Sequence of a cDNA encoding human keratin No 10 selected according to structural homologies of keratins and their tissue-specific expression

Darmon, Michel; Sémat, Alix; Darmon, Michèle; Vasseur, Marc

doi:10.1007/bf00444680

Cited by 17 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned earlier, keratin 10 was expressed in biphasic synovial sarcoma cases, while dynactin-1 and the lumican precursor were expressed in monophasic cases, and annexin 2 was expressed in both subtypes. Keratin 10 has been identified as being a structural constituent of the epidermis and as promoting the epidermis differentiation of squamous carcinomas [46][47][48][49]. Dynactin-1 facilitates mitosis and nervous system development [50][51][52][53][54][55].…”

Section: Discussionmentioning

confidence: 99%

GST‐P1 as a histological biomarker of synovial sarcoma revealed by proteomics

Suehara

Kikuta

Nakayama

et al. 2009

Proteomics Clinical Apps

View full text Add to dashboard Cite

Synovial sarcoma have two histological subtypes, biphasic and monophasic, defined respectively by the presence or absence of glandular epithelial differentiation. To develop histological biomarkers for synovial sarcoma subtypes, we examined the proteomic profile using two-dimensional difference gel electrophoresis. We identified 29 protein spots whose intensity was statistically different between the monophasic (15 cases) and biphasic (9 cases) subtypes (p ,0.01). Mass spectrometric protein identification demonstrated that these 29 spots corresponded to 24 distinct gene products involved in cytoskeletal organization, trsnscription/trsnslation, protein/ collagen binding, and ion transport, as well as structural constituents of the epidermis. Two of the 29 spots derived from glutathione S-transferase P (GST-P1) had higher intensity in biphasic type. Immunohistochemistry on additional 42 synovial sarcoma cases revealed that positive expression of GST-P1 was observed in 10 of 12 biphasic (83.3%), in 4 of 27 monophasic (14.8%) and in 1 of 3 poorly differentiated synovial sarcomas (p = 0.0002). Among the clinico-pathological parameters examined, GST-P1 expression significantly correlated only with the histological subtype. GST-P1 had more discriminative power than the status of fusion genes SYT-SSX1 and SYT-SSX2, previously reported to be correlated with the histological subtype. These results establish GST-P1 as a histological biomarker candidate for synovial sarcoma differentiation into subtypes.

show abstract

Section: Discussionmentioning

confidence: 99%

GST‐P1 as a histological biomarker of synovial sarcoma revealed by proteomics

Suehara

Kikuta

Nakayama

et al. 2009

Proteomics Clinical Apps

View full text Add to dashboard Cite

show abstract

“…Amino acids that are identical to the h,m K18 sequence are represented by bold dots. The indicated sequences are for m,hK18 (25); mK15 (26); m,hK19 (27); hK9 (28); hK10 (29); mK12 (30); hK13 (31); hK14 (32); hK15 (33); hK16 (34); hK17 (35); and hK20 (36).…”

Section: Discussionmentioning

confidence: 99%

Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Ku¹,

Wright²,

Terrault³

et al. 1997

J. Clin. Invest.

109

View full text Add to dashboard Cite

Mutations in 11 of the more than 20 keratin intermediate filaments cause several epidermal and oral associated diseases. No disease-associated mutations have been described in keratin 8 or 18 (K8/18) which are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. However, transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity. Also, ectopic expression of epidermal K14 in mouse liver results in chronic hepatitis, and disruption of mouse K8 leads to embryo lethality with extensive liver hemorrhage. We tested if patients with liver disease of unknown cause may harbor mutations in K18. We describe a his127 → leu (H127L) K18 mutation in a patient with cryptogenic cirrhosis that is germline transmitted. The K18 H127L isolated from the liver explant, or after expression in bacteria, showed an altered migration on two-dimensional gel analysis as compared with normal human liver or bacterially expressed K18.

show abstract

“…1985Zhou et al, 1988. Rieger and Franke, 1988, Darmon et al, 1987Tkachenko et al, 1992).Here we present the results of in situ hybridization, which localize K51 (KRT10L) to human chromosome 9, rat chromo some 3, pig chromosome 6, cattle chromosome 18, and mink chromosome 1. …”

mentioning

confidence: 99%