Sequence analyses of fimbriae subunit FimA proteins on Actinomyces naeslundii genospecies 1 and 2 and Actinomyces odontolyticus with variant carbohydrate binding specificities

Drobni, Mirva; Hallberg, K.; Öhman, Ulla; Birve, Anna; Persson, Karina; Johansson, Ingegerd; Strömberg, Nicklas

doi:10.1186/1471-2180-6-43

Cited by 12 publications

(6 citation statements)

References 34 publications

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“…Notably, four loci encoding homologs of known fimbrial subunits FimA, FszB and FszD were identified in the B. dentium Bd1 genome ( Figure 9 ). FimA (BDP_0535 and BDP_1224) displays high identity to FimA homologs found in oral commensals such as A. naeslundii and A. odontolyticus [91] , [95] .…”

Section: Resultsmentioning

confidence: 99%

The Bifidobacterium dentium Bd1 Genome Sequence Reflects Its Genetic Adaptation to the Human Oral Cavity

et al. 2009

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Bifidobacteria, one of the relatively dominant components of the human intestinal microbiota, are considered one of the key groups of beneficial intestinal bacteria (probiotic bacteria). However, in addition to health-promoting taxa, the genus Bifidobacterium also includes Bifidobacterium dentium, an opportunistic cariogenic pathogen. The genetic basis for the ability of B. dentium to survive in the oral cavity and contribute to caries development is not understood. The genome of B. dentium Bd1, a strain isolated from dental caries, was sequenced to completion to uncover a single circular 2,636,368 base pair chromosome with 2,143 predicted open reading frames. Annotation of the genome sequence revealed multiple ways in which B. dentium has adapted to the oral environment through specialized nutrient acquisition, defences against antimicrobials, and gene products that increase fitness and competitiveness within the oral niche. B. dentium Bd1 was shown to metabolize a wide variety of carbohydrates, consistent with genome-based predictions, while colonization and persistence factors implicated in tissue adhesion, acid tolerance, and the metabolism of human saliva-derived compounds were also identified. Global transcriptome analysis demonstrated that many of the genes encoding these predicted traits are highly expressed under relevant physiological conditions. This is the first report to identify, through various genomic approaches, specific genetic adaptations of a Bifidobacterium taxon, Bifidobacterium dentium Bd1, to a lifestyle as a cariogenic microorganism in the oral cavity. In silico analysis and comparative genomic hybridization experiments clearly reveal a high level of genome conservation among various B. dentium strains. The data indicate that the genome of this opportunistic cariogen has evolved through a very limited number of horizontal gene acquisition events, highlighting the narrow boundaries that separate commensals from opportunistic pathogens.

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Section: Resultsmentioning

confidence: 99%

The Bifidobacterium dentium Bd1 Genome Sequence Reflects Its Genetic Adaptation to the Human Oral Cavity

et al. 2009

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“…Previously the fimA gene, the major subunit of type-2 fimbriae, was sequenced in four strains for each “ A. naeslundii genospecies 1 and 2” revealing conserved regions as well as sequence differences [9] . The fimP gene, major subunit of type-1 fimbriae, was obtained from four strains of “ A. naeslundii genospecies 2” and a human “ A. viscosus ” strain [31] .…”

Section: Discussionmentioning

confidence: 99%

“…However, diversity of fimA sequences within each genotype was apparent and strains LY7 and P-5-N clearly harboured fimA genes with sequences different to those of the other three studied in each genotype although the fimP gene from 5 A. naeslundii genospecies 2 isolates exhibited ≥98 sequence homology. Members of these two genospecies also exhibited different patterns of interaction with other bacteria, red blood cells and salivary proteins [9] . These two genospecies are early colonisers of tooth surfaces but A. naeslundii genospecies 2 is isolated from the mouth more frequently and in greater numbers than A. naeslundii genospecies 1 [5] , [10] .…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have also reported on the intra- and inter-species diversities of A. naeslundii and A. oris , as A. naeslundii genospecies 1 and 2 using DNA fingerprinting [11] , ribotyping [5] , [12] , amplified 16S ribosomal DNA restriction analysis (ARDRA) [4] , [13] , DNA probes [14] , sequence analysis of fimA genes [9] and REP-PCR amplicons analysis [10] . All these studies have demonstrated extensive heterogeneity within each taxon.…”

Section: Introductionmentioning

confidence: 99%

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Application of MLST and Pilus Gene Sequence Comparisons to Investigate the Population Structures of Actinomyces naeslundii and Actinomyces oris

Henssge

Gilbert

et al. 2011

PLoS ONE

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Actinomyces naeslundii and Actinomyces oris are members of the oral biofilm. Their identification using 16S rRNA sequencing is problematic and better achieved by comparison of metG partial sequences. A. oris is more abundant and more frequently isolated than A. naeslundii. We used a multi-locus sequence typing approach to investigate the genotypic diversity of these species and assigned A. naeslundii (n = 37) and A. oris (n = 68) isolates to 32 and 68 sequence types (ST), respectively. Neighbor-joining and ClonalFrame dendrograms derived from the concatenated partial sequences of 7 house-keeping genes identified at least 4 significant subclusters within A. oris and 3 within A. naeslundii. The strain collection we had investigated was an under-representation of the total population since at least 3 STs composed of single strains may represent discrete clusters of strains not well represented in the collection. The integrity of these sub-clusters was supported by the sequence analysis of fimP and fimA, genes coding for the type 1 and 2 fimbriae, respectively. An A. naeslundii subcluster was identified with both fimA and fimP genes and these strains were able to bind to MUC7 and statherin while all other A. naeslundii strains possessed only fimA and did not bind to statherin. An A. oris subcluster harboured a fimA gene similar to that of Actinomyces odontolyticus but no detectable fimP failed to bind significantly to either MUC7 or statherin. These data are evidence of extensive genotypic and phenotypic diversity within the species A. oris and A. naeslundii but the status of the subclusters identified here will require genome comparisons before their phylogenic position can be unequivocally established.

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“…In the past decade the fibrin-based plasticlike substances (Bioplasts) have been applied with success in clinical practice as resorbable surgical implant materials in fields of gynecology [ 11, ophthalmology [2], and particularly for the reinforcement of sutures after the excision of liver tissues [3]. This material does not cause immune response [4] and, being hydrophilic, swells well in the body fluids.…”

Section: Introductionmentioning

confidence: 99%

Modification of fibrin by synthetic polymers

Kovács¹,

Fedorova²,

Kiss³

et al. 1979

J. polym. sci., C Polym. symp.

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SYNOPSISFibrin-based resorbable surgical implant material is applied in clinical practice with success. However, its good swelling is a disadvantage in using it for fixing broken bones. To alter the swelling properties of fibrin, chemical modifications were carried out on it by synthetic polymers and polymer reactions. The following modified fibrins were prepared and served as models to study the swelling and enzymatic digestion under in v i m conditions: fibrin with pendant semimaleate groups, with poly(ethy1ene oxide) and poly(N -vinyl-2-pyrrolidinone) side chains, fibrin-copoly(ethy1ene maleic anhydride), and fibrin-copoly(ethy1ene maleic acid) polymer substances.(4) and fibrin-copoly(ethy1ene maleic acid) (5).Enzymatic investigations were carried out on the obtained products by trypsin and fibrinolysine to select the most favorable modification from the viewpoint of absorbability.

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Sequence analyses of fimbriae subunit FimA proteins on Actinomyces naeslundii genospecies 1 and 2 and Actinomyces odontolyticus with variant carbohydrate binding specificities

Cited by 12 publications

References 34 publications

The Bifidobacterium dentium Bd1 Genome Sequence Reflects Its Genetic Adaptation to the Human Oral Cavity

The Bifidobacterium dentium Bd1 Genome Sequence Reflects Its Genetic Adaptation to the Human Oral Cavity

Application of MLST and Pilus Gene Sequence Comparisons to Investigate the Population Structures of Actinomyces naeslundii and Actinomyces oris

Modification of fibrin by synthetic polymers

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