Sequence of 3,687 nudeotides from the 3′ end of Sendai virus genome RNA and the predicted amino add sequences of viral NP, P and C proteins

Abstract

The sequence of 3,687 nucleotides from the 3' end of the Sendai virus genome (Z strain) was determined by a molecular cloning technique followed by rapid sequence analysis. Two large open reading frames, one consisting of 1,572 nucleotides and the other of 1,704 nucleotides, were observed in the region, that is OP-1 and OP-2 from the 3' end of the genome. The amino acid sequences of the gene products were predicted from the observed sequence. Determination of amino acid compositions of viral proteins, P, HN, Fo, NP and M, led us to conclude that NP and P are the gene products of OP-1 and OP-2, respectively. An additional open reading frame consisting of 612 nucleotides (OP-3) was discovered in the 3' most proximal region of OP-2. The predicted product of OP-3 was considered to be viral non-structural protein C. The leader sequence of 51 nucleotides at the 3' terminal of the genome and consensus sequences at 3' and 5' ends of each gene for proteins NP and P were identified.

The STAT2 Activation Process Is a Crucial Target of Sendai Virus C Protein for the Blockade of Alpha Interferon Signaling

“…DNA fragments encoding D1 (amino acids [aa] 85 to 204), D2 (aa 127 to 204), and D3 (aa 30 to 126), in addition to C (aa 1 to 204), Y1 (aa 24 to 204), and Y2 (aa 30 to 204) (Fig. 1A), were amplified by PCR by using the cDNA clone H-33 (38) 728 TT ACTCTTGCACTATGTG-3Ј) were used for the PCRs for C, Y1, Y2, D1, and D2. Italics indicate nucleotide sequences that are not involved in ORFs.…”

Sendai virus C proteins are categorically nonessential gene products but silencing their expression severely impairs viral replication and pathogenesis

“…The mutations in the complementary primers are shown in lower-case letters. Nucleotide numbering was done according to Shioda et al (1983). The two fragments were combined in the subsequent fusion reaction in which the overlapping ends annealed, allowing the 3 0 overlap of each strand to serve as primers for 3 0 extension of the complementary strand.…”

Molecular Cloning and Sequence Analysis of the Mumps Virus Gene Encoding the P Protein: Mumps Virus P Gene Is Monocistronic

“…The previous studies are not fully in accord, however, as to whether the non-structural proteins are related to the P protein or not (Herder & Compans, 1982;Merz et al, 1983;Simpson et al, 1984). It is known that many paramyxoviruses such as Sendal virus (Giorgi et aL, 1983 ;Shioda et al, 1983), human parainfluenza virus 3 (PF-3) (Galinski et al, 1986;Luk et al, 1986;Spriggs & Collins, 1986), measles virus (Bellini et al, 1985) and canine distemper virus (Barrett et al, 1985) utilize different reading frames present in the P protein mRNA to code for the non-structural C proteins. By sequencing cloned cDNA of the P gene region, we attempted to determine whether a bicistronic coding strategy is also used in the mumps virus P gene.…”