Sequence analysis of infectious pancreatic necrosis virus isolated from Iranian reared rainbow trout (Oncorhynchus mykiss) in 2012

Dadar, Maryam; Peyghan, Rahim; Memari, Hamid Rajabi; Shapouri, M.R Seifi Abad; Hasanzadeh, Reza; Goudarzi, Laleh Moazzami; Vakharia, Vikram N.

doi:10.1007/s11262-013-0981-4

Cited by 24 publications

(21 citation statements)

References 39 publications

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“…This is particularly evident between the Chilean and the North American isolates (WB and DM) within genotype 4, where the clustering of Chilean isolates could suggest the formation of a fifth genotype. Similar tradeassociated transmissions of IPNV have been suggested for isolates from Spain, Japan, Iran, and Ireland, that show a great similarity with reference strains from the sources where these countries have imported salmonid fish and eggs (Cutrín et al 2004, Nishizawa et al 2005, Ruane et al 2009, 2015, Dadar et al 2013. Concordantly, the phylogenetic trees in these studies also showed grouping among the local isolates and the formation of minor clusters within genogroups.…”

Section: Discussionsupporting

confidence: 77%

Detection and phylogenetic analysis of infectious pancreatic necrosis virus in Chile

Tapia¹,

Eissler²,

Torres³

et al. 2015

Dis. Aquat. Org.

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Infectious pancreatic necrosis virus (IPNV) is the etiological agent of a highly contagious disease that is endemic to salmon farming in Chile and causes great economic losses to the industry. Here we compared different diagnostic methods to detect IPNV in field samples, including 3 real-time reverse transcription PCR (qRT-PCR) assays, cell culture isolation, and indirect fluorescent antibody test (IFAT). Additionally, we performed a phylogenetic analysis to investigate the genogroups prevailing in Chile, as well as their geographic distribution and virulence. The 3 qRT-PCR assays used primers that targeted regions of the VP2 and VP1 genes of the virus and were tested in 46 samples, presenting a fair agreement within their results. All samples were positive for at least 2 of the qRT-PCR assays, 29 were positive for cell culture, and 23 for IFAT, showing less sensitivity for these latter 2 methods. For the phylogenetic analysis, portions of 1180 and 523 bp of the VP2 region of segment A were amplified by RT-PCR, sequenced and compared with sequences from reference strains and from isolates reported by previous studies carried out in Chile. Most of the sequenced isolates belonged to genogroup 5 (European origin), and 5 were classified within genogroup 1 (American origin). Chilean isolates formed clusters within each of the genogroups found, evidencing a clear differentiation from the reference strains. To our knowledge, this is the most extensive study completed for IPNV in Chile, covering isolates from sea-and freshwater salmon farms and showing a high prevalence of this virus in the country.

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Section: Discussionsupporting

confidence: 77%

Detection and phylogenetic analysis of infectious pancreatic necrosis virus in Chile

Tapia¹,

Eissler²,

Torres³

et al. 2015

Dis. Aquat. Org.

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“…IPNV was identified in rainbow trout fry in 2002 (Candan, ), and several reports have described the presence of IPNV in different regions of Turkey (Gurcay, Turan, & Parmaksiz, ; Kalayci, Incioglu, Özyer, & Küçükalİ, ; Ogut & Altuntas, ; Ogut, Altuntas, & Parlak, ). The presence of IPNV in neighbouring Iran was also described recently (Dadar et al., , ; Soltani et al., ). However, the prevalence, genetic and epidemiological relationship between IPNV from farms in different parts of Turkey have not been investigated.…”

Section: Introductionsupporting

confidence: 53%

Infectious pancreatic necrosis virus (IPNV) serotype Sp is prevalent in Turkish rainbow trout farms

Büyükekiz

Altun

Hansen

et al. 2017

Journal of Fish Diseases

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Infectious pancreatic necrosis virus (IPNV) is a common pathogen of rainbow trout (Oncorhynchus mykiss) in Turkey. We found that 455 of 1,676 sample pools tested were IPNV positive. Positive samples were found in all geographical regions where sampling was conducted. Sequence and phylogenetic analyses of VP2 from 30 isolates representing all regions showed that the viruses were highly similar in sequence and grouped within Genogroup 5 (serotype Sp-A2). No correlations between sequences, sampling sites or geographical origins were identified. Although clinical disease was evident in several farms, analyses of the amino acid sequence of VP2 showed that all virus strains harboured the P 217 T 221 motif, assumed to be associated with low virulence. We conclude that IPNV is prevalent in Turkish rainbow trout farms and that the viruses are very homogenous and likely to be of European origin. Frequent exchange of eggs and live fish within the farming industry may explain the homogeneity of the IPNV.

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“…Moving on to Asia, Iranian Sp IPNV isolates obtained from cultured rainbow trout were demonstrated to have the Pro 217 -Thr 221 -Ala 247 profile [101] ( Figure 6K), which disagrees with Song et al [95]. In Mexico [102], IPNV isolates showed Thr at position 221 ( Figure 6L) which is indicative of persistence; however, although most isolates were from asymptomatic carriers-which confirms the predictions-one corresponded to an isolation from diseased rainbow trout.…”

Section: Determinants Of Virulence In Vp2mentioning

confidence: 68%

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

Dopazo

2020

Pathogens

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Infectious pancreatic necrosis (IPN) is a disease of great concern in aquaculture, mainly among salmonid farmers, since losses in salmonid fish—mostly very young rainbow trout (Salmo gairdnery) fry and Atlantic salmon (Salmo salar) post-smolt—frequently reach 80–90% of stocks. The virus causing the typical signs of the IPN disease in salmonids, named infectious pancreatic necrosis virus (IPNV), has also been isolated from other fish species either suffering related diseases (then named IPNV-like virus) or asymptomatic; the general term aquabirnavirus is used to encompass all these viruses. Aquabirnaviruses are non-enveloped, icosahedral bisegmented dsRNA viruses, whose genome codifies five viral proteins, three of which are structural, and one of them is an RNA-dependent RNA polymerase. Due to the great importance of the disease, there have been great efforts to find a way to predict the level of virulence of IPNV isolates. The viral genome and proteins have been the main focus of research. However, to date such a reliable magic marker has not been discovered. This review describes the processes followed for decades in the attempts to discover the viral determinants of virulence, and to help the reader understand how viral components can be involved in virulence modulation in vitro and in vivo. There is also a brief description of the disease, of host defenses, and of the molecular structure and function of the virus and its viral components.

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Sequence analysis of infectious pancreatic necrosis virus isolated from Iranian reared rainbow trout (Oncorhynchus mykiss) in 2012

Cited by 24 publications

References 39 publications

Detection and phylogenetic analysis of infectious pancreatic necrosis virus in Chile

Detection and phylogenetic analysis of infectious pancreatic necrosis virus in Chile

Infectious pancreatic necrosis virus (IPNV) serotype Sp is prevalent in Turkish rainbow trout farms

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

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