Sequence homology between avian and human adenoviruses

Aleström, Peter; Stenlund, A; Li, P; Bellett, A. J. D.; Pettersson, Ulf

doi:10.1128/jvi.42.1.306-310.1982

Cited by 34 publications

(8 citation statements)

References 18 publications

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“…The complete CELO virus sequence was published only 2 years ago (13). Information on its homology with human adenoviruses has been based solely on data bank analysis and computer comparisons (3,4,13,33). The present study provides essential information on transcription of the predicted ORFs.…”

Section: Discussionmentioning

confidence: 97%

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Transcriptional Organization of the Avian Adenovirus CELO

Payet¹,

Arnauld²,

Picault³

et al. 1998

J. Virol.

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A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs). This study, based on analysis of the transcriptional products of CELO in vitro, confirmed the presence of RNAs for 26 of these 38 ORFs. All of the results were obtained by cDNA isolation or specific reverse transcriptase PCR. Observation of ORF transcription kinetics postinfection revealed the existence of early and late expression, with the earliest starting at 2 h postinfection. The 5′ untranslated regions of some RNAs were also studied, and this revealed the existence of a bipartite leader sequence, comparable in structure to the tripartite leader of mastadenovirus. The leader most probably involved in transcriptional activity was observed in most of the structural protein genes of the CELO virus genome. This suggests some homology in transcriptional organization between the avian adenovirus CELO and known mastadenoviruses such as human adenovirus.

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Section: Discussionmentioning

confidence: 97%

“…The CELO virus genome is a doublestranded DNA molecule 43,804 bp in length (13) (GenBank accession no. U46933) and has inverted terminal repeats (ITRs) that are shorter than mastadenovirus ITRs (3,4,51,53). As in human adenovirus, a terminal protein (TP) is covalently attached to the 5Ј terminus of the CELO virus genome (33).…”

mentioning

confidence: 99%

Transcriptional Organization of the Avian Adenovirus CELO

Payet¹,

Arnauld²,

Picault³

et al. 1998

J. Virol.

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“…the evolution of this protein could have been dictated by its interactions with other highly conserved virion components, e.g., hexon and the viral genome. It is noteworthy that homology is not detected in this region between chicken embryo lethal orphan virus and HAV-2 genomes (1).…”

Section: Rimentioning

confidence: 98%

“…between chicken embryo lethal orphan virus and HAV-2 is limited to the carboxy terminus of the hexon gene (1). the cross-hybridization between BAV-7 and HAV-2 was detected in most parts of the hexon coding sequence.…”

mentioning

confidence: 94%

Restriction analysis and homology studies of the bovine adenovirus 7 genome

Hu¹,

Battles²,

Potts³

1984

J Virol

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We located the cleavage sites for restriction endonucleases EcoRI, HindIII, and BamHI on the genome of bovine adenovirus 7. Cross-hybridization at reduced stringency revealed two regions of homology shared by the DNA of human adenovirus 2 and bovine adenoviruses 7 and 3. These regions correspond to the hexon and the IVa2 protein genes of the human adenovirus. Another region of homology shared only by the human adenovirus and bovine adenovirus 7 corresponded to the penton or the polypeptide IIIa genes. These results allowed us to align the restriction map of bovine adenovirus 7 with respect to the other adenoviruses.

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“…As an approach to establishing whether HCMV DB140 and SCMV DB129 are homologs to the HSV DBP, we used the cloned HSV-1 ICP8 gene to search for cross-hybridizing sequences in the CMV genome. This general strategy has been useful in many systems (1,19,21). The results presented in this report show that the cloned HSV ICP8 gene cross-hybridized with a specific region of the HCMV genome.…”

mentioning

confidence: 99%

Location, transcript analysis, and partial nucleotide sequence of the cytomegalovirus gene encoding an early DNA-binding protein with similarities to ICP8 of herpes simplex virus type 1

Anders

Gibson

1988

J Virol

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The results presented here locate the gene encoding an early, nonvirion, single-stranded DNA-binding protein of human and simian strains of cytomegalovirus (CMV) [HCMV(Towne) DB140 and SCMV(Colburn) DB129, respectively] and provide additional evidence that this protein is the CMV homolog of the herpes simplex virus type 1 (HSV-1) major DNA-binding protein (ICP8), as proposed earlier (D. G. Anders, A. Irmiere, and W. Gibson, J. Virol. 58:253-262). The ICP8 gene was used as a probe in Southern analyses done at moderate stringency as an approach to locating similar sequences in the CMV genome. The BamHI K and EcoRI V fragments from the center of the long unique segment of HCMV(Towne) hybridized with the ICP8 probe and were in turn used to identify corresponding sequences in the EcoRI D fragment of SCMV(Colburn). RNA prepared from SCMV(Colburn)-infected cells directed the in vitro synthesis of DB129. If the RNA was first hybridized with the cloned 12.5-kilobase EcoRI D fragment, in vitro synthesis of DB129 was specifically inhibited. Additional hybrid-arrested in vitro translation experiments with subclones spanning the EcoRI D fragment demonstrated that the DB129 gene is located in the left half of that fragment, approximately bisected by a SalI site. RNA analyses identified 3.9-, 8.9-, and 10.0-kilobase RNA species expressed from this region. A partial nucleotide sequence of the Colburn region mapping within the boundaries of the 3.9-kilobase transcript, suspected to be the primary coding species, showed significant sequence similarity to the major DNA-binding protein gene homolog identified in B95-8 Epstein-Barr virus.

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Sequence homology between avian and human adenoviruses

Cited by 34 publications

References 18 publications

Transcriptional Organization of the Avian Adenovirus CELO

Transcriptional Organization of the Avian Adenovirus CELO

Restriction analysis and homology studies of the bovine adenovirus 7 genome

Location, transcript analysis, and partial nucleotide sequence of the cytomegalovirus gene encoding an early DNA-binding protein with similarities to ICP8 of herpes simplex virus type 1

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