2001
DOI: 10.1128/aem.67.5.2062-2069.2001
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Sequence Analysis of Insecticidal Genes from Xenorhabdus nematophilus PMFI296

Abstract: Three strains of Xenorhabdus nematophilus showed insecticidal activity when fed to Pieris brassicae (cabbage white butterfly) larvae. From one of these strains (X. nematophilus PMFI296) a cosmid genome library was prepared in Escherichia coli and screened for oral insecticidal activity. Two overlapping cosmid clones were shown to encode insecticidal proteins, which had activity when expressed in E. coli (50% lethal concentration [LC 50 ] of 2 to 6 g of total protein/g of diet). The complete sequence of one co… Show more

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Cited by 132 publications
(93 citation statements)
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“…P. luminescens bacteria are known to produce insecticidal proteins (Bowen and Ensign, 1998;ffrench-Constant and Bowen, 2000;Au et al, 2004) and an extracellular protease has been suggested to participate in activation of the toxin complex (Bowen et al, 1999). X. nematophila bacteria also express insecticidal proteins (Morgan et al, 2001) and secrete proteases which are known to be active against insect immune factors (Caldas et al, 2002); however, this and our previous study (Bussaman et al, 2006) are the first to report the miticidal activity of Photorhabdus and Xenorhabdus 297 Symbiotic Bacteria against the Mushroom Mite bacteria. Our results also show that bacterial culture of the X1 strain of X. nematophila is more effective than the previously tested strains Thai and All.…”
Section: Discussionmentioning
confidence: 99%
“…P. luminescens bacteria are known to produce insecticidal proteins (Bowen and Ensign, 1998;ffrench-Constant and Bowen, 2000;Au et al, 2004) and an extracellular protease has been suggested to participate in activation of the toxin complex (Bowen et al, 1999). X. nematophila bacteria also express insecticidal proteins (Morgan et al, 2001) and secrete proteases which are known to be active against insect immune factors (Caldas et al, 2002); however, this and our previous study (Bussaman et al, 2006) are the first to report the miticidal activity of Photorhabdus and Xenorhabdus 297 Symbiotic Bacteria against the Mushroom Mite bacteria. Our results also show that bacterial culture of the X1 strain of X. nematophila is more effective than the previously tested strains Thai and All.…”
Section: Discussionmentioning
confidence: 99%
“…In some cases, expression of individual tcA genes is sufficient to cause toxicity (8,11), but full toxicity generally requires all three Tc components, with the B and C components providing a potentiation of toxicity (12,13). Interestingly, TcB and TcC components coexpressed with TcA components from other species (or from other Tc clusters) can combine to cause an effect with altered host specificity (12,13), suggesting the TcA component is a determinant of target host range.…”
mentioning
confidence: 99%
“…Subsequent to their initial discovery, the Tc classes have been redesignated A, B, and C, and the P. luminescens Tc proteins can thus be classified as either TcA-like (TcaA1, TcaB1, TcbA, TccA and TcdA1), TcB-like (TcaC1 and TcdB1), or TcC-like (TccC1 and TccC2) (6). Genes encoding Tc-like proteins have subsequently been identified in many insect-active bacteria including Serratia entomophila (tcA, sepA; tcB sepB; tcC sepC) (7) and the nematode-associated bacterium Xenorhabdus nematophila (tcA, xptA1, xptA2; tcB, xptC1; tcC, xptB1) (8), while the completion of bacterial genome sequencing projects such as Yersinia pestis C092 (9) and Pseudomonas syringae pv. tomato DC3000 (10) has revealed the presence of additional putative insecticidal tc genes.…”
mentioning
confidence: 99%
“…The high molecular mass complex consists of about 10 polypeptides, ranging in size from 30 to 200 kDa, which is secreted from the bacteria and requires proteolytic processing for activity. Multiple tc genes have been identified in several Photorhabdus species (5,36,37), and tc-like genes have been identified in other insect-associated bacteria (X. nematophila (36,38), Serratia entomophila (39), and Y. pestis (36)), as well as in Pseudomonas syringae pv. tomato, Fibrobacter succinogenes, and Treponema denticola (40).…”
Section: Discussionmentioning
confidence: 99%