Sequence of cDNA coding for human keratin 19

Stasiak, Pauline C.; Lane, E B

doi:10.1093/nar/15.23.10058

Cited by 43 publications

(19 citation statements)

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“…However, the keratin 19 gene expression in non-epithelial tissues seems to be much more restricted compared to keratin 8 and 18 gene expression (Traweek et al, 1993). The keratin 19 protein structure is also unusual, as shown by cDNA sequencing of human (Bader et al, 1988;Eckert, 1988;Stasiak and Lane, 1987), bovine (Bader et al, 1986), murine (Lussier et al, 1990) and potoroo (this study) keratin 19. Its small size is due to the absence of a long nonhelical tail domain, which would normally be located after the consensus termination peptide at the end of the central a-helical rod domain.…”

supporting

confidence: 50%

“…The isolation using monoclonal antibodies of cDNA clones coding for keratin 19 from a commercial i g t l l expression library of human placenta has been described previously (Stasiak and Lane, 1987;Stasiak et al, 1989). Clone PSE20 [I203 bp, including full-length coding region, 10-bp 5' and 159-bp 3' untranslated region (UTR)] was excised from &I1 with EcoRI and subcloned into the EcoRI site of the plasmid expression vector pUR292 (Ruther and Muller-Hill, 1983) for protein expression.…”

Section: Methodsmentioning

confidence: 99%

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Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

Böttger

Stasiak

Harrison

et al. 1995

European Journal of Biochemistry

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To generate tools for monitoring processing and folding in keratin intermediate filaments, a group of monoclonal antibodies reacting with the intermediate filament protein keratin 19 were studied using different approaches to define the structure and localization of their epitopes. The binding pattern to bacterially expressed human keratin 19 fragments allowed the definition of minimal amino acid sequences required for antibody binding. The screening of overlapping 15-residue peptides confirmed and further specified the epitope locations for a subset of the tested antibodies. In addition, the epitope of an antibody with apparent species-restricted specificity (LE64) was revealed by isolating and characterizing a fulllength keratin 19 clone from a PtK2 cDNA library. Taken together with species cross-reactivity of individual antibodies and sequence information obtained by probing a phage display library, specific amino acid residues could be highlighted as likely to be involved in the antibody binding.

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supporting

confidence: 50%

Section: Methodsmentioning

confidence: 99%

Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

Böttger

Stasiak

Harrison

et al. 1995

European Journal of Biochemistry

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“…The dilution series of normal control cDNAs served to provide a standard curve and allowed the quantification of the PCR-produced yield for all samples. A well-adapted set of primers was used that correspond to the coding sequences of human ␤-catenin (X87838; sense: 5Ј-GC-CGGCTATTGTAGAAGCTG-3Ј; antisense: 5Ј-TGATGTCTTC-CCTGTCACCA-3Ј), human cytokeratin 19 (NM002276; sense: 5Ј-TTTGAGACGGAACAGGCTCT-3Ј; antisense: 5Ј-TCTTCCA-AGGCAGCTTTCAT-3Ј), human desmoglein-2 (Z26317; sense: 5Ј-CATGACTCCTATGTGGGCCT-3Ј; antisense: 5Ј-GCAGGC-TTCTGTGTTCTTCC-3Ј), human E-cadherin (Z13009; sense: 5Ј-GAACGCATTGCCACATACAC-3Ј; antisense: 5Ј-GAAGGT-CAGCAGCTTGAACC-3Ј), and human occludin (NM002538; sense: 5Ј-TATGGCTATGGCTACGGAGG-3Ј; antisense: 5Ј-TG-TGTTCCTTGTCCCACAAA-3Ј) (1,4,23,24,35,46). All primers were synthesized by Life Technologies.…”

Section: Methodsmentioning

confidence: 99%

Inflammatory bowel disease is associated with changes of enterocytic junctions

Gaßler

Rohr

Schneider³

et al. 2001

American Journal of Physiology-Gastrointestinal and Liver Physi

329

254

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Changes of the intestinal mucosal barrier are considered to play a role in the pathogenesis of inflammatory bowel disease (IBD). Our experiments were designed to identify dysregulation of epithelial junctional molecules in the IBD intestinum and to address whether altered expression of these molecules is a primary event in IBD or a phenomenon secondary to the inflammatory process. Noninflamed and inactively and actively inflamed mucosal tissues from patients with ulcerative colitis or Crohn's disease as well as tissues from control subjects were analyzed for the expression of junctional molecules by different methods. Marked downregulation of junctional proteins and their respective mRNAs was observed in actively inflamed IBD tissues. In IBD tissues with inactive inflammation, only a few junctional molecules such as E-cadherin and α-catenin were affected, whereas expression of desmosomal or tight junction-associated proteins appeared almost unchanged. In noninflamed IBD tissues, junctional protein expression was not different from that seen in normal control subjects. In IBD, downregulation of junctional molecule expression is apparently associated with the inflammatory process and does not likely represent a primary phenomenon.

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“…Total RNA (1 ,ug) was denatured together with oligo-dT primer (50 pmol) and kinase insert domain-containing receptor (KDR)]. PCR primers were 5'-GAAGTGGTGAAGTTCATGGATGTC-3' and 5'-CGATCGTTCTGTATCAGTCTTTCC-3' for VEGF cDNA, according to the VEGF gene structure (Tischer et al, 1991); 5'-GAGAATTCACTATGGAAGATCTGATTTCTTACAGT-3' and 5'-GAGCATGCGGTAAAATACACATGTGCTTCTAG-3' for flt-1; 5'-AGCCATTCATCAACAAGCCT-3' and 5'-GGCAACA-GCTGGATGTCATA-3' for flt-4; 5'-TATAGATGGTGTAACC-CGGA-3' and 5'-TTTGTCACTGAGACAGCTTGG-3' for KDR; and 5'-AGGTGGATTCCGCTCCGGGCA-3' and 5'-AThTTCCT-GTCCCTCGAGCA-3' for keratin 19 (Stasiak et al, 1987). For Southern blot analysis, the PCR products were electrophoresed through a 1.0% agarose gel and were transferred to a nylon membrane filter.…”

Section: Rt-pcr Analysismentioning

confidence: 99%

Vascular endothelial growth factor and lymph node metastasis in primary lung cancer

Ohta

Watanabe²,

Murakami³

et al. 1997

Br J Cancer

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Summary The relationship between vascular endothelial growth factor (VEGF) and lymph node metastasis was studied in 90 cases of primary lung cancer without distant metastasis. As a result of quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis, the VEGF121 mRNA expression levels in lung cancer tissues with nodal metastasis (n = 35) were higher than in those without nodal metastasis (n = 55). However, no significant difference could be found in VEGF121 mRNA expression levels as stratified by tumour size (Ti NOMO vs T2NOMO). Simultaneously, ten lymph nodes (four node positive and six node negative) together with the corresponding primary lung tumours and adjacent normal lung tissue, were studied for VEGF expression. The VEGF mRNA expression in metastatic lymph nodes was intense in three out of the four cases examined. Further, while VEGF expression levels in metastatic lymph nodes were conspicuously higher than those for the primary site, all its expression levels in non-metastatic nodes were inferior to those of the primary tumours. Except for macrophages, the VEGF antigen was identified mainly in the cytoplasm of metastatic cancer cells and the endothelial cells of blood or lymphatic vessels in lymph nodes. Although the detailed mechanisms and the significance of strong VEGF expressions in metastatic lymph nodes are still unknown, these data are consistent with a model whereby VEGF increases the opportunity for nodal metastasis through neoblood and lymphatic vessels.

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Sequence of cDNA coding for human keratin 19

Cited by 43 publications

References 4 publications

Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

Inflammatory bowel disease is associated with changes of enterocytic junctions

Vascular endothelial growth factor and lymph node metastasis in primary lung cancer

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