Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85C Proteins from Clinical Isolates of Mycobacterium tuberculosis

Mertaniasih, Ni Made; Handijatno, Didik; Perwitasari, Agnes Dwi Sis; Dewi, Desak Nyoman Surya Suameitria; Fanani, Much Zaenal; Afifah, Ika Qurrotul

doi:10.1016/j.proche.2016.01.035

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…However, the results of the epitopes in this study showed different result with those reported by Mertaniasih et al (2016) which acquired the epitope T cell prediction by IAd Pattern Position for only four epitopes. This is possible because the target gene captured in this study is much longer than that done by previous studies that only got 351 bp.…”

Section: Discussioncontrasting

confidence: 99%

“…Previous research analyzing ESAT-6, Ag85B, and Ag85C sequence was in agreement with this research. In another study, mutation was also not found in esxA gene observed from 38 patients with MDR-TB (Mertaniasih et al, 2016;Uplekar et al, 2011). Another study also reported that there is no variation found in esxA gene from 88 clinical isolates (Davilla et al, 2010).…”

Section: Discussionmentioning

confidence: 87%

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T Cell Epitopes of the Esxa Full Gene of Mycobacterium Tuberculosis From Sputum of MDR-Tb Patients

Dewi

Soedarsono

Mertaniasih

2018

AJID

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Background:In 2015, World Health Organization (WHO) discovered 10.4 million tuberculosis (TB) cases around the world. Multidrug-resistant tuberculosis (MDR-TB) became a threat because it has high mortality number. There were 480,000 new MDR-TB cases in 2015. Based on those problems, diagnostic development to detect M. tuberculosis rapidly and accurately is needed. The importance of detecting epitope expression of esxA full gene because there was a potential of complexity over the protein structure and might affect the protein concentration. By knowing epitope prediction, there’s an expectation that it can help the development of TB diagnostic. This research was aimed to determine the T cell epitope prediction of esxA full gene from MDR-TB patientsMaterial and Methods:Total of 24 MDR-TB sputum isolate from TB patients at Dr. Soetomo Hospital were collected from September to December 2016. Samples were confirmed as MDR-TB using GeneXpert and Bactec MGIT 960. Those samples tested using PCR targeted 580 bp of esxA gene and sequencing. Gene sequence was aligned against wild type using Bioedit program version 7.2.5 and NCBI BLAST. T cell epitope prediction was analyzed by GENETYX version 10.Results:Epitope predictions that could be obtained were IEAAAS, ASAIQG, VTSIHS, TKLAAA, VTGMFA based IAd Pattern Position and EAAAS based Rothbard/Taylor Pattern Position. Those prediction epitopes can determine the severity of disease, therefore full gene of esxA could be used as diagnostic target.Conclusion:This research discovered five specific T cell epitope prediction based on IAd Pattern Position and one epitope prediction according to Rothbard/Taylor Pattern Position.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 87%

T Cell Epitopes of the Esxa Full Gene of Mycobacterium Tuberculosis From Sputum of MDR-Tb Patients

Dewi

Soedarsono

Mertaniasih

2018

AJID

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“…This indicated that no mutation had occurred in the esxA gene of MDR-TB from either the moderate and severe group or the mild group. Previous studies analyzing ESAT-6, Ag85B, and Ag85C sequences also showed that in the esxA gene, a singlenucleotide polymorphism was not found (Mertaniasih et al, 2016).…”

Section: Discussionmentioning

confidence: 89%

“…A previous study only attempted to capture 300 bp, 306 bp, 320, 339 bp, and 351 bp of the esxA gene, and the sequence also revealed 100% homology with M. tuberculosis H37Rv. However, most of them were used for TB diagnostic development or vaccine development (Gao et al, 2015;Mertaniasih et al, 2016;Soleimanpour et al, 2016;Dewi et al, 2017a). Therefore, the current study attempted to analyze esxA gene sequences of longer size, intended to capture the full gene and linker genes flanking the esxA gene; thus, the full length of the esxA gene could be analyzed.…”

Section: Discussionmentioning

confidence: 99%

Severity of Tb Classified by Modified Bandim Tb Scoring Associates With the Specific Sequence of Esxa Genes in MDR-Tb Patients

Dewi

Mertaniasih

Soedarsono

2020

AJID

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Background: The severity of pulmonary TB and detection of multidrug-resistant (MDR-TB) TB strains as potential causative agents could be crucial for the determination of treatment success. This study aimed to analyze the association between the specific sequences of the full esxA gene from MDR-TB sputum isolates and the severity class of MDR-TB patients. Material and Methods: A total of 98 sputum samples that were suspected to be MDR-TB were collected from Dr. Soetomo, Surabaya, Indonesia, from September to December 2016. A total of 24 isolates from the 98 patients were confirmed to have positive MDR-TB based on the GeneXpert test. MDR-TB isolates were tested using PCR targeting 580 bp encompassing the full esxA gene, and the resulting amplicon was sequenced. The severity class of the pulmonary TB patients was assessed using modified Bandim TB scoring. Results: The patient severity classification resulted in a moderate and severe degree of TB in 38% and a mild degree of TB in 63% of patients. Visualization of the PCR results showed that all MDR-TB samples were positive for the 580 bp band, and the sequence results showed 100% homology with that of the virulent wild-type M. tuberculosis H37Rv (NC_000962.3). Conclusions: In the current study, an association between the characteristics of the full esxA gene and the severity class of MDR-TB patients is yet to be found. However, the homologous sequence of all samples, associated with various degrees of disease severity, possess 100% identity with that of wild-type M. tuberculosis H37Rv.

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“…Tuberculosis (TB) caused by the bacterial pathogen Mycobacterium tuberculosis is a chronic infection that affects the respiratory system, especially the lungs, and invades various tissues and organs, such as bones and central nervous system. [1][2][3] TB can be passed from an individual to others through droplets when coughing, sneezing, or conversing. Currently, there are 10.4 million new cases of TB over the globe with a total of 1.7 million deaths since 2013 through nowadays.…”

Section: Introductionmentioning

confidence: 99%

Novel Potential Immune Response Biomarkers to Multidrug-Resistant Tuberculosis in the Last Five Years

Unicha

Artama

Nastiti

2022

IJTID

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Rapid and accurate detection performs an important role in the control of raising MDR-TB. Currently, studies on biomarkers as targets for TB diagnostic tests using immune response products to indicate the presence, mycobacterial load, early markers, and activity, diﬀ erentiation, and progression markers of TB infection are rapidly available. This systematic review aims to summarize the last ﬁ ve years of potential biomarkers studies from the immune response for MDR-TB rapid diagnostic development. The authors performed a literature search on four databases as ProQuest, EBSCO Academic Search, Universitas Gadjah Mada Online Library Journal Database, and Google Scholar, retrieved from January 2016 to December 2021. In total, 18,288 articles were identiﬁ ed and three tudies met the inclusion criteria. Several promising biomarkers were found for MDR-TB diagnosis purposes, such as sCD14, PGLYRP2, FGA, Indoleamine 2, 3- dioxygenase (IDO), and Complement Receptor 2 (CR2). A combination of sCD14, PGLYRP2, and FGA were bringing a diagnostic design with a higher sensitivity (94.7%) and speciﬁ city (80%) than the design of a single protein. Higher IDO activity towards the MDR-TB group than in the DS-TB group with a sensitivity of 87.50 %, speciﬁ city of 72.22 %. CR2 was the main focus due to its association with IL-6. After induction of CR2 peptide in a dose-dependent manner, the expression level of IL-6 was decreased signiﬁ cantly. It might because of CR2 peptide regulating the macrophages proinﬂ ammatory cytokines secretion to decrease the local inﬂ ammation of the immune response. These biomarkers are strong candidates for MDR-TB diagnosis due to their important role as the pathogenesis marker of MDR-TB. There is a need of further research to investigate those immune response products and their role to eliminate infection of Mycobacterium tuberculosis directly.

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Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85C Proteins from Clinical Isolates of Mycobacterium tuberculosis

Cited by 7 publications

References 12 publications

T Cell Epitopes of the Esxa Full Gene of Mycobacterium Tuberculosis From Sputum of MDR-Tb Patients

T Cell Epitopes of the Esxa Full Gene of Mycobacterium Tuberculosis From Sputum of MDR-Tb Patients

Severity of Tb Classified by Modified Bandim Tb Scoring Associates With the Specific Sequence of Esxa Genes in MDR-Tb Patients

Novel Potential Immune Response Biomarkers to Multidrug-Resistant Tuberculosis in the Last Five Years

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