Sequence of GDF 8 (Myostatin) Gene in<i>Bubalus Bubalis</i>

Tantia, M. S.; Vijh, R. K.; Mishra, Bina; Kumar, S T Bharani

doi:10.1080/10495390601115526

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…To date, in all vertebrates, it has been described that the genomic organization of the myostatin gene has three exons of similar size separated by two introns and whose size is conserved in birds and mammals. As confirmed by Tantia et al [21]. comparing the size of the exons as well as the introns of the Bos Indicus, Bos taurus, Bubalus bubalis, Ovis aries and Homo sapiens.…”

Section: Discussionmentioning

confidence: 63%

Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype

et al. 2021

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Since the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.

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Section: Discussionmentioning

confidence: 63%

Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype

et al. 2021

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Myostatin gene polymorphisms do not determine muscle hypertrophy in buffaloes: A case report

Santos Alves,

Tavares Rolim-Filho,

Tonhati

et al. 2024

Buffalo Bull.

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The aim of the study was to detect variations in the exonic and intronic regions of the myostatin gene in buffaloes with different body conformations. The regions of the gene were analyzed using the PCR-sequencing technique comparing an animal with muscle hypertrophy and ten animals with normal musculature. The hypertrophied buffalo sequence was compared to the others and no exclusive genotype was identified. It suggests that the found MSTN variants are not singly associated to muscle hypertrophy in buffaloes.

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Sequence of GDF 8 (Myostatin) Gene inBubalus Bubalis

Cited by 2 publications

References 7 publications

Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype

Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype

Myostatin gene polymorphisms do not determine muscle hypertrophy in buffaloes: A case report

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