Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases.

Chua, Kee Chaing; Stewart, G.A.; Thomas, Wayne R.; Simpson, Richard J.; Dilworth, R.J.; Plozza, T.M.; Turner, Kati

doi:10.1084/jem.167.1.175

Cited by 466 publications

(265 citation statements)

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“…These allergens appear to be structural homologues and have very similar Nterminal amino acid sequences. The Group II allergens (Derp II and Derf II) are proteins (Me, 15 000) and also heterogeneous on IEF, with almost identical N-terminal amino acid sequences (5,11,34).a The gene encQding Derp I has been cloned and expressed in lambda gt 11 and the full amino acid sequence has been deduced from the cDNA (28). The majority of mite allergic individuals produce IgE antibody to both the Group I and Group II allergens.…”

Section: Identification and Standardizationmentioning

confidence: 99%

Dust mite allergens and asthma—A worldwide problem

Platts‐Mills¹,

deWeck²,

Aalberse³

et al. 1989

Journal of Allergy and Clinical Immunology

599

304

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After the discovery ofhouse dust mites in 1964 their association with asthma has been reported from many different parts of the world including the developing countries. Two sets of major allergens from mites of the genus Dermatophagoides are now well recognized. The Group I allergens are glycoproteins of relative molecular mass (Mr) 25 000, which show both structural homology and cross-reactivity. The allergen Der p I has been cloned and sequenced confirming the M, and establishing its nature as a protease. The Group II allergens (Mr 15 000) show even closer homology and cross-reactivity.Specific immunoassays for Group I and Group II allergens, using monospecific antisera and monoclonal antibodies, have been standardized and are suitable for measuring allergen levels in different parts of the world.Measures for reducing the levels of mite allergens in houses include the covering of mattresses, hot washing of bedding, and removal of carpets from bedrooms as well as humidity control, vacuum cleaning, and the use ofacaricides in the rest ofthe house. There is already evidence that these procedures can cause a major improvement in the symptoms of asthma. While provisional standards for both sensitization to mites and also mite allergen exposure can now be recommended, there is an urgent needfor controlled studies using protocols demonstrated to reduce mite allergen levels by at least tenfold and for further international collaboration.

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Section: Identification and Standardizationmentioning

confidence: 99%

Dust mite allergens and asthma—A worldwide problem

Platts‐Mills¹,

deWeck²,

Aalberse³

et al. 1989

Journal of Allergy and Clinical Immunology

599

304

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“…La presencia de un número de bandas mayor a las esperadas es un indicativo de la presencia de familia de genes relacionados (4,5,16). Por análisis de Southern blot y RT-PCR se determinó que los alergenos Der p 1, Der p 3 del ácaro Dermatophagoides pteronyssinus y Der f 1 del Dermatophagoides farinae están codificados cada uno por un solo gen y que sus isoformas son producto de sus respectivas variantes alélicas, por lo que el polimorfismo en estos alergenos se debe a la presencia de varios alelos (4,17,18).…”

Section: Identificación Del Polimorfismounclassified

El polimorfismo en los alergenos

2002

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Los alergenos son antígenos que inducen una respuesta inmunológica mediada por anticuerpos tipo IgE. Estos pueden provenir de diferentes fuentes como pólenes, ácaros, mohos, animales, insectos y alimentos. Una molécula alergénica consiste en un número determinado de epítopes o determinantes antigénicos. Algunos alergenos están constituidos por varias moléculas similares, las cuales presentan variaciones menores en la secuencia de aminoácidos, es decir, presentan polimorfismo y de acuerdo con el grado de similitud que presenten, se denominan isoalergenos o variantes. El polimorfismo puede ser el resultado de la presencia de varios alelos, como también de la presencia de familias de genes relacionados. Este fenómeno se ha detectado en alergenos de diferentes fuentes como los ácaros, los pólenes y las cucarachas, entre otros. El polimorfismo puede tener un efecto importante sobre los epítopes reconocidos por los linfocitos T, por los anticuerpos monoclonales y por la IgE de pacientes alérgicos, lo cual afecta la capacidad alergénica o el grado de reactividad cruzada con alergenos de otras especies. La existencia de isoformas con una capacidad nula o disminuida de unirse a la IgE, pero con capacidad de estimular los linfocitos T, se ha planteado como una alternativa atractiva en la inmunoterapia de las alergias. La estandarización de extractos alergénicos puede verse afectada por la presencia de isoformas en diferentes proporciones en fuentes alergénicas de diferentes regiones geográficas.Palabras clave: alergenos recombinantes, polimorfismo, isoalergenos, epítopes, IgE. Polymorphism in allergensAllergens are antigens that elicit an IgE-mediated immune response; they originate from diverse sources such as pollens, mites, molds, mammal exudates, insects and food. Allergenic molecules can contain several antigenic determinants, termed epitopes. Allergenic proteins have been discovered with polymorphisms, i.e., a mixture of similar molecules with minor variations in their amino acid sequences. These are called isoallergens or allergenic variants depending on the degree of similarity. Polymorphism may be defined by the presence of several alleles of the same gene or as families of related genes. Polymorphisms can have an important effect on the epitopes recognized by T lymphocytes, monoclonal antibodies and IgE of allergic patients. Individual polymorphisms can affect the basal level of allergenicity as well as the cross-reactivity with other allergens. The use of isoforms with low or total absence of IgE binding capacity but with high capacity to stimulate T cell response has been suggested as an alternative to the conventional immunotherapy for allergic diseases. Standardization of allergenic compounds can be affected by the differing proportions of isoforms in allergenic sources from different regions. En una fuente de alergenos, como los ácaros del polvo de habitación, se encuentran diversas proteínas con capacidad de unirse a la IgE. Los alergenos se caracterizan y diferencian básicamente por el tamaño molecular, e...

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“…A few examples of wellknown allergens characterized in this way are ragweed allergens Amb a 1 [1] and Amb a 2 [2], grass pollen allergens Lol p 1 [3,4] and Lol p 2 [5], house dust mite allergens Der p 1 [6] and Der f 1 [7], peanut allergen Ara h 1 [8], shrimp allergen Met e 1 [9], and latex allergen Hev b 1 [10]. This technology has the potential to be useful in several ways.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of Ragweed Allergen Amb a 6

Lubahn

1998

Scand J Immunol

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Hiller KM, Lubahn BC, Klapper DG. Cloning and Expression of Ragweed Allergen Amb a 6. Scand J Immunol 1998;48:26-36 We have cloned the protein coding region of an isoform of short ragweed allergen Amb a 6 (Ra6) and expressed the secreted product in Pichia pastoris at mg/l levels. 5 0 RACE was performed using sequence obtained from a partial Amb a 6 clone. This yielded a product whose deduced protein sequence has a characteristic signal sequence motif at the N-terminus followed by sequence consistent with that previously published for highly purified Amb a 6 [Roebber et al. J Immunol 1983;131:706-11]. The region encoding the secreted product was amplified by PCR and cloned into pPICZaa, an expression vector for the yeast Pichia pastoris. Yeast transformed with this vector secrete a protein which migrates near Amb a 6 in SDS-PAGE. This secreted protein reacts with polyclonal anti-Amb a 6 antisera as well as an anti-Amb a 6 monoclonal antibody, and has the N-terminal sequence of Amb a 6. By time-of-flight mass spectrometry, recombinant Amb a 6 has a molecular weight of 9884 Ϯ 0.2%. In addition to the deduced amino acid sequence of an Amb a 6 clone, the amino acid sequence of Amb a 6 protein is reported for comparison. The amino acid sequence was obtained by aligning overlapping tryptic and chymotryptic peptides from enzymatic digests of extensively reduced and alkylated Amb a 6. Sequences from at least three closely related Amb a 6 isoforms are present among these peptides. The amino acid sequence closely matches the deduced amino acid sequence of the Amb a 6 clone.

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Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases.

Cited by 466 publications

References 24 publications

Dust mite allergens and asthma—A worldwide problem

Dust mite allergens and asthma—A worldwide problem

El polimorfismo en los alergenos

Cloning and Expression of Ragweed Allergen Amb a 6

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