Septoria tritici blotch resistance gene<i>Stb15</i>encodes a lectin receptor-like kinase

Hafeez, Amber N.; Chartrain, Laetitia; Feng, Cong; Cambon, Florence; Clarke, Martha; Griffiths, Simon; Hayta, Sadiye; Jiang, Mei; Keller, Beat; Kirby, Rachel; Kolodziej, Markus C.; Powell, Oliver R.; Smedley, Mark; Steuernagel, Burkhard; Xian, Wenfei; Wingen, Luzie U.; Cheng, Shifeng; Saintenac, Cyrille; Wulff, Brande B. H.; Brown, James K. M.

doi:10.1101/2023.09.11.557217

Cited by 8 publications

(3 citation statements)

References 74 publications

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“…This nding illustrated that our GHA approach identi es genes involved in host specialization. We note that the Avr Stb6 locus was not signi cantly associated with any cultivar even though we included Arina, which is known to carry the Stb6 and Stb15 resistance genes 17,19 . As our approach relies on an allelic enrichment on a particular host, this suggests the Stb6 resistance gene is widely distributed across our panel of wheat cultivars, as already shown for many wheat varieties and landraces 5,38 .…”

Section: Resultsmentioning

confidence: 99%

“…Z. tritici populations harbor all of the characteristics that facilitate rapid adaptation, including large effective population sizes, short generation times, high mutation rates, and extensive gene ow among populations that encompass international agricultural landscapes 16 . Despite the importance of Z. tritici in agroecosystems, the majority of the avirulence genes associated with the 21 major isolate-speci c STB resistance genes remain unknown, though three avirulence genes have been identi ed 5,11,[17][18][19][20][21] . During the asymptomatic phase of its hemibiotrophic lifestyle, Z. tritici suppresses activation of the host immune system 22,23 .…”

Section: Introductionmentioning

confidence: 99%

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A novel genome-wide association approach reveals wheat pathogen genes involved in host specialization

Lorrain,

Feurtey,

Alassimone

et al. 2024

Preprint

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Plant-pathogenic microbes, including the wheat fungal pathogen Zymoseptoria tritici, adapt to their host environment. In plants, genome-wide association studies (GWAS) have been extensively used to uncover the complexity of local adaptation and disease resistance. However, the application of GWAS to decipher the mechanisms underlying fungal pathogenicity and host adaptation trails far behind. Here, we established a genome-host association (GHA) approach to infer statistical associations between pathogen allele frequencies and host of origin for 832 fungal strains isolated from twelve different host cultivars during a natural field epidemic. We identified from two to twenty genes associated with specialization to the different wheat cultivars, including one known effector gene that provided a proof-of-concept for our GHA approach, as well as two new virulence-related genes that we validated with targeted gene knockouts. Our study highlights the polygenic genetic architecture of host adaptation and provides a novel application of GWAS in plant pathogens that transcends the limitations imposed by traditional phenotyping methods.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel genome-wide association approach reveals wheat pathogen genes involved in host specialization

Lorrain,

Feurtey,

Alassimone

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, breeders can deploy new arsenals of disease resistance genes to combat evolving pathogen populations driven by the changing climate. For example, here we identified new sources of resistance to aggressive yellow rust isolates (Supplementary Table 46) and the Watkins resources helped clone a novel gene for Septoria resistance 44 . We also provide novel mechanistic insights into the genetic control of wheat blast through the cloning of the first wheat gene conferring resistance to the devastating Bangladesh/Zambia MoT isolate (Fig.…”

Section: Discussionmentioning

confidence: 99%

Harnessing Landrace Diversity Empowers Wheat Breeding for Climate Resilience

Cheng,

Feng,

Wingen

et al. 2023

Preprint

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Breeding crops resilient to climate change is urgently needed to help ensure food security. A key challenge is to harness genetic diversity to optimise adaptation, yield, stress resilience and nutrition. We examined the genetic and phenotypic diversity of the A.E. Watkins landrace collection of bread wheat (Triticum aestivum), a major global cereal, through whole-genome re-sequencing (827 Watkins landraces and 208 modern cultivars) and in-depth field evaluation spanning a decade. We discovered that modern cultivars are derived from just two of the seven ancestral groups of wheat, leaving five groups as previously untapped sources for breeding. This provides access to landrace-specific functional variations using structured germplasm, genotyping and informatics resources. Employing complementary genetic populations and approaches, we identified thousands of high-resolution quantitative trait loci (QTL) and significant marker–trait associations for major traits, revealing many Watkins-unique loci that can confer superior traits in modern wheat. Furthermore, we identified and functionally verified causative genes for climate-change adaptation, nutritional enhancement and resistance to wheat blast. Finally, we assessed the phenotypic effects of 44,338 Watkins-unique haplotypes, introgressed from 143 prioritised QTL in the context of modern cultivars, bridging the gap between landrace diversity and current breeding. This study establishes a framework for systematically utilising genetic diversity in crop improvement to achieve sustainable food security.

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Harnessing landrace diversity empowers wheat breeding

Cheng,

Feng,

Wingen

et al. 2024

Nature

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Harnessing genetic diversity in major staple crops through the development of new breeding capabilities is essential to ensure food security1. Here we examined the genetic and phenotypic diversity of the A. E. Watkins landrace collection2 of bread wheat (Triticum aestivum), a major global cereal, by whole-genome re-sequencing of 827 Watkins landraces and 208 modern cultivars and in-depth field evaluation spanning a decade. We found that modern cultivars are derived from two of the seven ancestral groups of wheat and maintain very long-range haplotype integrity. The remaining five groups represent untapped genetic sources, providing access to landrace-specific alleles and haplotypes for breeding. Linkage disequilibrium-based haplotypes and association genetics analyses link Watkins genomes to the thousands of identified high-resolution quantitative trait loci and significant marker–trait associations. Using these structured germplasm, genotyping and informatics resources, we revealed many Watkins-unique beneficial haplotypes that can confer superior traits in modern wheat. Furthermore, we assessed the phenotypic effects of 44,338 Watkins-unique haplotypes, introgressed from 143 prioritized quantitative trait loci in the context of modern cultivars, bridging the gap between landrace diversity and current breeding. This study establishes a framework for systematically utilizing genetic diversity in crop improvement to achieve sustainable food security.

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Septoria tritici blotch resistance geneStb15encodes a lectin receptor-like kinase

Cited by 8 publications

References 74 publications

A novel genome-wide association approach reveals wheat pathogen genes involved in host specialization

A novel genome-wide association approach reveals wheat pathogen genes involved in host specialization

Harnessing Landrace Diversity Empowers Wheat Breeding for Climate Resilience

Harnessing landrace diversity empowers wheat breeding

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