Sequence Analysis of Porcine Adenovirus Type 3 E1 Region, pIX and pIVa2 Genes, and Two Novel Open Reading Frames

Aggarwal, Neeraj; Mittal, Suresh K.

doi:10.1159/000025016

Cited by 6 publications

(4 citation statements)

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“…The family Adenoviridae is comprised of five genera: Mastadenovirus, Aviadenovirus, Atadenovirus, Siadenovirus and Ichtadenovirus (Houng et al, 2006;Kovács and Benkö, 2011;Kovács et al, 2010;Lehmkuhl and Hobbs, 2008;Wellehan et al, 2004), which infect a wide range of vertebrate species (Davison et al, 2003;Morrison et al, 1997). Mastadenovirus has been identified in mammals, including human, sea lion, canine, bovine, porcine, murine and bat (Aggarwal and Mittal, 2000;Goldstein et al, 2011;Klempa et al, 2009;Kovács et al, 2004;Li et al, 2010;Morrison et al, 1997;Rusvai et al, 2000). Aviadenovirus contains falcon and other fowl adenoviruses (Davison et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Full genome analysis of a novel adenovirus from the South Polar skua (Catharacta maccormicki) in Antarctica

Park

Kim

et al. 2012

Virology

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Adenoviruses have been identified in humans and a wide range of vertebrate animals, but not previously from the polar region. Here, we report the entire 26,340-bp genome of a novel adenovirus, detected by PCR, in tissues of six of nine South Polar skuas (Catharacta maccormicki), collected in Lake King Sejong, King George Island, Antarctica, from 2007 to 2009. The DNA polymerase, penton base, hexon and fiber genes of the South Polar skua adenovirus (SPSAdV) exhibited 68.3%, 75.4%, 74.9% and 48.0% nucleotide sequence similarity with their counterparts in turkey hemorrhagic enteritis virus. Phylogenetic analysis based on the entire genome revealed that SPSAdV belonged to the genus Siadenovirus, family Adenoviridae. This is the first evidence of a novel adenovirus, SPSAdV, from a large polar seabird (family Stercorariidae) in Antarctica.

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Section: Introductionmentioning

confidence: 99%

Full genome analysis of a novel adenovirus from the South Polar skua (Catharacta maccormicki) in Antarctica

Park

Kim

et al. 2012

Virology

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“…Published reports suggest that various human adenoviruses possess the IX gene (Chroboczek et al, 1992;Dijkema et al, 1981;Engler, 1981;Mei et al, 2003). Although ovine and avian adenoviruses do not appear to express pIX (Hess et al, 1997;Ojkic & Nagy, 2000;Vrati et al, 1996), analyses of some canine, bovine and porcine adenovirus genomes have shown the presence of the IX gene (Aggarwal & Mittal, 2000;Salmon & Haj-Ahmad, 1994;Shibata et al, 1989;Zheng et al, 1999). Of note, our CAV2 vector of interest does express pIX.…”

Section: Introductionmentioning

confidence: 68%

Fluorescently tagged canine adenovirus via modification with protein IX–enhanced green fluorescent protein

Ternovoi

et al. 2005

Journal of General Virology

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Canine adenovirus type 2 (CAV2) has become an attractive vector for gene therapy because of its non-pathogenicity and the lack of pre-existing neutralizing antibodies against this virus in the human population. Additionally, this vector has been proposed as a conditionally replicative adenovirus agent under the control of an osteocalcin promoter for evaluation in a syngeneic, immunocompetent canine model with spontaneous osteosarcoma. In this study, a CAV2 vector labelled with the fluorescent capsid fusion protein IX-enhanced green fluorescent protein (pIX-EGFP) was developed. Expression of the fluorescent fusion-protein label in infected cells with proper nuclear localization, and incorporation into virions, could be detected. The labelled virions could be visualized by fluorescence microscopy; this was applicable to the tracking of CAV2 infection, as well as localizing the distribution of the vector in tissues. Expression of pIX-EGFP could be exploited to detect the replication and spread of CAV2. These results indicate that pIX can serve as a platform for incorporation of heterologous proteins in the context of a canine adenovirus xenotype. It is believed that capsid-labelled CAV2 has utility for vector-development studies and for monitoring CAV2-based oncolytic adenovirus replication. INTRODUCTIONAdenoviral vectors have been widely implemented to deliver genes for the purpose of therapy (Curiel & Douglas, 2002). The attractiveness of this vector system includes the well-documented knowledge encompassing its biology and pathology (Shenk, 1996), relative safety associated with the virus and ease in upscaling vector production. More recently, manipulation of the viral genome by recombinant techniques has yielded cell-specific vectors founded on various concepts, including transductional (Wickham, 2003), transcriptional (Miller & Whelan, 1997;Nettelbeck et al., 2000) and translational (Ahmed et al., 2003) targeting. Furthermore, enhanced safety, long-term transgene expression and greater cloning capacity have resulted from the ability to generate helper-dependent adenoviral vectors that are completely devoid of native viral genes (Kochanek et al., 2001). Despite these advances in adenoviral vector engineering, two key unresolved issues remain. First, preexisting humoral and cellular immunity against human adenoviruses, especially serotypes 2 and 5 (Ad2 and Ad5) on which most current adenoviral vectors are based, can have profound effects on initial vector administration and, increasingly, on repeat applications (Crystal et al., 1995;Zabner et al., 1994). Second, replication-competent adenovirus contamination of recombinant viral stocks poses a health risk, even in the case of gutless vectors (Lochmuller et al., 1994).Vector systems based on other, less pathogenic human adenovirus serotypes and various xenotypes have been proposed to overcome these concerns (Both, 2004;Gao et al., 2003; Rasmussen et al., 1999;Reddy et al., 1999). Among the diverse approaches, a canine adenovirus type 2 (CAV2) vector demonstrated...

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“…1). For the xenogenic mastadenoviruses there are three ORFs at the left end that show homology with HAdVs [77][78][79][80]. The genome packaging signal is also present within the first ^500 nucleotides of the HAdV5 genome [81], but until recently this had not been defined for any xenogenic virus.…”

Section: Left End Sequencesmentioning

confidence: 99%

Xenogenic Adenoviral Vectors

Both

2002

Adenoviral Vectors for Gene Therapy

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Sequence Analysis of Porcine Adenovirus Type 3 E1 Region, pIX and pIVa2 Genes, and Two Novel Open Reading Frames

Cited by 6 publications

References 50 publications

Full genome analysis of a novel adenovirus from the South Polar skua (Catharacta maccormicki) in Antarctica

Full genome analysis of a novel adenovirus from the South Polar skua (Catharacta maccormicki) in Antarctica

Fluorescently tagged canine adenovirus via modification with protein IX–enhanced green fluorescent protein

Xenogenic Adenoviral Vectors

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