Sequence of the 3'-terminal region of turnip mosaic virus RNA and the capsid protein gene

The complete nucleotide sequence of turnip mosaic potyvirus RNA

“…The high AU content of this 3'-NCR (61.3%) is comparable to that of the 5'-NCR (70~), but no sequence homology was observed. The 3'-NCR displayed 93.3~ identity with that of a Chinese strain of TuMV (Kong et al, 1990), but did not agree with data reported by Tremblay et al (1990). We believe that a cloning artefact was introduced during the previous work.…”

“…A seven amino acid block is sufficient to define the cleavage site recognized by NIa-Pro (Dougherty et aL, 1988) and it has been suggested that differential proteolysis of these sites may regulate potyviral gene expression . The amino acid sequence surrounding the cleavage site between TuMV NIb and CP has been determined previously (Tremblay et al, 1990) and is similar to the NIa-Pro consensus cleavage site of other potyviruses (reviewed in Riechmann et aL, 1992). Based on these consensus NIa-Pro recognition cleavage sites and on the NIb-CP cleavage site sequence, we looked for all potential NIa-Pro cleavage sites in the TuMV amino acid sequence.…”

Characterization of Potyviruses from Tulip and Lily which Cause Flower-Breaking

“…This substitution does not change which amino acid is encoded by the triplet. The sequences were then compared with other known potyvirus sequences, revealing that the sequences of TTBV and TuMV (Tremblay et al, 1990) are almost identical. Both viruses have 14 different nucleotide residues and only one different amino acid residue in this region of the potyvirus genome (Fig.…”

“…3. Of the tulip-breaking viruses, only the distance between TTBV and TuMV appears at the peak representing strains of the same virus, suggesting that only TTBV and TuMV are strain-related and that the other tulip-breaking viruses should be (Frenkel et al, 1991); bean yellow mosaic virus (BYMV; ; SCMV ; Johnsongrass mosaic virus (JGMV)-JG (Shukla et al,i987); potato virus Y (PVY)-10, PVY-18 and PVY-43 (Shukla & Ward, 1988); PVY-D (Shukla, 1986); PVY (Robaglia et al, 1989); pepper mottle virus (PeMV; Dougherty et al, 1985); passionfruit woodiness virus (PWV)-TB, PWV-S and PWV-M (Shukla & Ward, 1988); soybean mosaic virus (SMV)-N (Eggenberger et al, 1989); tobacco etch virus (TEV)-NAT and TEV-HAT ; SMV-V (Gunzyuzlu et al, 1987); plum pox virus (PPV)-NAT and PPV-AT (Maiss et al, 1988(Maiss et al, , 1989PPV-D (Ravelonandro et al, 1988); Gloriosa isolate and LiMV (Langeveld et al, 1991); papaya ringspot virus (PRV)-W (Quemada et al, 1990a); watermelon mosaic virus (WMV) II and zucchini yellow mosaic virus (ZYMV;Quemada et al, 1990b); TuMV (Tremblay et al, 1990). considered distinct potyviruses.…”

Potyvirus serology, sequences and biology

“…The sequences used for the analyses presented in this paper were from the following sources: the complete sequences of tobacco etch virus (TEV) [1]; tobacco vein mottling virus (TVMV) [8]; potato virus Y, N-strain (PVY-NF [42]; and plum pox virus strain NAT (PPV-NAT) [36] and coat-protein sequences of bean yellow mosaic virus strains GDD (BYMV-GDD) [18], PVC (BYMV-PVC) [5], and CS (BYMV-CS) [52]; clover yellow vein virus strain 30 (CIYVV-30) [57]; pea seed-borne mosaic virus (PSbMV) [54]; soybean mosaic virus (SbMV) isolates Gl (SbMV-GI) [46], N (SbMV-N) [11], G2 and G7 (SbMV-G2 and SbMV-G7) [27]; watermelon mosaic virus 2 (WMV) Australian isolate (WMV-2-Aus) [59], and American isolate (WMV-2-US) [39]; papaya ringspot virus strains P and W (PRSV-P, and PRSV-W) [38]; zucchini yellow mosaic virus (ZYMV) isolates from France (ZYMV-F) [39], Israel (ZYMV-I) [12], and Connecticut (ZYMV-CT) [15]; passionfruit woodiness virus (PWV) strains TB, S, and M (PWV-TB, PWV-S and PWV-M) [481; turnip mosaic virus (TuMV) [55]; TEV-NAT [2]; pepper mottle virus (PepMoV) [9]; PVY strains D (PVY-D) [47], 0 (PVY-O) [6] and an isolate from Israel (PVY-I) [43]; ornithogalum mosaic virus (OrMV) [7]; sugarcane mosaic virus (SCMV) [13]; maize dwarf mosaic virus B (MDMV-B) [29]; and PPV strains AT (PPV-AT) [36], D (PPV-D) [41] and Rankovik (PPV-R) [34].…”