Sequence Analysis of Ribosomal and Mitochondrial Genes of the Giant Liver Fluke Fascioloides magna (Trematoda: Fasciolidae): Intraspecific Variation and Differentiation from Fasciola hepatica

Králová-Hromadová, Ivica; Špakulová, Marta; Horáčková, Eva; Turčeková, Ľ.; Novobilský, Adam; Beck, Relja; Koudela, Břetislav; Tran, Albert; Rajský, D.; Pybus, M. J.

doi:10.1645/ge-1324.1

Cited by 70 publications

(65 citation statements)

References 28 publications

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“…The parasites were identified based on the sequence of the internal transcribed region 2 (ITS-2) with the use of primers designed by Wobeser and Schumann (23), Kralova-Hromadova et al (9), and Bazsalovicsova et al (2). A PCR method was used, as described by Kasny et al (7) with some modifications concerning reaction mixture and the time of amplification conditions.…”

Section: Methodsmentioning

confidence: 99%

First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland – pathomorphological changes and molecular identification

Karamon

Larska

Jasik

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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A 3-year-old female fallow deer was subjected to the necropsy and virological testing, due to a suspected infectious disease in the herd of farmed deer in the Southeastern region of Poland. The animal was found negative for the presence of BVDV, BoHV-1, BTV, and EHDV antibodies and BVDV antigen. The toxicological examination did not reveal any coccidiostats, mycotoxins, rodenticides, carbamate pesticides, and organophosphorus pesticides. The flukes found during postmortem examination were first characterised microscopically as Fascioloides magna and later their identity was confirmed by PCR and sequencing. The autopsy revealed lesions characteristic for F. magna infection, including different size cystic spaces in the liver, filled with brownish mucous fluid and flukes, and black pigment covering the surface of parietal and visceral peritoneum with the highest concentrations localised next to the liver. The changes observed in the liver tissue were typical of liver cirrhosis. The results demonstrated that in Poland, where the cervid farming is developing dynamically, the problem of fascioloidosis is present and may probably exert a significantly negative influence on the productivity of such farms if no antiparasitic treatment is performed.

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Section: Methodsmentioning

confidence: 99%

First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland – pathomorphological changes and molecular identification

Karamon

Larska

Jasik

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…The reverse primer used was trem.coxl.rrnl (S'AATCATGATGCAAAAGGTA-S') of Králová-Hromadová et al (2001).…”

Section: Methodsmentioning

confidence: 99%

Trematode parasites of Otago Harbour (New Zealand) soft‐sediment intertidal ecosystems: Life cycles, ecological roles and DNA barcodes

Leung

Donald

Keeney

et al. 2009

New Zealand Journal of Marine and Freshwater Research

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Parasites, in particular trematodes (Platyhelminthes: Digenea), play major roles in the population dynamics and community structure of invertebrates on soft-sediment mudflats. Here, we provide a list of the 20 trematode species currently known to infect molluscs, crustaceans and polychaetes from Otago Harbour (New Zealand) soft-sediment intertidal areas, as well as information on their transmission modes, life cycles, andknown ecological impacts. Several of the host-parasite species combinations recorded here are reported for the first time. We also provide DNA barcodes, based on sequences of the cytochrome oxidase subunit l (CO1) gene, for 19 of the 20 trematode species, to facilitate future identification of these parasites in marine ecological studies.

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“…We therefore compared the gene sequences of Austrian F. magna with data from the literature/gene bank. Two Austrian F. magna isolates could be identified below the species level according to the haplotype system based on sequence data of the cytochrome c oxidase subunit I ( cox1 ) and the nicotinamide dehydrogenase subunit I ( nad1 ), respectively [24, 39]. Strain FmA_R was an adult worm isolated from a red deer from Fischamend, southern shore of the Danube; strain FmA _86 was a redia isolated from a Galba truncatula from the locality Entenhaufen (near Orth), northern shore of the Danube.…”

Section: On the Origin Of F Magna Populations In Austriamentioning

confidence: 99%

Wherefrom and whereabouts of an alien: the American liver fluke Fascioloides magna in Austria: an overview

Sattmann

Hörweg

Gaub

et al. 2014

Wien Klin Wochenschr

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SummaryThe giant liver fluke Fascioloides magna, an invasive species originating from North America, was recorded in Austria in the wild for the first time in 2000. Since then, various data concerning the epidemiology in snail intermediate hosts and cervid final hosts have been reported. Galba truncatula acts as snail intermediate host, and red deer, roe deer and fallow deer act as final hosts. G. truncatula is abundant throughout the region, especially along muddy shores of slow-flowing branches of the river system. Prevalence in deer (20–100 %) is much higher than in snails (0.03–0.2 %). Despite medical treatment of parts of the deer population, the parasite has successfully established itself on both sides of the Danube floodplain environments southeast of Vienna. Genetic analysis revealed that the infection of Austrian deer populations apparently originated from foci in the Czech Republic or from populations of Danube tributaries. Areas adjacent southwards, which will soon be joined through wildlife crossings, have not yet evidenced F. magna. Nonetheless, these environments are inhabited by host snails and deer and therefore constitute suitable habitats for F. magna. Invading alien parasites not only threaten native individual hosts but also influence host populations, thus potentially also modifying parasite communities and interactions. The host range of F. magna includes a variety of potential hosts, notably other Lymnaeidae as potential intermediate hosts and various ungulates, including sheep and cattle, as final hosts. Because eradication after medical treatment was unsuccessful, and due to the risk of further spread of the parasite into unaffected regions, enhanced control strategies need to be developed. We recommend assessment of introduction pathways and dispersal, continuous monitoring of host abundance and distribution and the prevalence of flukes in intermediate and final hosts, as well as coordinated and concerted actions with neighbouring countries. This strategy could help to reduce potential negative impacts of this and other invasive parasites on host populations in Europe.

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Sequence Analysis of Ribosomal and Mitochondrial Genes of the Giant Liver Fluke Fascioloides magna (Trematoda: Fasciolidae): Intraspecific Variation and Differentiation from Fasciola hepatica

Cited by 70 publications

References 28 publications

First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland – pathomorphological changes and molecular identification

First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland – pathomorphological changes and molecular identification

Trematode parasites of Otago Harbour (New Zealand) soft‐sediment intertidal ecosystems: Life cycles, ecological roles and DNA barcodes

Wherefrom and whereabouts of an alien: the American liver fluke Fascioloides magna in Austria: an overview

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