Sequence analysis and expression pattern of<i>MGTA1</i>gene in rice blast pathogen<i>Magnaporthe grisea</i>

Wang, Jiaoyu; Liu, Xiao-Hong; Lu, Jianping; Lin, Fu‐Cheng

doi:10.1631/jzus.2005.b0817

Cited by 1 publication

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“…oryzae wild-type was Guy11 (Notteghem and Silue, 1992); Δmgpex6 mutant (Wang et al, 2007) was a kind gift from Dr. Zhengyi Wang (Talbot's Laboratory, Exeter University, UK). All M. oryzae strains used were grown routinely on complete medium (CM) (Talbot et al, 1993) using routine procedures (Wang et al, 2005). Escherichia coli strain DH5α and Agrobacterium tumefaciens strain AGL1 were cultured on Luria-Bertani (LB) medium in 37 °C and 28 °C, respectively.…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

Fluorescent co-localization of PTS1 and PTS2 and its application in analysis of the gene function and the peroxisomal dynamic in Magnaporthe oryzae

Wang¹,

Zhang³

et al. 2008

J. Zhejiang Univ. Sci. B

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Abstract:The peroxisomal matrix proteins involved in many important biological metabolism pathways in eukaryotic cells are encoded by nucleal genes, synthesized in the cytoplasm and then transported into the organelles. Targeting and import of these proteins depend on their two peroxisomal targeting signals (PTS1 and PTS2) in sequence as we have known so far. The vectors of the fluorescent fusions with PTS, i.e., green fluorescence protein (GFP)-PTS1, GFP-PTS2 and red fluorescence protein (RFP)-PTS1, were constructed and introduced into Magnaporthe oryzae Guy11 cells. Transformants containing these fusions emitted fluorescence in a punctate pattern, and the locations of the red and green fluorescence overlapped exactly in RFP-PTS1 and GFP-PTS2 co-transformed strains. These data indicated that both PTS1 and PTS2 fusions were imported into peroxisomes. A probable higher efficiency of PTS1 machinery was revealed by comparing the fluorescence backgrounds in GFP-PTS1 and GFP-PTS2 transformants. By introducing both RFP-PTS1 and GFP-PTS2 into Δmgpex6 mutants, the involvement of MGPEX6 gene in both PTS1 and PTS2 pathways was proved. In addition, using these transformants, the inducement of peroxisomes and the dynamic of peroxisomal number during the pre-penetration processes were investigated as well. In summary, by the localization and co-localization of PTS1 and PTS2, we provided a useful tool to evaluate the biological roles of the peroxisomes and the related genes.

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