Sequence and phylogenetic analysis of the thrombospondin-related adhesive protein gene of Babesia gibsoni isolates in dogs in South India

Bora, Christophe Angeline Felicia; Varghese, Anju; Deepa, Chundayil Kalarickal; Nandini, Ashwathappa; Malangmei, Lanchalung; Kumar, Karapparambu Gopalan Ajith; Raina, O.K.; John, Lijo; Prasanna, Preena; Asaf, Muhasin; Kumar, Gatchanda Shravan; Hembram, Prabodh Kumar; Vergis, Jess; Juliet, Sanis; Ravindran, Reghu

doi:10.1016/j.parint.2021.102477

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…More importantly, Babesia is easily confused with early-stage Plasmodium [3,6,9,10] and can lead to unsuccessful treatment with antimalarial drugs. PCR-based molecular diagnostic approaches are important supplementary methods for accurate diagnosis [11][12][13][14][15], but these methods are usually not feasible in resource-limited areas due to equipment requirements and cost.…”

Section: Introductionmentioning

confidence: 99%

The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper

Tao

Zhang

et al. 2022

Journal of Tropical Medicine

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Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.

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