Sequence‐based characterization of the eight SLA loci in Korean native pigs

Lee, Y. J.; Cho, K. H.; Kim, M. J.; Smith, Douglas M.; Ho, Chak-Sum; Jung, Kyu-In; Jin, Dong Il; Park, C. S.; Jeon, J. T.; Lee, J. H.

doi:10.1111/j.1744-313x.2008.00775.x

Cited by 20 publications

(16 citation statements)

References 8 publications

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“…DRB1*1001, DQB1*0601, DQA*0101 and DRA*020102 were associated with haplotype Hp-0.23. This result is consistent with previously findings in Korean native pig population (24,36). Haplotype Hp-0.13 has been reported in commercial porcine lines and also in Pietrain populations (25,35), which matches our results for DRB1, DQB1 and DQA loci ( Table 3).…”

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confidence: 93%

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Characterization of swine leucocyte antigen alleles in a crossbred pig to be used in xenotransplant studies

et al. 2014

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We have characterized swine leucocyte antigen (SLA) classes I and II molecules of a domestic pig as a model for use in our xenotransplant program. Molecular characterization of the SLA classes I and II genes is critical to understanding the adaptive immune responses between swine and humans in the event of xenotransplantation. Seven swine leucocyte antigen genes (SLA-1, SLA-2, SLA-3, DQB1, DRB1, DQA and DRA) were analyzed and 15 alleles were identified. A novel DRA*w04re01 is reported for this limited polymorphic class II gene. The heterozygous haplotypes, Hp-32.0/35.0 and Hp-0.13/0.23 were deduced for our IU-pig model, for SLA classes I and II regions, respectively.

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confidence: 93%

“…With respect to the SLA class II loci, two alleles per locus were detected. Seven alleles have been reported previously in several populations, such as the Austrian purebred Pietrain, Hanford swine, Korean native pigs as well as in outbred pig populations (34)(35)(36).…”

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confidence: 95%

Characterization of swine leucocyte antigen alleles in a crossbred pig to be used in xenotransplant studies

et al. 2014

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“…Therefore, the four confirmed allelic sequences were designated as: SLA-1 *07:04 (KU754554), SLA-1 *08:11 (KU754558), SLA-2 *16:03 (KU754568), and SLA-2 *10:06 (KU754570), respectively, by the Committee. Furthermore, SLA-1 *08pt13, SLA-1 *08sk11, SLA-1 *11jh02, SLA-2 *w09pt22, SLA-2 *10es21, SLA-2 *10sk21, SLA-3 *03pt31, and SLA-3 *hb06 alleles had previously been identified in other pig breeds and some cell lines, e.g., Korean native pigs, Danish swine, Landrace pigs, cell lines “ESK-4, PT-K75, SK-RST,” and so on (18–21). These alleles were therefore assigned the following permanent names by the SLA Nomenclature Committee: SLA-1 *08:07 (KU953375), SLA-1 *08:08 (KU754548), SLA-1 *11:03 (KU754550), SLA-2 *09:03 (KU953376), SLA-2 *10:04 (KU754565), SLA-2 *10:05 (KU754566), SLA-3 *03:06 (KU953377), and SLA-3 *04:04 (KU754583), respectively.…”

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confidence: 99%

Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs

et al. 2017

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The highly polymorphic swine major histocompatibility complex (MHC), termed swine leukocyte antigen (SLA), is associated with different levels of immunologic responses to infectious diseases, vaccines, and transplantation. Pig breeds with known SLA haplotypes are important genetic resources for biomedical research. Canadian Yorkshire and Landrace pigs represent the current specific pathogen-free (SPF) breeding stock maintained in the isolation environment at the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. In this study, we identified 61 alleles at five polymorphic SLA loci (SLA-1, SLA-2, SLA-3, DRB1, and DQB1) representing 17 class I haplotypes and 11 class II haplotypes using reverse transcription-polymerase chain reaction (RT-PCR) sequence-based typing and PCR-sequence specific primers methods in 367 Canadian SPF Yorkshire and Landrace pigs. The official designation of the alleles has been assigned by the SLA Nomenclature Committee of the International Society for Animal Genetics and released in updated Immuno Polymorphism Database-MHC SLA sequence database [Release 2.0.0.3 (2016-11-03)]. The submissions confirmed some unassigned alleles and standardized nomenclatures of many previously unconfirmed alleles in the GenBank database. Three class I haplotypes, Hp-37.0, 63.0, and 73.0, appeared to be novel and have not previously been reported in other pig populations. One crossover within the class I region and two between class I and class II regions were observed, resulting in three new recombinant haplotypes. The presence of the duplicated SLA-1 locus was confirmed in three class I haplotypes Hp-28.0, Hp-35.0, and Hp-63.0. Furthermore, we also analyzed the functional diversities of 19 identified frequent SLA class I molecules in this study and confirmed the existence of four supertypes using the MHCcluster method. These results will be useful for studying the adaptive immune response and immunological phenotypic differences in pigs, screening potential T-cell epitopes, and further developing the more effective vaccines.

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“…Interferon (IFN)‐γ (R & D Systems, Minneapolis, MN, USA) was used at a final concentration of 100 ng/mL in the PK13, PK15 and PT‐K75 cell cultures for 5 days to induce SLA class II mRNA expression. Alleles of SLA‐1, SLA‐3, SLA‐2, SLA‐DRB1 and SLA‐DQB1 were amplified from complementary DNA (cDNA) using the locus‐specific polymerase chain reaction (PCR) primers as previously described (Lee et al ., 2005; Smith et al ., 2005c; Ho et al ., 2006; Lee et al ., 2008). These primers were positioned in the untranslated regions to amplify the entire coding region.…”

Section: Swine Leucocyte Antigen (Sla) Alleles Identified In Eight Comentioning

confidence: 99%

Sequence‐based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines

Franzo-Romain

Lee

et al. 2009

Int J Immunogenetics

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A total of 53 alleles at five highly polymorphic swine leucocyte antigen (SLA) loci (SLA-1, SLA-3, SLA-2, SLA-DRB1, and SLA-DQB1) were identified in eight commercially available porcine cell lines (ESK-4, LLC-PK1, MPK, PK13, PK15, PT-K75, SK-RST, and ST). This information is essential for the use of these cell lines to understand the role of SLA genes and proteins in swine models of transplantation, xenotransplantation, and in swine immune responses to infectious diseases and vaccines. The ready availability of these cell lines also makes them a good source of reference DNA for SLA allele typing.

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Sequence‐based characterization of the eight SLA loci in Korean native pigs

Cited by 20 publications

References 8 publications

Characterization of swine leucocyte antigen alleles in a crossbred pig to be used in xenotransplant studies

Characterization of swine leucocyte antigen alleles in a crossbred pig to be used in xenotransplant studies

Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs

Sequence‐based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines

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