Sequence, genomic organization of the EcoRI-A fragment of Autographa californica nuclear polyhedrosis virus, and identification of a viral-encoded protein resembling the outer capsid protein VP8 of rotavirus

Braunagel, Sharon C.; Daniel, K; Reilly, Linda M.; Guarino, Linda A.; Hong, Tao; Summers, Max D.

doi:10.1016/0042-6822(92)90281-s

Cited by 15 publications

(5 citation statements)

References 18 publications

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“…AcMNPV carries two IAP homologues, AcIAP1 and AcIAP2, which apparently are not sufficient to block viralinduced apoptosis in SF-21 cells as P35 mutant viruses rapidly undergo apoptosis upon infection of SF-21 cells (Ayres et al, 1994;Braunagel et al, 1992;Clem et al, 1991;Clem and Miller, 1993). OpMNPV carries four IAP homologues (Ahrens et al, 1997;Birnbaum et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Anti- and pro-apoptotic activities of baculovirus and Drosophila IAPs in an insect cell line

Harvey

Kaiser

et al. 1997

Cell Death Differ

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The anti-apoptotic activities of two baculovirus IAPs, OpIAP and CpIAP, were directly compared with that of two Drosophila IAPs, DIAP1 and DIAP2, in the same insect cell line, SF-21 cells. Like OpIAP and CpIAP, DIAP1 inhibited actinomycin Dinduced apoptosis and apoptosis induced by Doom. Removal of the RING finger of DIAP1 reduced but did not eliminate its anti-apoptotic activity. DIAP2 was unable to inhibit actinomycin-D induced apoptosis but was able to partially inhibit Doominduced apoptosis. The baculoviral BIR and RING finger regions, when separated, were unable to block apoptosis induced by actinomycin D or Doom. Instead, the BIR regions of OpIAP and CpIAP as well as the RING finger regions of CpIAP and DIAP1 induced apoptosis. Thus, there were significant differences in the manner in which the different domains of the viral and cellular homologues of IAPs interacted with the components of the pathways regulating apoptosis in SF-21 cells.

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Section: Discussionmentioning

confidence: 99%

Anti- and pro-apoptotic activities of baculovirus and Drosophila IAPs in an insect cell line

Harvey

Kaiser

et al. 1997

Cell Death Differ

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“…la) was cloned into pBluescript KS(+) (Stratagene). Sequencing reactions were performed as previously described (Braunagel et al, 1992). Computer analysis of the pk-1 gene and its predicted protein product were conducted using the programs of Genetics Computer Group (version 7, 1991 ;Devereux et al, 1984).…”

Section: Methodsmentioning

confidence: 99%

The pk-1 Gene of Autographa Californica Multinucleocapsid Nuclear Polyhedrosis Virus Encodes a Protein Kinase

Reilly

Guarino

1994

Journal of General Virology

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Open reading frame (ORF) 9 of the EcoRI I fragment of the baculovirus Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) genome encodes a protein, PK-1, which has strong similarity to serine-threonine protein kinases. The published sequence of the pk-1 gene contains an error that, when corrected, extends the ORF by 228 nucleotides. Transcription of pk-1 was first detectable by primer extension at 12 h post-infection, accumulating to high levels during the very late phase of infection. PK-1 produced in rabbit reticulocyte lysates was able to phosphorylate histone H1. Together, our results suggest that ORF 9 encodes a protein kinase that is expressed during the beginning of the late and throughout the very late phases of AcMNPV infection.

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“…The AcMNPV genome also contains two iap-like sequences, designated iap1 and iap2 (Braunagel et al, 1993 ;Crook et al, 1993 ;Ayres et al, 1994). The Cp-iap, Op-iap and Ac-iap1 predicted protein sequences contain no recognizable signal sequences, transmembrane domains or nuclear localization signals.…”

Section: Introductionmentioning

confidence: 99%

In vitro host range of Autographa californica nucleopolyhedrovirus recombinants lacking functional p35, iap1 or iap2.

Griffiths¹,

Barnett

Ayres³

et al. 1999

Journal of General Virology

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We have examined the host range in different insect cell lines of Autographa californica nucleopolyhedrovirus (AcMNPV) recombinants lacking p35, iap1 or iap2. These genes encode, or are predicted to encode, anti-apoptotic proteins. Abrogation of p35 reduced the ability of AcMNPV to replicate in permissive cell lines derived from Spodoptera frugiperda insects by inducing apoptosis. In semi-permissive cell lines, such as Lymantria dispar and Spodoptera littoralis cells, we observed cytopathic effects after infection with AcMNPV but little virus production. Infection of these cells by AcMNPV lacking p35 resulted in apoptosis. However, p35-deficient viruses were still able to replicate normally in Trichoplusia ni, Mamestra brassicae and Panolis flammea cell lines. Disruption of AcMNPV iap1 and iap2 was found not to affect virus replication in any of the cell lines. It was also possible to disrupt both iap1 and iap2 in the same virus without loss of infectivity. A virus without iap1 and p35 demonstrated identical growth characteristics and host range to a virus lacking p35. We conclude that in cells which respond to AcMNPV infection by initiating programmed cell death, the p35 gene product alone is sufficient to inhibit apoptosis. Removal of iap1 or iap2 has no effect on virus replication, even in cell lines which do not undergo apoptosis in response to AcMNPV infection. Our results with two semi-permissive cell lines further indicate that whilst p35 is important in blocking block apoptosis, other factors are involved in restricting AcMNPV replication within these cells.

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Sequence, genomic organization of the EcoRI-A fragment of Autographa californica nuclear polyhedrosis virus, and identification of a viral-encoded protein resembling the outer capsid protein VP8 of rotavirus

Cited by 15 publications

References 18 publications

Anti- and pro-apoptotic activities of baculovirus and Drosophila IAPs in an insect cell line

Anti- and pro-apoptotic activities of baculovirus and Drosophila IAPs in an insect cell line

The pk-1 Gene of Autographa Californica Multinucleocapsid Nuclear Polyhedrosis Virus Encodes a Protein Kinase

In vitro host range of Autographa californica nucleopolyhedrovirus recombinants lacking functional p35, iap1 or iap2.

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