Sequence data of six unusual alleles at SE33 and D1S1656 STR Loci

Alsafiah, Hussain M; Iyengar, Arati; Hadi, Sibte; Alshlash, Waleed M.; Goodwin, William

doi:10.1002/elps.201800191

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…5a). Although this allele could, in principle, belong to D18S1364 forming triplet allele genotype, it was confirmed by sequencing that it belongs to D1S1656 35 . It is not necessary for an allelic ladder to represent all rare alleles, however, alleles outside the designated window of a locus may be misinterpreted especially when adjacent loci are homozygous.…”

Section: Resultsmentioning

confidence: 96%

An evaluation of the SureID 23comp Human Identification Kit for kinship testing

Alsafiah

Aljanabi

Hadi

et al. 2019

Sci Rep

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Short tandem repeat (STR) profiling has been routinely used in kinship testing since the introduction of commercial kits in the mid-1990s. While 15 to 23 STR loci normally give definitive results in simple kinship testing, additional loci are sometimes required to resolve complex cases. The SureID 23comp Human Identification Kit, recently released by Health Gene Technologies (China), multiplexes amelogenin and 22 autosomal STRs, 17 of which are non-CODIS STRs. This enables the profiling of 38–40 loci when used in conjunction with widely used commercial kits. In this study, the kit was evaluated for kinship applications as a supplementary STR kit following the minimum criteria for validation recommended by the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM) using 500 samples. Performance was comparable with other commercial kits demonstrating: repeatability and reproducibility; precision (maximum s.d. 0.1048 nt); accuracy, all alleles were within ±0.41 nt compared to the actual sizes; heterozygous peak balances at all loci >68%; stutter ratios ranged from 3.8% to 16.15%; full profiles were generated with 125 pg DNA (95.12% of alleles at 62 pg),; and we found 100% concordance over 5 common STRs with the GlobalFiler kit.

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Section: Resultsmentioning

confidence: 96%

An evaluation of the SureID 23comp Human Identification Kit for kinship testing

Alsafiah

Aljanabi

Hadi

et al. 2019

Sci Rep

Self Cite

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“…For the concordance study, the sequenced-based data was compared to CE data and Sanger sequencing was used to confirm a discordant result as previously described in [5]. The novelty of a motif pattern or of an allele sequence was assessed based on those motifs and sequences reported in [2] and in STRBase [6].…”

Section: Methodsmentioning

confidence: 99%

Sequence‐based Saudi population data for the SE33 locus

Alsafiah

Khubrani

Hadi

et al. 2019

Forensic Science International: Genetics Supplement Series

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