Sequence homologies between bovine papillomavirus genomes mapped by a novel low-stringency heteroduplex method

Coggins, Lesley W.; Ma, Jiqing; Slater, Alison; Campo, M. Saveria

doi:10.1016/0042-6822(85)90398-8

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…Analysis of the deduced amino acid sequences shows that the areas of greater homology between BPV-4 and BPV-1 are in E1 and L1 (Table 4), as previously concluded for DNA from heteroduplex data (Coggins et al, 1985), and that BPV-4 is evolutionarily closer to the epitheliotropic human and rabbit papillomaviruses than to BPV-1. Therefore, it appears that papillomaviruses evolve subject to constraints imposed by the host cell.…”

Section: Amino Acid Sequence Homology Between the Polypeptides Of Bp mentioning

confidence: 61%

“…The subgroup B viruses are less well characterized. Among their features are their small genome and their lack of cross-reactivity with the subgroup A viruses (but see Coggins et al, 1985). BPV-4, the most extensively studied virus in subgroup B, causes papillomas of the upper alimentary canal (Campo et al, 1980), which provide a focus for malignant transformation in animals eating bracken fern (Jarrett et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

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The Nucleotide Sequence and Genome Organization of Bovine Papillomavirus Type 4

1987

Journal of General Virology

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SUMMARYThe nucleotide sequence of bovine papillomavirus type 4 (BPV-4) was determined. The viral genome is 7261 base pairs long. Several overlapping open reading frames (ORFs) have been identified both on the basis of amino acid comparison with other papiUomaviruses and on their transcriptional pattern. Eight early ORFs (E 1 to 8) were recognized, coding for DNA replication and cell transformation functions, and three late ORFs (L1 to 3), coding for structural proteins. Like the E50RF of human papillomavirus type 6 the E50RF of BPV-4 is discontinuous. Unlike other papillomaviruses, the non-coding region upstream of the early ORFs (ncr-1) is short (385 base pairs), but there is another non-coding region (ncr-2) of nearly 500 base pairs between the L2 and L10RFs. Most of the putative regulatory sites are located in the ncr-1, although potential controlling elements are also found in other parts of the genome. Polyadenylation sites are present at the 3' end of both the early and the late transcription units. Comparison between the polypeptides of BPV-4 and other papillomaviruses showed that BPV-4 is evolutionarily closer to the epitheliotropic human and rabbit viruses than to BPV-1.

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Section: Amino Acid Sequence Homology Between the Polypeptides Of Bp mentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

The Nucleotide Sequence and Genome Organization of Bovine Papillomavirus Type 4

1987

Journal of General Virology

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“…From the hybridization pattern, these transformants were found to contain viral D N A identical to BPV-lb, as found in ID14 cells (data not shown) (Chen et al, 1982;Coggins et al, 1985). The transformants were analysed for the degree of expression of the transformed phenotype, i.e.…”

mentioning

confidence: 99%

Characterization of Bovine Papillomavirus Type 1-transformed Clones which Show Distinct Transformed Phenotypes

Tada

Sekine

Yamamoto

et al. 1989

Journal of General Virology

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SUMMARYSeventeen independent cell clones were isolated from C127 cells transformed by bovine papillomavirus type 1 (BPV-1). Transformants showed differing degrees of expression of the transformed phenotype as monitored by saturation density, doubling time, growth in medium with a low serum concentration and colony-forming efficiency in soft agar. The degree of expression of the transformed phenotype did not correlate with either the BPV-1 copy number or levels of BPV-l-specific RNA in the transformed cell clones. A characteristic transformed cell clone, Tlc, showed the lowest degree of expression of the transformed phenotype but contained the highest copy number ofBPV-1 DNA and the highest level of BPV-l-specific mRNA. When we analysed different transformants by two-dimensional gel electrophoresis, we found that a set of six proteins changed quantitatively. Changes in the expression of these proteins were most consistent in clones expressing the greatest number of parameters of transformation, e.g. clone T4a. These data indicate that changes in the expression of cellular genes may correlate with the degree of expression of the transformed phenotype.

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Papillomavirus Infection in Cattle: Viral and Chemical Cofactors in Naturally Occurring and Experimentally Induced Tumours

Campo

Jarrett

2007

Ciba Foundation Symposium 120 ‐ Papillomaviruses

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Sequence homologies between bovine papillomavirus genomes mapped by a novel low-stringency heteroduplex method

Cited by 8 publications

References 17 publications

The Nucleotide Sequence and Genome Organization of Bovine Papillomavirus Type 4

The Nucleotide Sequence and Genome Organization of Bovine Papillomavirus Type 4

Characterization of Bovine Papillomavirus Type 1-transformed Clones which Show Distinct Transformed Phenotypes

Papillomavirus Infection in Cattle: Viral and Chemical Cofactors in Naturally Occurring and Experimentally Induced Tumours

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