2008
DOI: 10.1128/ec.00085-08
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Septins Stabilize Mitochondria in Tetrahymena thermophila

Abstract: We describe phylogenetic and functional studies of three septins in the free-living ciliate Tetrahymena thermophila. Both deletion and overproduction of septins led to vacuolization of mitochondria, destabilization of the nuclear envelope, and increased autophagy. All three green fluorescent protein-tagged septins localized to mitochondria. Specific septins localized to the outer mitochondrial membrane, to septa formed during mitochondrial scission, or to the mitochondrion-associated endoplasmic reticulum. The… Show more

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Cited by 46 publications
(76 citation statements)
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“…In contrast to the experiments with other eukaryotic cells, in TP, we did not observe mitochondria with bright fluorescence. The weak intensity of Mito Tracker Red fluorescence in TP mitochondria was also reported by other authors [27].…”
Section: Tpyriformissupporting
confidence: 86%
“…In contrast to the experiments with other eukaryotic cells, in TP, we did not observe mitochondria with bright fluorescence. The weak intensity of Mito Tracker Red fluorescence in TP mitochondria was also reported by other authors [27].…”
Section: Tpyriformissupporting
confidence: 86%
“…An involvement of autophagic-like processes in possible turnover or remodeling of metabolic organelles (mitochondria, chloroplasts) was revealed by studies with C. reinhardtii mutants deficient for chlorophyll production (due to the absence of phytoene synthase), 95 and Tetrahymena thermophila mutants in which mitochondrial function and fission were compromised by either overexpression or deletion of septin. 96 In the Tetrahymena mutants, far higher numbers of mitochondria were seen in association with autophagosome-like vesicles than in wild-type or starved ciliates. Following treatment with trans-resveratrol, inclusion of mitochondria within autophagosome-like structures was also seen in another ciliate species, Philasterides dicentrarchi.…”
Section: Functional Analyses Of Autophagy In Parasitic Protistsmentioning
confidence: 94%
“…Septins have been functionally linked to mitochondrial morphology in the protist Tetrahymena thermophila, where overexpression of the GFP-tagged septin Sep1 disrupted mitochondrial organization in some cells and led to the apparent autophagy of mitochondria (42). Sep1-GFP localizes to the cortical rows of mitochondria positioned adjacent to each motile cilium (42) (Fig.…”
Section: Loss Of Bud4 Function Sensitizes Yeast Cells To Nonlethal Inmentioning
confidence: 99%