Sequence and expression of Drosophila Antigen 5-related 2, a new member of the CAP gene family

Megraw, Timothy L.; Kovalick, Gae E.

doi:10.1016/s0378-1119(98)00489-2

Cited by 36 publications

(35 citation statements)

References 26 publications

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“…AG5-related salivary products are members of a group of secreted proteins that belong to the CAP family (cysteine-rich secretory proteins; AG5 proteins of insects; pathogenesis-related protein·1 of plants) (Megraw et al, 1998). The CAP family is related to venom allergens in social wasps and ants (Hoffman, 1993;King and Spangfort, 2000) and to antifungal proteins in plants (Stintzi et al, 1993;Szyperski et al, 1998).…”

Section: Antigen 5 (Ag5) Familymentioning

confidence: 99%

An updated catalogue of salivary gland transcripts in the adult female mosquito,Anopheles gambiae

Arcà

Lombardo

Valenzuela

et al. 2005

Journal of Experimental Biology

172

311

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Salivary glands of blood-sucking arthropods contain a variety of compounds that prevent platelet and clotting functions and modify inflammatory and immunological reactions in the vertebrate host. In mosquitoes, only the adult female takes blood meals, while both sexes take sugar meals. With the recent description of the Anopheles gambiae genome, and with a set of ~3000 expressed sequence tags from a salivary gland cDNA library from adult female mosquitoes, we attempted a comprehensive description of the salivary transcriptome of this most important vector of malaria transmission. In addition to many transcripts associated with housekeeping functions, we found an active transposable element, a set of Wolbachia-like proteins, several transcription factors, including Forkhead, Hairy and doublesex, extracellular matrix components and 71·genes coding for putative secreted proteins. Fourteen of these 71·proteins had matching Edman degradation sequences obtained from SDS-PAGE experiments. Overall, 33·transcripts are reported for the first time as coding for salivary proteins. The tissue and sex specificity of these protein-coding transcripts were analyzed by RT-PCR and microarray experiments for insight into their possible function. Notably, two gene products appeared to be differentially spliced in the adult female salivary glands, whereas 13·contigs matched predicted intronic regions and may include additional alternatively spliced transcripts. Most An. gambiae salivary proteins represent novel protein families of unknown function, potentially coding for pharmacologically or microbiologically active substances. Supplemental data to this work can be found at http:// www.ncbi.nlm.nih.gov/projects/omes/index.html#Ag2.

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Section: Antigen 5 (Ag5) Familymentioning

confidence: 99%

An updated catalogue of salivary gland transcripts in the adult female mosquito,Anopheles gambiae

Arcà

Lombardo

Valenzuela

et al. 2005

Journal of Experimental Biology

172

311

View full text Add to dashboard Cite

show abstract

“…This is a family of secreted proteins that belong to the CAP family (cysteine-rich secretory proteins; AG5 proteins of insects; pathogenesis-related protein 1 of plants) (Megraw et al, 1998). The CAP family is related to venom allergens in social wasps and ants (Hoffman, 1993;King and Spangfort, 2000) and to antifungal proteins in plants (Stintzi et al, 1993;Szyperski et al, 1998).…”

Section: -Antigen-5 Protein Familymentioning

confidence: 99%

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas’ disease

Assumpção

Francischetti

Andersen

et al. 2008

Insect Biochemistry and Molecular Biology

117

132

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Triatoma infestans is a hemiptera, vector of Chagas' disease, that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anti-clotting, anti-platelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its salivary glands was randomly sequenced. Also, salivary proteins were submitted to two dimentional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1,534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results.

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“…This is a family of secreted proteins that belong to the CAP family (cysteine-rich secretory proteins; Ag5 proteins of insects; pathogenesis-related protein 1 of plants) (Megraw et al, 1998). The CAP family is related to venom allergens in social wasps and ants (Hoffman, 1993;King and Spangfort, 2000) and to antifungal proteins in plants (Stintzi et al, 1993;Szyperski et al, 1998).…”

Section: Ag5 Familymentioning

confidence: 99%

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

Calvo

Pham

Ribeiro

2008

Insect Biochemistry and Molecular Biology

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All adult mosquitoes take sugar meals, and most adult females also take blood meals to develop eggs. Salivary glands (SG) of males are thus much smaller and do not contain many of the antihemostatic and antiinflammatory compounds found in females. In the past 5 years, transcriptome analyses have identified nearly 70 different genes expressed in adult female SG. For most of these, no function can be assigned in either blood or sugar feeding. Exceptionally, Toxorhynchites mosquitoes are unusual in that they never feed on blood, and the SG of adults are identical in both sexes. Transcriptome analysis of the adult SG of this mosquito was performed to increase knowledge of the evolution of blood feeding-and to identify polypeptide families associated with sugar feeding-in mosquitoes.

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Sequence and expression of Drosophila Antigen 5-related 2, a new member of the CAP gene family

Cited by 36 publications

References 26 publications

An updated catalogue of salivary gland transcripts in the adult female mosquito,Anopheles gambiae

An updated catalogue of salivary gland transcripts in the adult female mosquito,Anopheles gambiae

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas’ disease

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

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