Sequence-Based Typing for Platelet alloantigens

Abstract

Human platelet antigen (HPA) typing is largely performed by use of DNA-based techniques in patients that require assessing the risk of HPA alloimmunization. In this chapter, HPA typing by sequencing-based typing (SBT) techniques is described.

Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing

“…In the present study, we updated the PCR‐SBT method for HPA‐1 to 28bw systems by multiplex PCR. Compared with the previous PCR‐SBT method , our updated method could reduce the labour intensity and technical complexity. Firstly, only six wells were needed to amplify with multiplex primer pairs for genotyping of HPA‐1 to 28bw systems in each sample.…”

“…prevention and treatment of patients with immune platelet disorders and for the provision of compatible blood products from HPA-typed donor panel populations. Currently, DNA-based methods have been widely used for HPA genotyping [8][9][10][11][12][13], such as PCR sequence-specific primers (PCR-SSP) [8], PCR sequence-based typing (PCR-SBT) [9], BeadChip Microarray Technology [10], real-time PCR [11], matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [12,13]. We have also reported a PCR-SBT assay combining with high-throughput DNA sequencer for identifying the genotypes of HPA-1 to 17w systems [14].…”