for Herbaspirillum huttiense 2 months later [10]. Chemaly were Herbaspirillum seropedicae, and 2 were Herbaspirillum putei, and the remaining eighteen isolates could not be speciated [4].…”

Section: Discussionmentioning

confidence: 99%

“…More recently, some Herbaspirillium species have transitioned from environment (water, contaminated soil, plant internal tissues, and root nodules) to human hosts, mostly as opportunistic (pathogenic) bacteria. Cases of human colonization and infection have mostly been noted in cystic fibrosis and immunocompromised cancer patients [3][4][5][6][7][8] and also in some patients without an apparent immunosuppressed state [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Herbaspirillum Infection in Humans: A Case Report and Review of Literature

Dhital

Paudel

Bohra

et al. 2020

Case Reports in Infectious Diseases

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Introduction. Herbaspirillum seropedicae are Gram-negative oxidase-positive nonfermenting rods of Betaproteobacteria class, commonly found in rhizosphere. More recently, some Herbaspirillium species have transitioned from environment to human hosts, mostly as opportunistic (pathogenic) bacteria. We present a 58-year-old female with non-small-cell lung cancer (NSCLC) who presented with pneumonia and was found to have Herbaspirillum seropedicae bacteremia. Case History. A 58-year-old woman with NSCLC on Pralsetinib presented with fevers and rigors for 2 days. Coarse breath sounds were auscultated on the right upper lung field. Labs revealed leukopenia and mild neutropenia. CT chest revealed right upper lobe pneumonia. She was admitted for sepsis secondary to pneumonia and placed on broad spectrum antibiotics with intravenous piperacillin-tazobactam and vancomycin. The patient continued to have fever 2 days after admission (max: 102.8°F). Preliminary blood cultures grew Gram-negative rods. The patient continued to have temperature spikes on the 3rd day of antibiotics (Tmax 101.5°F). Blood cultures revealed oxidase-positive nonfermenting rods. The patient’s antibiotic was changed to IV meropenem on the 4th day of hospitalization. Ultimately, on the seventh day of hospitalization, the blood culture was confirmed from outside lab as Herbaspirillum seropedicae. The patient started feeling better and defervesced after about 24 hours. Discussion. More recently, Herbaspirillum spp. have been recovered from humans. Our patient had Herbaspirillum bacteremia, and reported regularly cleaning her pond and weeding her garden with possible exposure to this environmental proteobacterium. Herbaspirillum may be more prevalent than earlier thought owing to misidentification. With the institution of appropriate antimicrobial therapy, the outcomes seem mostly favorable.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Herbaspirillum Infection in Humans: A Case Report and Review of Literature

Dhital

Paudel

Bohra

et al. 2020

Case Reports in Infectious Diseases

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“…These isolates originated in Germany, Sweden, and the United States, the oldest isolate being identified in 1968 [ 2 ]. Recently, isolates of the genus Herbaspirillum have been found more frequently in humans, especially in samples from immunocompromised patients with cancer, sepsis, cystic fibrosis, and pneumonia not related to cystic fibrosis [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. The first clinical isolate of H. frisingense to undergo genomic sequencing was strain VT-16–41, which was isolated from a patient with a urinary tract infection [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs

et al. 2021

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The genus Herbaspirillum is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the Herbaspirillum genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria to switch environments and become able to colonize human hosts is essential for monitoring emerging pathogens and predicting outbreaks. In this work, we describe the sequencing, assembly, and annotation of the genome of H. frisingense AU14559 isolated from the sputum of patients with cystic fibrosis, and its comparison with the genomes of the uropathogenic strain VT-16–41 and the environmental strains GSF30, BH-1, IAC152, and SG826. The genes responsible for biological nitrogen fixation were absent from all strains except for GSF30. On the other hand, genes encoding virulence and host interaction factors were mostly shared with environmental strains. We also identified a large set of intrinsic antibiotic resistance genes that were shared across all strains. Unlike other strains, in addition to unique genomic islands, AU14559 has a mutation that renders the biosynthesis of rhamnose and its incorporation into the exopolysaccharide unfeasible. These data suggest that H. frisingense has characteristics that provide it with the metabolic diversity needed to infect and colonize human hosts.

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“…More recently, some Herbaspirillium species have transitioned from the environment to human hosts, mostly as opportunistic bacteria [13][14][15]. Cases of colonization and infection in humans have been noted in cystic fibrosis, immunocompromised cancer patients and cases of pneumonia as well as in some patients without an apparent immunosuppressed state [13][14][15][16][17][18][19][20][21][22]. To better understand how the Herbaspirillum genus are migrating from the environment to human hosts, the genome of Herbaspirillum seropedicae strains isolated from cystic fibrosis patients and from the environment have been sequenced and compared [21].…”

Section: Introductionmentioning

confidence: 99%

Environmental and clinical isolates of Herbaspirillum induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells

Olszewski

Galvão

LiPuma

et al. 2021

Journal of Medical Microbiology

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Introduction. In recent years, the Herbaspirillum genus has emerged as a pathogen in healthcare-related infections and has became stablished as an opportunistic pathogen. Hypothesis/Gap Statement. Little is known about the pathogenesis induced by Herbaspirillum genus. Aim. To evaluate the cytotoxic effects of genus Herbaspirillum , its ability to adhere to lung human cells and the ability of environmental and clinical strains of Herbaspirillum to induce pneumonia in mice. Methodology. Environmental and clinical isolates of Herbaspirillum were examined for their cytotoxic effects on the Calu-3 cell lineage. Cytotoxic activity of secretome was tested using MTT/neutral red assays and cell morphology analysis. Herbaspirillum adhesion on Calu-3 cells was assessed using bright-field microscopy and cell-associated bacteria were counted. A mouse model of acute lung infection was done using a clinical and an environmental strain. Adult male mice were used, and the pneumonia was inducted by intra-tracheal inoculation of 108 or 109 bacteria. Mice weight variations were evaluated at the end of the experiment. Bronchoalveolar lavage was collected and evaluated for total and differential cytology. A histological examination of lungs was performed giving a histological score. Results. The secretomes of all the strains induced morphological alterations in cells, but only H. seropedicae SmR1 were cytotoxic in MTT and neutral red assays. Clinical strains of H. frisingense AU14459 and H. hutttiense subsp. huttiense AU11883 exhibited low adherence to lung cells, while SmR1 was non-adhesive. Following intratracheal inoculation, mice treated with 109 c.f.u. of the SmR1 and AU11883 strains lost 18 and 6% of their weight over 7 days, respectively, and presented moderate clinical signs. Infected mice showed inflammatory cell infiltration in the perivascular and peribroncheal/peribronchiolar spaces. Bronchoalveolar fluid of mice inoculated with SmR1 109 c.f.u. presented an increase in total leucocyte cells and in neutrophils population. Conclusion. These in vivo and in vitro results provide insights into how some Herbaspirillum strains cause infection in humans, providing a basis for the characterization of pathogenesis studies on this emerging infectious agent.

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Septicemia Caused by Herbaspirillum huttiense Secondary to Pneumonia

Cited by 17 publications

References 8 publications

Herbaspirillum Infection in Humans: A Case Report and Review of Literature

Herbaspirillum Infection in Humans: A Case Report and Review of Literature

High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs

Environmental and clinical isolates of Herbaspirillum induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells

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