Sequence analysis of<i>Escherichia coli</i>O157:H7 bacteriophage Î¦V10 and identification of a phage-encoded immunity protein that modifies the O157 antigen

Perry, Lynda; SanMiguel, Phillip; Minocha, Udit; Terekhov, Anton I.; Shroyer, Mindy; Farris, Leigh; Bright, Nathan G.; Reuhs, Bradley L.; Applegate, Bruce

doi:10.1111/j.1574-6968.2009.01511.x

Cited by 62 publications

(49 citation statements)

References 21 publications

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“…Therefore, AR1 phage has the potential to be used as a bactericidal food additive to prevent contamination with E. coli O157:H7 in the food industry. In addition, several reports have indicated that some antibiotics may increase Shiga-like toxin production, resulting in increased side effects for patients (39,61). Therefore, AR1 could also be used as an alternative therapeutic agent for treating E. coli O157:H7 infection.…”

Section: Discussionmentioning

confidence: 99%

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T4-Like Genome Organization of the Escherichia coli O157:H7 Lytic Phage AR1

Liao¹,

Ng²,

Lin³

et al. 2011

J Virol

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We report the genome organization and analysis of the first completely sequenced T4-like phage, AR1, of Escherichia coli O157:H7. Unlike most of the other sequenced phages of O157:H7, which belong to the temperate Podoviridae and Siphoviridae families, AR1 is a T4-like phage known to efficiently infect this pathogenic bacterial strain. The 167,435-bp AR1 genome is currently the largest among all the sequenced E. coli O157:H7 phages. It carries a total of 281 potential open reading frames (ORFs) and 10 putative tRNA genes. Of these, 126 predicted proteins could be classified into six viral orthologous group categories, with at least 18 proteins of the structural protein category having been detected by tandem mass spectrometry. Comparative genomic analysis of AR1 and four other completely sequenced T4-like genomes (RB32, RB69, T4, and JS98) indicated that they share a well-organized and highly conserved core genome, particularly in the regions encoding DNA replication and virion structural proteins. The major diverse features between these phages include the modules of distal tail fibers and the types and numbers of internal proteins, tRNA genes, and mobile elements. Codon usage analysis suggested that the presence of AR1-encoded tRNAs may be relevant to the codon usage of structural proteins. Furthermore, protein sequence analysis of AR1 gp37, a potential receptor binding protein, indicated that eight residues in the C terminus are unique to O157:H7 T4-like phages AR1 and PP01. These residues are known to be located in the T4 receptor recognition domain, and they may contribute to specificity for adsorption to the O157:H7 strain.

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Section: Discussionmentioning

confidence: 99%

“…NC_011357; Y. Zhang et al, unpublished data); the enterobacterial phages 933W (64), VT2-Sakai (40,47), Min27 (36,77), and YYZ-2008 (GenBank accession no. NC_011356; Zhang et al, unpublished data), which encode the Stx-like proteins; and the nontoxin-carrying phages phiV10 (61) and JK06 (GenBank accession no. NC_007291; J. Kagan et al, unpublished data).…”

mentioning

confidence: 99%

T4-Like Genome Organization of the Escherichia coli O157:H7 Lytic Phage AR1

Liao¹,

Ng²,

Lin³

et al. 2011

J Virol

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“…A contig of 39 kb shared similarity with the epsilon15-like Podovirus phiV10 over 63% of its genome length covering virion, lysis, lysogeny and replication genes (Perry et al, 2009) (Fig. 4C).…”

Section: Rtpmentioning

confidence: 98%

Safety analysis of a Russian phage cocktail: From MetaGenomic analysis to oral application in healthy human subjects

et al. 2013

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Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli/Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30 kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure.

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“…In the case of all three homologous phages, it is the morphogenic genes which are conserved. Perry and Applegate sequenced the E. coli O157:H7-specifi c phage V10 [ 139 ], misnamed φV10, and showed that it displays considerable sequence similarity to ε15, but lacks the conversion genes of this phage [ 140 ].…”

Section: Epsilon15likevirusmentioning

confidence: 99%

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Switt

Sulakvelidze

Wiedmann

et al. 2014

Methods in Molecular Biology

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Since this book was originally published in 2007 there has been a significant increase in the number of Salmonella bacteriophages, particularly lytic virus, and Salmonella strains which have been fully sequenced. In addition, new insights into phage taxonomy have resulted in new phage genera, some of which have been recognized by the International Committee of Taxonomy of Viruses (ICTV). The properties of each of these genera are discussed, along with the role of phage as agents of genetic exchange, as therapeutic agents, and their involvement in phage typing.

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Sequence analysis ofEscherichia coliO157:H7 bacteriophage Î¦V10 and identification of a phage-encoded immunity protein that modifies the O157 antigen

Cited by 62 publications

References 21 publications

T4-Like Genome Organization of the Escherichia coli O157:H7 Lytic Phage AR1

T4-Like Genome Organization of the Escherichia coli O157:H7 Lytic Phage AR1

Safety analysis of a Russian phage cocktail: From MetaGenomic analysis to oral application in healthy human subjects

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

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