Sequence of an intestinal cDNA encoding human motilin precursor

Seino, Yutaka; Tanaka, Ken; Takeda, Jun; Takahashi, Hideo; Mitani, Tadao; Kurono, Masayasu; Kayano, Toshiaki; Koh, Gyohan; Fukumoto, Hirofumi; Yano, Hideki; Fujita, Jun; Inagaki, Nobuya; Yamada, Yoshio; Imura, Hiroo

doi:10.1016/0014-5793(87)80512-4

Cited by 42 publications

(13 citation statements)

References 14 publications

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“…15,16) The amino acid sequence of human motilin remained unclear for a long time after the discovery of porcine motilin, but in 1987 was found to be identical to that of porcine motilin. 17) We thought that motilin may be useful for the treatment of gastroparalysis due to the pharmacological actions mentioned above. Therefore, various methods of synthesizing of motilin analogues were investigated in our laboratory, and a new high-yield, low-cost method for synthesis of a new human motilin analogue, SK-896 ([Leu 13 ]motilin-Hse), was established using recombinant DNA techniques.…”

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confidence: 99%

Effects of SK-896, a New Human Motilin Analogue ([Leu13]motilin-Hse), on Postoperative Ileus in Dogs after Laparotomy.

Furuta

Takeda

Nakayama

et al. 2002

Biological & Pharmaceutical Bulletin

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The effects of SK-896, a new human motilin analogue ([Leu 13 ]motilin-Hse), on digestive tract motility in postoperative ileus were evaluated in a dog model of ileus after laparotomy. SK-896 was intravenously administered at 0.17, 0.33 and 0.67 m mg/kg starting soon after operation and then at 6-h intervals, for a total of 9 times. SK-896 progressively, dose-dependently and significantly increased the duodenal motility from 1 h after operation. The recovery time of the gastrointestinal-interdigestive migrating complex (GI-IMC) activity, which is an indicator of normal gastrointestinal tract activity after laparotomy, was 56.5؎5.0 h in the control group. SK-896 significantly shortened this recovery time. On the other hand, the plasma SK-896 concentrations declined diexponentially after administration, and can be described by a linear pharmacokinetic model within the dose range used. In addition, the pharmacokinetics of SK-896 did not change significantly at any postoperative time. There was no correlation between the plasma SK-896 concentrations and the intensity of duodenal motility, because the activity in the duodenum decreased transiently 13 h after laparotomy and increased with time thereafter. The changes in the activity are considered to reflect the progressive changes in the state of ileus. In conclusion, SK-896 increased the duodenal motility significantly, shortening the recovery time of GI-IMC-like activity in dogs with post-laparotomy ileus. Therefore, it is expected from these results that SK-896 would be useful and effective for the treatment of gastroparalysis after abdominal surgery.

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mentioning

confidence: 99%

Effects of SK-896, a New Human Motilin Analogue ([Leu13]motilin-Hse), on Postoperative Ileus in Dogs after Laparotomy.

Furuta

Takeda

Nakayama

et al. 2002

Biological & Pharmaceutical Bulletin

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“…A size-fractionated human genomic library was constructed. A library of approximately 2 X 105 independently derived bacteriophage EMBL4 clones consisting of large (15-20 kb) fragments of human DNA covalently joined to the bacteriophage EMBL4 was prepared, as previously described [8]. In brief, the patient's leukocyte DNA was subjected to nonlimit digestion with the restriction endonuclease EcoR I, and the products were sizefractionated by gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Hind III Site Causing Proinsulin Kyoto and Pst I Site Polymorphism of the Insulin Gene in Japanese: Its Lack of Association with Either IDDM or NIDDM.

et al. 1994

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Abstract. The gene encoding Proinsulin Kyoto has been isolated and characterized by DNA sequencing, indicating that the molecular basis of the disorder is a G-T point mutation in the insulin gene which creates a Hind III site. In addition, in the 3'-untranslated region of the mutant insulin gene, a Pst I site negative, a type allele was found, and in the normal gene, a Pst I site positive, f3 type allele was found. In order to clarify the frequency of the mutation and to determine whether this mutation is associated with diabetes mellitus or not, we have investigated Hind III polymorphism in 91 normal Japanese subjects and patients with IDDM and NIDDM. No cases with the Proinsulin Kyoto gene were found among the subjects examined. Secondly, to determine whether this a type allele is associated with DM in Japanese, we investigated Pst I polymorphism in the same subjects. The frequencies of the a type and 13 type alleles were 92% and 8%, respectively. No significant difference in genotypic frequency was found among normal, NIDDM, and IDDM. We conclude that the Proinsulin Kyoto gene is not a common cause of DM and the occurrence of the a type insulin gene in Japanese diabetes is more frequent than in other races, so this Pst I polymorphism is not a marker for diabetes mellitus in Japanese. Key words: ProinsulinKyoto, NIDDM, Insulin gene, RFLP, Pst I site.(Endocrine journal 41: [71][72][73][74]1994) A NEW TYPE OF hyperproinsulinemia has recently been reported by our laboratory in a 65-yrold non-obese Japanese man who developed NIDDM [1]. A family study revealed that his son and daughter also showed signs of fasting hyperinsulinemia.Analysis of their sera by reverse phase high performance liquid chromatography demonstrated a minor peak of human insulin (5%) and a major peak of Proinsulin-like material (95%). Amplification and sequencing of the products of the insulin gene by polymerase chain reac-

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“…cDNA encoding the motilin precursor has previously been isolated and sequenced from man [8,9], pig [10], rabbit [11], sheep [12], monkey [13], dog, chicken, horse, and cow [6]. In every species the precursor consists of a 25 amino acid signal peptide, 22 amino acid motilin itself and a MAP of variable length.…”

Section: Homology With the Motilin Precursors From Other Speciesmentioning

confidence: 99%

Identification and expression of the motilin precursor in the guinea pig

et al. 2001

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Motilin has never been isolated from rodents, the most frequently used laboratory animals, despite several attempts. We have isolated and sequenced the motilin precursor from duodenal mucosa of guinea pig (GenBank accession number AF323752) and studied its expression in several tissues. The percent homology with human motilin is the lowest yet observed due to several unique substitutions in the C-terminal end. As expected, the precursor was present in the gut mucosa with the exception of the gastric corpus. It was also present in medulla oblongata, nucleus of the solitary tract, hypophysis, spinal cord, hypothalamus, and cerebellum but not in the cerebral cortex. For the first time we demonstrated motilin expression in the thyroid. ß

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Sequence of an intestinal cDNA encoding human motilin precursor

Cited by 42 publications

References 14 publications

Effects of SK-896, a New Human Motilin Analogue ([Leu13]motilin-Hse), on Postoperative Ileus in Dogs after Laparotomy.

Effects of SK-896, a New Human Motilin Analogue ([Leu13]motilin-Hse), on Postoperative Ileus in Dogs after Laparotomy.

Hind III Site Causing Proinsulin Kyoto and Pst I Site Polymorphism of the Insulin Gene in Japanese: Its Lack of Association with Either IDDM or NIDDM.

Identification and expression of the motilin precursor in the guinea pig

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