Septin Expression in Proliferative Retinal Membranes

Xin, Xiaorong; Pache, Mona; Zieger, Barbara; Bartsch, Ingrid; Prünte, Christian; Meyer, Peter

doi:10.1369/jhc.7a7188.2007

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…Furthermore, in contrast to sept3 and sept5b , no sept5a expression is detectable in the retina at 4 dpf. The observed expression of sept5b in the zebrafish retina is in accordance with immunohistochemistry studies of SEPT5 in human eye tissues (Pache et al, 2005 ; Xin et al, 2007 ), which suggests an important conserved role of Sept5 in retinal development (Xin et al, 2007 ). The sept5b expression differs from the expression of sept3 in the retina.…”

Section: Discussionsupporting

confidence: 82%

Expression of sept3, sept5a and sept5b in the Developing and Adult Nervous System of the Zebrafish (Danio rerio)

2017

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Septins are a highly conserved family of small GTPases that form cytoskeletal filaments. Their cellular functions, especially in the nervous system, still remain largely enigmatic, but there are accumulating lines of evidence that septins play important roles in neuronal physiology and pathology. In order to further dissect septin function in the nervous system a detailed temporal resolved analysis in the genetically well tractable model vertebrate zebrafish (Danio rerio) is crucially necessary. To close this knowledge gap we here provide a reference dataset describing the expression of selected septins (sept3, sept5a and sept5b) in the zebrafish central nervous system. Strikingly, proliferation zones are devoid of expression of all three septins investigated, suggesting that they have a role in post-mitotic neural cells. Our finding that three septins are mainly expressed in non-proliferative regions was further confirmed by double-stainings with a proliferative marker. Our RNA in situ hybridization (ISH) study, detecting sept3, sept5a and sept5b mRNAs, shows that all three septins are expressed in largely overlapping regions of the developing brain. However, the expression of sept5a is much more confined compared to sept3 and sept5b. In contrast, the expression of all the three analyzed septins is largely similar in the adult brain.

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Section: Discussionsupporting

confidence: 82%

Expression of sept3, sept5a and sept5b in the Developing and Adult Nervous System of the Zebrafish (Danio rerio)

2017

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“…Studies of mammalian septins have been primarily focused on their association with cancer and neurological diseases. However, many other interesting functions are coming to light such as the role of certain septins in bacterial infection (44)(45)(46), angiogenesis (72), spermatogenesis (40)(41)(42)(43)101), retinal degeneration (102,103), platelet release reaction (32)(33)(34)(35)104), and beyond. Still, questions related to their normal function in cells and to their implication in human diseases are still puzzling.…”

Section: Resultsmentioning

confidence: 99%

Conquering the complex world of human septins: implications for health and disease

2010

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Septins are highly conserved filamentous proteins first characterized in budding yeast and subsequently identified in all eukaryotes. Septins can bind and hydrolyze GTP which is intrinsically related to their formation of septin hexamers and functional protein interactions. The human septin family is composed of 14 loci, SEPT1-SEPT14, which encodes dozens of different septin proteins. Their central GTPase and polybasic domain regions are highly conserved but they diverge in their N-terminus and/or C-terminus. The mechanism by which the different isoforms are generated is not yet well understood, but one can hypothesize that the use of different promoters and/or alternative splicing could give rise to these variants. Septins perform diverse cellular functions according to tissue expression and their interacting partners. Functions identified to date include cell division, chromosome segregation, protein scaffolding, cellular polarity, motility, membrane dynamics, vesicle trafficking, exocytosis, apoptosis, and DNA damage response. Their expression is tightly regulated to maintain proper filament assembly and normal cellular functions. Alteration of these proteins, by mutation or expression changes, has been associated with a variety of cancers and neurological diseases. The association of septins with cancer results from expression alterations in solid tumorsor translocations in leukemias (MLL). Expression changes in septins have also been associated with neurological conditions such as Alzheimer’s and Parkinson’s disease, as well as retinopathies, Hepatitis C, spermatogenesis and Listeria infection. Pathogenic mutations of SEPT9 were identified in the autosomal dominant neurological disorder Hereditary Neuralgic Amyotrophy (HNA). Human septin research over the past decade has established their importance in cell biology and human disease. Further functional characterization of septins is crucial to our understanding of their possible diagnostic, prognostic, and therapeutic applications.

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“…Higher expression of Olfactory receptor, SEPTIN5 in transgenic cattle may strengthen the function of olfactory sense, visual sense respectively [ 76 , 77 ]. Suh M had demonstrated that fat-1 mice enriched highly ω-3 fatty acids in the retina lead to supernormal scotopic and photopic ERGs and increases in Muller cell reactivity and oxidative stress in photoreceptors [ 78 ].…”

Section: Results and Analysismentioning

confidence: 99%

Fat-1 transgenic cattle as a model to study the function of ω-3 fatty acids

et al. 2011

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ω-3 polyunsaturated fatty acids have been shown to play an important role in health. Enriched with ω-3 polyunsaturated fatty acids modulate expression of a number of genes with such broad functions as cell proliferation, growth and apoptosis and cell signaling and transduction, these effects, seem to regulate coronary artery disease, hypertension, atherosclerosis, psychiatric disorders and various cancer. In this context, fat-1 transgenic cattle was designed to convert ω-6 to ω-3 fatty acids could form an ideal model to study the effect of ω-3 fatty acids on the above functions. This study focuses on the total genomic difference of gene expression between fat-1 transgenic cattle and wild-type using cDNA microarrays, several genes were found to be overexpressed or suppressed in transgenic cattle relative to wild-type, these discrepancy genes related with lipid metabolism, immunity, inflammation nervous development and fertility.

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Septin Expression in Proliferative Retinal Membranes

Cited by 4 publications

References 29 publications

Expression of sept3, sept5a and sept5b in the Developing and Adult Nervous System of the Zebrafish (Danio rerio)

Expression of sept3, sept5a and sept5b in the Developing and Adult Nervous System of the Zebrafish (Danio rerio)

Conquering the complex world of human septins: implications for health and disease

Fat-1 transgenic cattle as a model to study the function of ω-3 fatty acids

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