Sequence of the 3'-part of the RNA of ryegrass mosaic virus, a potyvirus

Schubert, Jörg; Rabenstein, Frank; Proll, E.

doi:10.1051/agro:19950711

Cited by 10 publications

(7 citation statements)

References 7 publications

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“…The numerous economically important aphid transmitted potyviruses share evolutionary relationship with fungus transmitted bymoviruses [22,23]. The studies on molecular characterization and virus vector relationships resulted many revisions and there were proposals to assign genus name to the unassigned Potyviridae members viz., whitefly transmitted SpMMV of Ipomovirus [24,25], mite transmitted RGMV [26], AgMV and HoMV [27] of Rymovirus [28], BrSMV [29], WSMV [30,31] of Tritimovirus [25]. The revision and proposals were reflected in the subsequent ICTV report [32].…”

Section: Discussionmentioning

confidence: 99%

Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane

2008

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Sixty-three sugarcane leaf samples were collected from fifty-eight sugarcane varieties, evolved from eleven major sugarcane growing states in India, Australia, South Africa and USA. In RT-PCR, using gene specific primers for sugarcane streak mosaic virus (SCSMV)-CP, 58 of 63 sugarcane samples were found positive to the virus infection and rest of the five samples were negative. Partial CP gene sequences of 42 SCSMV isolates including an isolate from aphid colony (Melanaphis indosacchari) infested on sugarcane variety from this study were characterized after cloning and sequencing for selective isolates represented by at least one isolate from each location. The new sequences identified in the study were named as SCSMV-CB isolates. Fifty two sequences including the 10 database sequences (complete CP cds) deposited earlier from this institute were compared with each other as well as GenBank database sequences of Potyviridae members viz., Rymovirus, Potyvirus, Ipomovirus, Tritimovirus and eight sequences of SCSMV reported from elsewhere. Among the SCSMV-CB isolates sequenced in the study, 85.7-100% (nucleotide) and 89.9-100% (amino acid) sequence identities were observed and with the other data base sequences of SCSMV, the respective identities were 82.2-97.5 and 89.7-98.6%. Grouping of the isolates by the maximum likelihood with molecular clock model, distributed 60 SCSMV sequences including the eight database sequences deposited by other SCSMV working groups from India and USA in 16 different phylogenetic groups. Although the isolates of SCSMV were relatively close to Ipomovirus and Tritimovirus, they were sandwiched between Rymovirus and Ipomovirus. The sequence comparison and phylogenetic studies revealed that the relatedness of SCSMV with the potyviral related genera was comparatively low to consider it as a member of earlier described potyviral genera, hence the genus "Susmovirus" (sugarcane streak mosaic virus) has been proposed, with SCSMV as the sole species to be included. The 52 SCSMV-CB isolates from this institute were distributed in 14 phylogenetic groups and the grouping pattern revealed that the virus isolates could not be grouped based on geographical origin of the host varieties or longevity of the host variety.

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Section: Discussionmentioning

confidence: 99%

Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane

2008

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“…Previous phylogenetic analyses (6,16,27,29) of the genus Rymovirus used CP or partial NIb sequences to reconstruct evolutionary history. In these studies, AgMV and HoMV formed a clade with RGMV, whereas WSMV and BrSMV clustered in a separate clade.…”

Section: Discussionmentioning

confidence: 99%

“…Mite-transmitted species of the family Potyviridae include ryegrass mosaic virus (RGMV [29]), wheat streak mosaic virus (11,22), brome streak mosaic virus (BrSMV [17]), and Agropyron mosaic virus (AgMV [26]). The genus Rymovirus (the type species is RGMV) currently includes these four mitetransmitted species plus Hordeum mosaic virus (HoMV [26]), for which a vector has yet to be identified, and several other virus species that have been partially characterized (19,36).…”

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confidence: 99%

“…The genus Rymovirus (the type species is RGMV) currently includes these four mitetransmitted species plus Hordeum mosaic virus (HoMV [26]), for which a vector has yet to be identified, and several other virus species that have been partially characterized (19,36). Recently, based on analysis of coat protein (CP) and, in some cases, partial NIb sequences (6,16,27,29), it has been suggested that the genus Rymovirus is not monophyletic and should be split into at least two genera in which RGMV, HoMV, and AgMV remain within the genus Rymovirus, whereas WSMV and BrSMV constitute a new genus within the family Potyviridae (27). However, in previous efforts, <25% of the viral genomes were analyzed.…”

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confidence: 99%

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Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Stenger¹,

Hall²,

Choi³

et al. 1998

Phytopathology®

124

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The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5′ leader and 149-nt 3′-untranslated region and is polyadenylated at the 3′ end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5′-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3′). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is the type species. Phylogenetic analyses were conducted with complete polyprotein or NIb protein sequences of 11 members of the family Potyviridae, including viruses of monocots or dicots and viruses transmitted by aphids, whiteflies, and mites. WSMV and the monocot-infecting, mite-transmitted brome streak mosaic virus (BrSMV) are sister taxa and share a most recent common ancestor with the whitefly-transmitted sweet potato mild mottle virus, the type species of the proposed genus “Ipomovirus.” In contrast, RGMV shares a most recent common ancestor with aphid-transmitted species of the genus Potyvirus. These results indicate that WSMV and BrSMV should be classified within a new genus of the family Potyviridae and should not be considered species of the genus Rymovirus.

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“…However, coat protein sequence data from New Zealand isolates (unpubl. data) and recently published 3' sequence information from a Danish isolate of RgMV, suggest that mite-transmitted RgMV is more closely related to viruses of the aphid-transmitted potyvirus genus (Schubert et al 1995). This assertion is supported by the serological reaction of RgMV to a number of general potyvirus antisera including PTY1 (Richter et al 1995).…”

Section: Introductionmentioning

confidence: 83%

Distribution of ryegrass mosaic virus in New Zealand perennial ryegrass pastures as determined by ELISA and RT‐PCR

Webster

Forster

Sinclair

et al. 1996

New Zealand Journal of Agricultural Research

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Ryegrass mosaic virus (RgMV) is a mite-transmitted member of the Potyviridae that can seriously affect yield and persistence of ryegrass in pastures. To determine the incidence and distribution of RgMV in New Zealand, perennial ryegrass pastures were surveyed by indirect ELISA. RgMV was detected in all seven mature ( 4 years) North Island pastures and 60% of 20 mature South Island pastures, indicating that RgMV is established throughout New Zealand. A reverse transcriptasepolymerase chain reaction (RT-PCR) assay was developed to confirm the identity of RgMV. This assay was 200 times more sensitive than the indirect ELISA. A possible correlation between numbers of the eriophyid mites and the incidence of RgMV was observed in South Island pastures. Our results suggest that mite transmission may be more significant than ryegrass cultivar, pasture age, endophyte infection, or mechanical transmission in defining the incidence and distribution of RgMV. Options for the control of RgMV in New Zealand pastures are discussed.

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Sequence of the 3'-part of the RNA of ryegrass mosaic virus, a potyvirus

Cited by 10 publications

References 7 publications

Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane

Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Distribution of ryegrass mosaic virus in New Zealand perennial ryegrass pastures as determined by ELISA and RT‐PCR

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