Sequence conservation of the major outer capsid glycoprotein of human group C rotaviruses

Grice, A S; Lambden, Paul R.; Caul, E.O.; Clarke, Ian N.

doi:10.1002/jmv.1890440209

Cited by 28 publications

(19 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This indicates that the evolution or drift of these genes is slow. It is also very much in keeping with the results of other studies in which the sequences of these human group C rotavirus genes from isolates from a number of different countries were compared and a very high degree of sequence conservation was found (1,3,4,6,9). Our results, including the VP7 and VP4 gene sequence comparisons between the central Australian and overseas isolates, therefore confirm and extend the findings presented in the other studies.…”

Section: Discussionsupporting

confidence: 91%

“…Our results, including the VP7 and VP4 gene sequence comparisons between the central Australian and overseas isolates, therefore confirm and extend the findings presented in the other studies. These results also support the contention that human group C rotaviruses evolved relatively recently and possibly belong to a single globally distributed genotype (3,6,9).…”

Section: Discussionsupporting

confidence: 84%

“…Changes in the sequences of both the VP7 and VP4 genes were evident over the 7-year period but they were few, particularly relative to the differences in sequence between the equivalent human and animal group C rotavirus genes (1,3,4,6,9). This indicates that the evolution or drift of these genes is slow.…”

Section: Discussionmentioning

confidence: 96%

“…Sequence variation is also evident between VP7 genes of human and animal group C rotaviruses, but among human group C rotavirus isolates themselves from many different parts of the world remarkable sequence conservation has been noted in this gene (1,3,6,9,11). A high degree of conservation has also been evident between the sequences of the VP4 genes of human group C rotavirus isolates from different parts of the world, but a greater difference was noted between these sequences and those of the VP4 genes of bovine and porcine group C rotavirus strains (1,4).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Incidence of Group C Human Rotavirus in Central Australia and Sequence Variation of the VP7 and VP4 Genes

et al. 2004

View full text Add to dashboard Cite

Human group C rotavirus was identified in central Australia in each of eight years over a 16-year period between 1982 and 1997. Cases occurred either sporadically but over a relatively short period of time or as clustered outbreaks. These are the only reports of human group C rotavirus in Australia other than that of a single case reported approximately 1,800 km away in 1982. The electrophoretic genome profiles of isolates were identical for all those identified within the same year but different between those identified in different years. The VP7 genes of four isolates identified in four different years over a 7-year period between 1987 and 1993, and the VP4 genes of two of these isolates showed relatively little variation in genome and deduced amino acid sequence upon comparison of the equivalent genes between isolates. The sequences were also very similar to those from the corresponding genes from most of the human group C rotavirus isolates from other countries. This continues the observation of a high degree of gene sequence conservation among human group C rotaviruses worldwide.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

See 2 more Smart Citations

Incidence of Group C Human Rotavirus in Central Australia and Sequence Variation of the VP7 and VP4 Genes

et al. 2004

View full text Add to dashboard Cite

show abstract

“…As infecções pelos rotavirus do grupo C são clinicamente mais severas que as do grupo A, até então relatadas (GRICE et al, 1994).…”

Section: Nomenclaturaunclassified

Caracterização Sorológica E Molecular De Rotavírus De Suínos Na Região Sul Do Brasil

Kroeff¹

2000

RSA

View full text Add to dashboard Cite

O objetivo do estudo foi realizar a caracterização sorológica e molecular em amostras de rotavírus suíno na região Sul do Brasil. Para sua realização, foram colhidas 167 amostras de fezes de suínos nas fases de maternidade e creche em 52 granjas de produção de suínos que apresentavam freqüentes casos de diarréia, em 14 municípios dos Estados do Rio Grande do Sul, Santa Catarina e Paraná, durante o período compreendido entre junho de 1995 e outubro de 1997. Todas as amostras foram submetidas a um ensaio imunoenzimático (ELISA) para a detecção de rotavírus do grupo A, das quais 59 (35,33%) foram positivas. Por meio da eletroforese em gel de poliacrilamida (PAGE) foi possível revelar a existência de um único eletroferótipo de rotavírus suíno nas regiões estudadas. As amostras positivas foram testadas para a caracterização de subgrupo por meio de um ELISA com anticorpos monoclonais (mAb-ELISA), onde 9 amostras foram específicas para o subgrupo ll e 50 não reagiram para nenhum dos subgrupos, sendo classificadas como nãoI-nãoII. Para a caracterização de genótipos G e P utilizou-se a técnica de transcrição reversa, seguida da reação em cadeia da polimerase (RT-PCR) com primers específicos para rotavírus de suínos, bovinos e humanos. As amostras foram caracterizadas como genótipos G3, G4, G5, G9, G10 e P[6], P[7] e misturas de P[6] + P[7] no mesmo animal. Este estudo evidenciou a existência de diferentes genótipos de rotavírus suínos, circulando ao mesmo tempo em uma mesma região e ou em uma mesma granja, incluindo o genótipo G9 que até o último ano tinha sido encontrado somente em amostras humanas.

show abstract

Seroepidemiology of human group C rotavirus in the UK

James

Lambden

Caul³

et al. 1997

J. Med. Virol.

View full text Add to dashboard Cite

The gene coding for the major inner capsid protein VP6 of human group C rotavirus was cloned into baculovirus using the pBlueBac2 vector and expressed in insect cells. When cultured in High Five cells, VP6 was expressed at a high level and exported to the cell culture medium. Purified VP6 was used to immunise rabbits. Hyperimmune rabbit serum, which reacted with native human group C rotavirus in infected cells, was used to develop and optimise an EIA for the detection of antibodies to group C rotavirus using the recombinant VP6 as a source of antigen. In a local epidemiological survey of 1000 sera grouped by age, an average of 43% of samples were found to have antibodies to human group C rotavirus with the highest proportion (66%) in the 71-75 year age group. In comparison, 97% of adults and 85% of children had antibodies to recombinant VP6 from the bovine RF strain of group A rotavirus. These results suggest that infection with human group C rotavirus is a common occurrence despite the apparent rarity of reports of human group C rotavirus in clinical samples from patients with gastroenteritis.

show abstract

Sequence conservation of the major outer capsid glycoprotein of human group C rotaviruses

Cited by 28 publications

References 25 publications

Incidence of Group C Human Rotavirus in Central Australia and Sequence Variation of the VP7 and VP4 Genes

Incidence of Group C Human Rotavirus in Central Australia and Sequence Variation of the VP7 and VP4 Genes

Caracterização Sorológica E Molecular De Rotavírus De Suínos Na Região Sul Do Brasil

Seroepidemiology of human group C rotavirus in the UK

Contact Info

Product

Resources

About