Sequence diversity in the amino-terminal region of the malaria-vaccine candidate serine repeat antigen in natural Plasmodium falciparum populations

Safitri, Indri; Jalloh, Amadu; Tantular, Indah Setyawati; Pusarawati, Suhintam; Win, Thin Thida; Liu, Qing; Ferreira, Marcelo U.; Dachlan, Yoes Prijatna; Horii, Toshihiro; Kawamoto, Fumihiko

doi:10.1016/s1383-5769(02)00088-0

Cited by 12 publications

(11 citation statements)

References 36 publications

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“…The dimorphic allelic families are not necessarily homogeneous (i.e., there is some within-family heterogeneity); members of different families, however, differ at more than 30% of the amino acid residues in variable sequences (i.e., there is extensive between-family divergence). Allelic dimorphism was originally described for the msp-1 locus (111) and was later found in other blood-stage antigens of P. falciparum, such as the merozoite surface proteins MSP-2 and MSP-3, the serine repeat antigen (SERA), and EBA-175, a parasite molecule that binds to sialic acid and glycophorin A during RBC invasion (22,54,99). The msp-1 locus of P. vivax is also essentially dimorphic (48,86,87).…”

Section: Repetitive Antigens and Allelic Polymorphismmentioning

confidence: 97%

Antigenic Diversity and Immune Evasion by Malaria Parasites

Ferreira

Nunes

Wunderlich

2004

Clin Vaccine Immunol

111

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Section: Repetitive Antigens and Allelic Polymorphismmentioning

confidence: 97%

Antigenic Diversity and Immune Evasion by Malaria Parasites

Ferreira

Nunes

Wunderlich

2004

Clin Vaccine Immunol

111

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“…The gene encoding SERA5 contains four exons, with most sequence diversity observed in exon II (89,90). Most of the diversity in exon II is in the form of indels, and amino acid substitutions were also observed at 17 positions (1·7 kb evaluated) (89).…”

Section: Genetic Diversity In the Leading Vaccine Antigensmentioning

confidence: 99%

Genetic diversity and malaria vaccine design, testing and efficacy: preventing and overcoming ‘vaccine resistant malaria’

Takala

Plowe

2009

Parasite Immunology

192

171

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SUMMARYThe development of effective malaria vaccines may be hindered by extensive genetic diversity in the surface proteins being employed as vaccine antigens. Understanding of the extent and dynamics of genetic diversity in vaccine antigens is needed to guide rational vaccine design and to interpret the results of vaccine efficacy trials conducted in malaria endemic areas. Molecular epidemiological, population genetic, and structural approaches are being employed to try to identify immunologically relevant polymorphism in vaccine antigens. The results of these studies will inform choices of which alleles to include in multivalent or chimeric vaccines; however, additional molecular and immuno-epidemiological studies in a variety of geographic locations will be necessary for these approaches to succeed. Alternative means of overcoming antigenic diversity are also being explored, including boosting responses to critical conserved regions of current vaccine antigens, identifying new, more conserved and less immunodominant antigens, and developing whole-organism vaccines. Continued creative application and integration of tools from multiple disciplines, including epidemiology, immunology, molecular biology, and evolutionary genetics and genomics, will likely be required to develop broadly protective vaccines against Plasmodium and other antigenically complex pathogens.

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“…Most polymorphism has been described in exon II which corresponds to the amino terminal region (47 kDa peptide) of the protein that encodes two repeat domains: the octamer repeats (Nt47 = OR domain) and the serine repeats (SR domain) characterized by a stretch of serine residues. The P126 polymorphisms basically comprise the events of deletions/insertions in these two repetitive domains of the protein, but nucleotide replacements, synonymous or not, were also described [ 21 - 24 ]. The OR region predominately consists of six octamer units (OR-II), but variation in the sequence and number of repeat units (5 octamer units – OR-I) may be seen in different alleles [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon

Pratt-Riccio

Sallenave-Sales

Oliveira-Ferreira

et al. 2008

Malar J

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Background: The Plasmodium falciparum P126 protein is an asexual blood-stage malaria vaccine candidate antigen. Antibodies against P126 are able to inhibit parasite growth in vitro, and a major parasite-inhibitory epitope has been recently mapped to its 47 kDa N-terminal extremity (octamer repeat domain -OR domain). The OR domain basically consists of six octamer units, but variation in the sequence and number of repeat units may appear in different alleles. The aim of the present study was to investigate the polymorphism of P126 N-terminal region OR domain in P. falciparum isolates from two Brazilian malaria endemic areas and its impact on anti-OR naturally acquired antibodies.

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Sequence diversity in the amino-terminal region of the malaria-vaccine candidate serine repeat antigen in natural Plasmodium falciparum populations

Cited by 12 publications

References 36 publications

Antigenic Diversity and Immune Evasion by Malaria Parasites

Antigenic Diversity and Immune Evasion by Malaria Parasites

Genetic diversity and malaria vaccine design, testing and efficacy: preventing and overcoming ‘vaccine resistant malaria’

Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon

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