Sequence and Organization of the Mamestra configurata Nucleopolyhedrovirus Genome

Li, Qianjun; Donly, Cam; Li, Lulin; Willis, Leslie G.; Theilmann, David A.; Erlandson, Martin A.

doi:10.1006/viro.2001.1313

Cited by 110 publications

(85 citation statements)

References 69 publications

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“…2). This confirms that MacoNPV-A enhancin is expressed as a late gene in the AcMNPV-enMP2 recombinant (Li et al, 2003), and it was translated and incorporated into ODVs as it is in the native MacoNPV-A virus (Fig. 2).…”

Section: Characterization Of Maconpv-a Enhancinsupporting

confidence: 74%

“…These studies revealed that betabaculovirus enhancins are associated with OBs . Studies on alphabaculovirus enhancins are limited and relate mostly to LdMNPV (Bischoff & Slavicek, 1997) and MacoNPV-A (Li et al, 2003). These studies revealed that alphabaculovirus enhancins have a transmembrane domain (Bischoff & Slavicek, 1997) and are present in ODV envelopes in association with nucleocapsids (Slavicek & Popham, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Further evidence for this was provided by experiments that used a recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) expressing TnGV enhancin (Lepore et al, 1996). Similar approaches have been used for alphabaculoviruses; for example, the LD 50 value of a recombinant AcMNPV expressing MacoNPV enhancin was 4.4 times lower than that of wild-type AcMNPV in secondinstar Trichoplusia ni larvae (Li et al, 2003). As well, deletion of enhancin genes in Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) decreased virus potency, confirming that it enhances activity of this virus (Bischoff & Slavicek, 1997;Popham et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…MacoNPV-A enhancin (GenBank accession no. AAM09197; Li et al, 2003Li et al, , 2005 is an 847 aa protein with a zinc-binding domain typical of metalloproteases. It contains a potential transmembrane domain (aa 804-826) followed by a series of basic amino acid residues at its carboxy terminus.…”

Section: Introductionmentioning

confidence: 99%

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Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins

Toprak

Harris

Baldwin

et al. 2012

Journal of General Virology

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To infect per os, baculovirus virions cross the peritrophic matrix (PM) to reach the midgut epithelium. Insect intestinal mucins (IIMs) are PM proteins that protect the PM and aid passage of the food bolus through the gut. Some baculoviruses, including Mamestra configurata nucleopolyhedrovirus (MacoNPV-A), encode metalloproteases, known as enhancins, that facilitate infection by degrading IIMs. We examined the interaction between MacoNPV-A enhancin and M. configurata IIMs both in vivo and in vitro. Per os inoculation of M. configurata larvae with MacoNPV-A occlusion bodies (OBs) resulted in the degradation of McIIM4 within 4 h of OB ingestion, while McIIM2 was unaffected. The PM recovered by 8 h post-inoculation. To investigate whether enhancin was responsible for the degradation of IIM, a recombinant Autographa californica multiple nucleopolyhedrovirus expressing MacoNPV enhancin (AcMNPVenMP2) was constructed. Enhancin was found to be a component of occlusion-derived virions in AcMNPV-enMP2 and MacoNPV-A. In in vitro assays, McIIM4 was degraded after MacoNPV-A and AcMNPV-enMP2 treatments. Degradation of McIIM4 was inhibited by EDTA, a metalloprotease inhibitor, indicating that the degradation was due to enhancin activity. Thus, MacoNPV-A enhancin is able to degrade major structural PM proteins, but exhibits target substrate specificity. INTRODUCTIONThe family Baculoviridae includes arthropod-specific viruses with large, circular, covalently closed, dsDNA genomes of between 80 and 180 kbp. The family includes four genera: Alphabaculovirus [formerly known as Nucleopolyhedrovirus (NPV)], members of which infect lepidopteran hosts; Betabaculovirus [Granulovirus (GV)]; Gammabaculovirus (hymenopteran-specific NPV); and Deltabaculovirus (dipteran-specific NPV) (Jehle et al., 2006). Baculoviruses have two virion phenotypes, budded virus (BV) and occlusionderived virus (ODV). BVs are formed early in the infection cycle when nucleocapsids bud through the host-cell membrane and acquire an envelope. The BVs are responsible for cell-to-cell transmission of the virus within the host insect. Late in the infection cycle, nucleocapsids are enveloped (single or multiple nucleocapsids per virion) by membranes formed within the infected host-cell nucleus and these virions are subsequently occluded within proteinaceous crystals or occlusion bodies (OBs). These ODVs are infectious to insect midgut cells upon ingestion by a susceptible host.In order for baculovirus ODVs to infect insect midgut cells, they must first cross the peritrophic matrix (PM), a semipermeable sheath that is composed of chitin microfibrils and proteins, lining the midgut of most insect species (Wang & Granados, 1997a). The PM protects the midgut epithelium from mechanical damage, toxic compounds and pathogens, regulates nutrient uptake by compartmentalizing digestive processes and prevents excretion of digestive enzymes by providing a means for enzyme recycling (reviewed by Hegedus et al., 2009). The protective feature of the PM is in part due to insect intesti...

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Section: Characterization Of Maconpv-a Enhancinsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins

Toprak

Harris

Baldwin

et al. 2012

Journal of General Virology

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“…AAB47865); LoobMNPV, Lonomia obliqua MNPV (accession no. AAF98122); MacoNPV-A, Mamestra configurata NPV strain 90/2 (Li et al, 2002b); MacoNPV-B, Mamestra configurata NPV strain 96B (Li et al, 2002a); MadiNPV, Malacosoma disstria NPV (accession no. AAD00095); ManeNPV, Malacosoma neustria NPV (accession no.…”

Section: Comparison Of Romnpv and Acmnpv Orfsmentioning

confidence: 99%

Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses

Harrison

Bonning

2003

Journal of General Virology

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The Rachiplusia ou multiple nucleopolyhedrovirus (RoMNPV) is a variant of Autographa californica MNPV (AcMNPV) but is significantly more virulent against several major agricultural pests. The genome sequence of the R1 strain of RoMNPV was determined and compared to that of AcMNPV strain C6. The RoMNPV genome is approximately 131?5 kbp with a G+C content of 39?1 %. The homologous repeat regions (hrs) described for AcMNPV-C6 are present in RoMNPV-R1 but the hrs of RoMNPV have fewer palindromic repeats. The RoMNPV-R1 nucleotide sequence is almost completely collinear with the sequence of AcMNPV-C6 and contains homologues of 150 of the 155 ORFs described for AcMNPV-C6. Deletions, insertions and substitutions have resulted in the loss of homologues for AcMNPV ORFs ac2 (bro), ac3 (ctl), ac97, ac121 and ac140 from the RoMNPV genome. The average amino acid sequence identity between RoMNPV and AcMNPV ORFs is 96?1 % and there are differences in promoter motif composition for 23 of these ORFs. Maximum-likelihood analysis of selection pressures on AcMNPV and RoMNPV ORFs indicate that ORFs ro18/ac20-ac21 (arif-1) and ro135/ac143 (odv-e18) have undergone positive selection.

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Identification and Genomic Analysis of a Second Species of Nucleopolyhedrovirus Isolated from Mamestra configurata

Li¹,

Donly

et al. 2002

Virology

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MacoNPV-96B is a nucleopolyhedrovirus isolated from naturally infected Mamestra configurata (Lepidoptera: Noctuidae) larvae. It was initially identified due to its completely different restriction endonuclease profile relative to the previously sequenced Mamestra configurata virus MacoNPV-90/2 (Q. Li, C. Donly, L. Li, L. G. Willis, D. A. Theilmann, and M. Erlandson, 2002, Virology 294, 106-121). The MacoNPV-96B host range and virulence were also found to differ significantly from those of the previous isolate. To further understand the complex of viruses infecting M. configurata, the genome of MacoNPV-96B was completely sequenced and analyzed in comparison with the genome of MacoNPV-90/2 and other sequenced baculoviruses. MacoNPV-96B consists of 158,482 bp, and 168 open reading frames (ORFs) of 150 nucleotides or longer with minimal overlap have been identified. The genome of MacoNPV-96B is 3422 bp larger than MacoNPV-90/2 and although gene arrangement is virtually identical, there are 9 ORFs unique to MacoNPV-96B and 10 unique to MacoNPV-90/2. bro genes were found to be associated with nonhomologous regions, suggesting that bro genes may facilitate recombination between genomes. A major difference in the gene content between the two viruses is a 5.4-kb insert in MacoNPV-96B, which is highly homologous to a cluster of Xestia c-nigrum granulovirus (XecnGV) ORFs, suggesting recent recombination events between these two viruses. Nucleotide sequence and amino acid sequence identity between the common ORFs of MacoNPV-96B and MacoNPV-90/2 average 87 and 90%, respectively. The sequence data suggest that MacoNPV-96B and MacoNPV-90/2 are closely related but have diverged and evolved into two separate species. This is the first study to identify highly related but separately evolving viruses in the same insect host and geographic location. A new Identity-GeneParityPlot analysis was developed to perform a comparison of two viral genomes in gene content and arrangement as well as homology level of individual ORFs.

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Sequence and Organization of the Mamestra configurata Nucleopolyhedrovirus Genome

Cited by 110 publications

References 69 publications

Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins

Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins

Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses

Identification and Genomic Analysis of a Second Species of Nucleopolyhedrovirus Isolated from Mamestra configurata

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