Sequence analysis of the gene (6) encoding the major capsid protein (VP6) of group C rotavirus: Higher than expected homology to the corresponding protein from group A virus

Brémont, Michel; Chabanne-Vautherot, Danièle; Vannier, P.; McCrae, Malcolm A.; Cohen, Jean

doi:10.1016/0042-6822(90)90357-w

Cited by 30 publications

(14 citation statements)

References 17 publications

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“…While nucleic and deduced protein sequences have been determined for each of the 11 group A rotavirus genes, very little is known on their non group A counterpart. Recently, several genes coding for structural proteins of group C and group B have been determined [3,4,7,12,14,21]. We have now sequenced a set of non structural gene products of the Cowden strain of group C rotavirus.…”

Section: Introductionmentioning

confidence: 98%

Sequence analysis of three non structural proteins of a porcine group C (Cowden strain) rotavirus

Brémont

Chabanne-Vautherot

Cohen

1993

Archives of Virology

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Sequences of three gene products of a group C (Cowden strain) rotavirus are presented and compared with the sequences of the corresponding group A (SA11) proteins. The degree of similarity for gene 7, 9, and 10 is respectively 34%, 58%, and 45%. Comparison of these 2 viruses allowed to identify several regions well conserved. In the protein coded by Cowden segment 7 (NS 53) only a short cystein and histidine rich region, presenting the zinc finger consensus motif, is common to group A (segment 5) and group C sequences. Conversely the protein coded by segment 9 (NS 35) presented marked homology with group A NS 35. The protein coded by segment 10 (NS 26) is serine rich and presents an accumulation of charged residues near the carboxy terminus, like group A counterpart. This genomic segment presented a single large open reading frame, that contrasts with the group A counterpart for which a second out of phase ORF is used in rotavirus infected MA 104 cells.

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Section: Introductionmentioning

confidence: 98%

Sequence analysis of three non structural proteins of a porcine group C (Cowden strain) rotavirus

Brémont

Chabanne-Vautherot

Cohen

1993

Archives of Virology

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“…The latter is highly conserved in the 11 genes, except for slight differences in genes two (UAUGACC) and three (CGUGACC). The 3' and 5' consensus sequences are different from one rotavirus serogroup to another, for example in group C rotaviruses the 3' end consensus sequence (3'Ccs) is UGUGGCU (Bremont et al, 1990;Qian et al, 1991a,b). Reassortment between rotavirus strains of different serogroups has not been observed (Yolken et al, 1988) and is thought to be restricted by these different consensus sequences.…”

Section: Introductionmentioning

confidence: 99%

Four nucleotides are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3.

1994

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The interaction of the group A rotavirus non‐structural protein NSP3 (NSP3A) with RNA has been studied in vitro. Using semi‐purified NSP3A protein expressed by a recombinant baculovirus and in vitro synthesized RNA, we determined by UV cross‐linking and gel retardation assays that NSP3A binds, in a sequence‐specific manner, the consensus sequence (AUGUGACC) present on the 3′ ends of all group A rotavirus mRNAs. Using short oligoribonucleotides, we established that the minimal RNA sequence required for binding of NSP3A is GACC. Modifications of the UGACC oligonucleotide sequence impaired binding of the protein to the RNA. Furthermore, the recombinant NSP3 protein from rotavirus group C showed specificity for the 3′ end consensus sequence (AUGUGGCU) of only group C mRNAs. Sequence analysis of the NSP3 proteins did not reveal significant homologies with other RNA binding proteins, thus the NSP3 proteins of rotaviruses are the prototypes of a new kind of sequence‐specific RNA binding protein.

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“…Hybridization was performed at 42 °C and the film was exposed 24h. a Wa (GpA); b Cowden (Gp C); c 88-220 (Gp C) the VP 6 of the porcine Cp C Cowden strain has been sequenced and was shown to be 55% homologous with the GpA gene6 [4]. Whether these data taken together indicate functional homology among different rotavirus groups in proteins encoded by these genes remains to be determined.…”

mentioning

confidence: 96%

Comparison of human and porcine group C rotaviruses by Northern blot hybridization analysis

Qian

Saif

Kapikian

et al. 1991

Archives of Virology

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The genetic relationship between human and porcine Gp C rotaviruses and between Gp C and Gp A or B rotaviruses was examined by Northern blot hybridization. Cross-hybridization studies using radiolabeled ssRNA transcript probes demonstrated that the human and porcine Gp C rotaviruses shared a high degree of nucleotide sequence homology in most of the eleven gene segments; the greatest sequence divergence was observed in gene 7. Neither the human nor the porcine Gp C probe hybridized strongly with gene segments from Gp A reference strains or a Gp B bovine rotavirus. These data indicate that genetically, porcine and human Gp C rotaviruses are closely related, whereas they are quite distinct from Gp A or B suggesting that porcine and human Gp C rotaviruses may have evolved from a common ancestral source.

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Sequence analysis of the gene (6) encoding the major capsid protein (VP6) of group C rotavirus: Higher than expected homology to the corresponding protein from group A virus

Cited by 30 publications

References 17 publications

Sequence analysis of three non structural proteins of a porcine group C (Cowden strain) rotavirus

Sequence analysis of three non structural proteins of a porcine group C (Cowden strain) rotavirus

Four nucleotides are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3.

Comparison of human and porcine group C rotaviruses by Northern blot hybridization analysis

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