1993
DOI: 10.1126/science.8380940
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Sequence and Genome Organization of a Human Small Round-Structured (Norwalk-Like) Virus

Abstract: Small round-structured viruses (SRSVs), also known as Norwalk or Norwalk-like viruses, are the major worldwide cause of acute, epidemic nonbacterial gastroenteritis in humans. These viruses, which contain a single-stranded RNA genome, have remained refractory to molecular characterization because of the small amounts of virus in clinical samples and the absence of an animal model and an in vitro culture system. The complete genomic nucleotide sequence of an SRSV, Southampton virus, was determined. The 7696-nuc… Show more

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Cited by 344 publications
(242 citation statements)
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“…In addition, in all published SLV strains except a human strain, London/92 and a porcine strain, PEC/Cowden [5,15,18,27,29,40], an additional ORF has been predicted in +1 frame, overlapping the N terminus of the capsid gene (capsid overlap). To date, the sequence of a ∼3-kb region extending from the RNA polymerase gene to the 3 poly(A) tail is available in GenBank for 9 NLV strains, of which 6 have had their entire genome sequenced: Norwalk/68 [17], Southampton/91 [22], Lordsdale/93 [9], Camberwell/94 [3,36], Chiba/87 (GenBank accession number No. AB042808) and Jena/80 [26].…”
Section: Introductionmentioning
confidence: 99%
“…In addition, in all published SLV strains except a human strain, London/92 and a porcine strain, PEC/Cowden [5,15,18,27,29,40], an additional ORF has been predicted in +1 frame, overlapping the N terminus of the capsid gene (capsid overlap). To date, the sequence of a ∼3-kb region extending from the RNA polymerase gene to the 3 poly(A) tail is available in GenBank for 9 NLV strains, of which 6 have had their entire genome sequenced: Norwalk/68 [17], Southampton/91 [22], Lordsdale/93 [9], Camberwell/94 [3,36], Chiba/87 (GenBank accession number No. AB042808) and Jena/80 [26].…”
Section: Introductionmentioning
confidence: 99%
“…Since the initial cloning and sequencing of the NV genome (4, 10), many other norovirus sequences have been reported (11,12). Despite the availability of cloned genomes, molecular studies of NV replication and development of antiviral drugs have been hampered by the lack of a suitable in vitro culture system and an animal model.…”
mentioning
confidence: 99%
“…Expression of VP1 in insect cells infected with baculovirus recombinants results in the self-assembly of empty recombinant virus-like particles (VLPs) (6, 7). VP2 is also incorporated into VLPs when VP2 is coexpressed with VP1 (8), and its presence increases the yield and stability of VLPs (9).Since the initial cloning and sequencing of the NV genome (4, 10), many other norovirus sequences have been reported (11,12). Despite the availability of cloned genomes, molecular studies of NV replication and development of antiviral drugs have been hampered by the lack of a suitable in vitro culture system and an animal model.…”
mentioning
confidence: 99%
“…NV causes outbreaks of acute non-bacterial gastroenteritis in humans, and the genome of NV is approximately 7.6 kb in size. The genome organization shows three open reading frames (ORFs) encoding non-structural proteins, a capsid protein and a protein whose function is not known (9,15). The capsid protein of NV has an apparent molecular mass of 60 kDa.…”
mentioning
confidence: 99%