Sequence Diversity of the Internal Transcribed Spacer (ITS) Region of the rRNA Operons Among Different Serogroups of Legionella pneumophila Isolates

Pérez-Luz, Sara; Fernández, J.; Rodrı́guez-Valera, Francisco; Pascual, Leonor; Moreno, Carmen; Amo, A. Del; Apráiz, D.; Catalán, Vicente

doi:10.1078/0723-2020-00115

Cited by 5 publications

(8 citation statements)

References 28 publications

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“…1998). In previous works (Pérez‐Luz et al. 2002) studying the 16S–23S spacer region in different species of Legionella genus by using the same methodology described in this report, showed that the different species studied displayed a different profile, and in the case of Leg.…”

Section: Discussionsupporting

confidence: 52%

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Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region

Pérez-Luz¹,

Yáñez²,

Catalán³

2004

J Appl Microbiol

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Aim: In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water. Methods and Results: The analysis of the fragments was carried out using a High Resolution acrylamide/ bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population. Conclusion: Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates. Significance and impact of the study: A possible new methodology for a rapid identification of pathogens in water.

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Section: Discussionsupporting

confidence: 52%

“…The same thing occurred with Leg. pneumopila , in which three operons had been described and presented a constant profile consisting in two fragments of 496 and 510 pb (Pérez‐Luz et al. 2002).…”

Section: Discussionmentioning

confidence: 99%

Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region

Pérez-Luz¹,

Yáñez²,

Catalán³

2004

J Appl Microbiol

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“…The known number of tRNA genes occurring in bacterial rrn operons varies from zero to five (4,5,12,17,27,44,46,48,50,51 (17,48). The sequence data analyzed from the 55 ISRs within these 5 C. difficile strains revealed they had a mosaic-like structure, as was also reported for the ISRs in Salmonella enterica, Haemophilus parainfluenzae, Staphylococcus aureus, Vibrio cholerae, Vibrio mimicus, Vibrio parahaemolyticus, Myxobacterium spp., and Acinetobacter baylyi (4,5,12,17,25,27,44,46,48,50,51). The highly mosaic nature of the C. difficile ISR is entirely consistent with the recent finding that the complete genome of C. difficile is also mosaic in nature, with 11% of the genome comprised of mobile genetic elements that in many cases contain repetitive sequences (59).…”

Section: Discussionmentioning

confidence: 79%

“…These sequence data suggest that intraspecific diversity in the ISRs in C. difficile is much greater than that reported for any other bacterial species (4,5,12,15,17,20,27,30,40,50,51). Many authors have compared ISRs between different species and strains of a single bacterial species (15,17,33,44,46).…”

Section: Discussionmentioning

confidence: 93%

The Mosaic Nature of Intergenic 16S-23S rRNA Spacer Regions Suggests rRNA Operon Copy Number Variation in Clostridium difficile Strains

Sadeghifard

Gürtler

Beer

et al. 2006

Appl Environ Microbiol

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Clostridium difficile is a major spore-forming environmental pathogen that causes serious health problems in patients undergoing antibiotic therapy. Consequently, reliable and sensitive methods for typing individual strains are required for epidemiological and environmental studies. Ribotyping is generally considered the best method, but it fails to account for sequence diversity which might exist in intergenic 16S-23S rRNA spacer regions (ISRs) within and among strains of this organism. Therefore, this study was undertaken to compare the sequence of each individual ISR in five strains of C. difficile to explore the extent of this diversity and see whether such information might provide the basis for more sensitive and discriminatory strain typing methods. After targeted PCR amplification, cloning, and sequencing, the diversity of the ISRs was used as a measure of rRNA operon copy number. In C. difficile strains 630, ATCC 43593, A, and B, 11, 11, 7, and 8 ISR length variants, respectively, were found (containing different combinations of sequence groups [i to xiii]), suggesting 11, 11, 7, and 8 rrn copies in the respective strains. Many ISRs of the same length differed markedly in their sequences, and some of these were restricted in occurrence to a single strain. Most of these ISRs did not contain any tRNA genes, and only single copies of the tRNA Ala gene were found in those that did. The presence of ISR sequence groups (i to xiii) varied between strains, with some found in one, two, three, four, or all five strains. We conclude that the intergenic 16S-23S rRNA spacer regions showed a high degree of diversity, not only among the rrn operons in different strains and different rrn copies in a single strain but also among ISRs of the same length. It appears that C. difficile ISRs vary more at the inter-and intragenic levels than those of other species as determined by empirical comparison of sequences. The precise characterization of these sequences has demonstrated a high level of mosaic sequence block rearrangements that are present or absent in multiple strain-variable rrn copies within and between five different strains of C. difficile.

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“…However, intragenomic variability (mosaic-like structure) has been also detected in the bacterial ITS [2][3][4][5][6]. To date, mosaic-like structures have been detected in the non-thermophilic bacteria Salmonella enterica, Haemophilus parainfluenzae, Staphylococcus aureus, Vibrio cholerae, Vibrio mimicus, Vibrio parahaemolyticus, Myxobacterium spp., Acinetobacter baylyi, Photobacterium damselae and Clostridium difficile [2,3,5,[7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Unusual intragenomic and interspecific variability of Geobacillus 16S-23S rRNA internal transcribed spacers

2008

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Sequence Diversity of the Internal Transcribed Spacer (ITS) Region of the rRNA Operons Among Different Serogroups of Legionella pneumophila Isolates

Cited by 5 publications

References 28 publications

Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region

Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region

The Mosaic Nature of Intergenic 16S-23S rRNA Spacer Regions Suggests rRNA Operon Copy Number Variation in Clostridium difficile Strains

Unusual intragenomic and interspecific variability of Geobacillus 16S-23S rRNA internal transcribed spacers

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