Sequence comparison of the right end of fowl adenovirus genomes

Abstract

The nucleotide sequence at the right end of the genomes of fowl adenoviruses (FAdVs) representing species groups C (FAdV-4 CA and FAdV-10 C-2B), D (FAdV-2 CA), and E (FAdV-8 CA) was determined and analyzed and compared to FAdV-1 (species group A virus) and FAdV-9 A-2A (species group B virus). High nucleotide sequence identities and amino acid identities (74-94%) were found among viruses in the same species group. Homologues to known open reading frames (ORFs) of the right end of the reported FAdV genomes, such as lipase and Gam-1, were also found in all analyzed viral genomes. Homologues to a few FAdV ORFs were found in viruses other than FAdVs as well. Several novel FAdV ORFs were also found. Most identified ORFs have unknown function and thus further studies are necessary to establish their importance in virus replication. ORF content within the right end of the FAdV genomes varied among FAdVs, while the gene order and orientation of shared ORFs are conserved between different FAdVs.

Whole-genome sequences of two turkey adenovirus types reveal the existence of two unknown lineages that merit the establishment of novel species within the genus Aviadenovirus

“…Following the fiber gene(s), both genomes possessed the same genes as found in FAdV-1 (and with the exception of an additional ORF43 in FAdV-4, also in all the FAdVs sequenced so far): ORF22, -20A, -20, -19, -8 and -17 (Corredor et al, 2008, Kaján et al, 2010Marek et al, 2013). The predicted splicing donor and acceptor sites of the TAdV-5 ORF19 cannot be taken for granted as they were selected arbitrarily from several possibilities identified by bioinformatics (because of the lack of any identifiable homology around the splicing sites).…”

Occurrence of Adenovirus Field Strains in Birds Infected with Marek’S Disease Virus

“…The L1 fragment of the hexon gene is a domain of the highest variability and is responsible for determination of the antigenic properties of the virus (2,4,25). Therefore, this region is very useful for the studies on the phylogenetic typing and genotyping of the adenoviruse, and it was a target for the primers we designed.…”

“…Fowl adenoviruses belonging to subgroup I, subgroups II and III are composed of adenoviruses of turkeys, geese, and falcons. The subgroup I was classified into five species, designated A-E, including 12 serotypes, on the basis of antigenic characteristics and restriction profiles of BamHI and HindIII enzymes (2,13,20).…”

“…Avian adenoviruses, similarly to other adenoviruses, are icosahedral, non-enveloped, dsDNA viruses with capsid 74-80 nm in diameter, constructed of 252 capsomers surrounding a core (2,8). The main protein of the adenovirus capsid is hexon 93-950 aa long and with mass of 103 kDa, forming 240 trimers.…”

A region at the left end of the fowl adenovirus 9 genome that is non-essential in vitro has consequences in vivo

“…FAdV serotypes are grouped into five species (Fowl adenovirus A-E), all belonging to the genus Aviadenovirus, family Adenoviridae (Benkő et al, 2005). Complete nucleotide sequences are available for the genomes of FAdV-1 (CELO virus) and FAdV-9 (strain A-2A) (Chiocca et al, 1996;Ojkic & Nagy, 2000), and partial nucleotide sequences are also available for the left and right ends of the genomes of FAdV-2, -4, -8 and -10 (strain C-2B) (Corredor et al, 2006(Corredor et al, , 2008. None of the early genes at the left and right ends of the FAdV genomes are homologues of the E1, E3 and E4 genes of mastadenoviruses, which infect mammals Chiocca et al, 1996).…”