Sequence of the nucleocapsid protein gene of subgroup A and B avian pneumoviruses

Li, Jing; Ling, Roger; Randhawa, J S; Shaw, K.; Davis, Philip J.; Juhász, Katalin; Pringle, C. R.; Easton, Andrew J.; Cavanagh, David

doi:10.1016/0168-1702(96)01288-9

Cited by 46 publications

(40 citation statements)

References 17 publications

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“…Similarly, the M protein of subtype C has 78% identity with the corresponding proteins of the A and B subtypes (Seal, 1998), less than the identity shared between the M proteins of subtype A and B (89%; Randhawa et al, 1996a). Comparison of the N proteins of subtype A and B viruses has revealed identity of 89% (Li et al, 1996), greater than when these subtypes are compared with subtype C virus (Dar et al, 2001).…”

Section: Genetic Differences Between Apv Subtypesmentioning

confidence: 99%

“…The first APV gene to be cloned and sequenced was that of the fusion (F) protein gene of a subtype A virus (Yu et al, 1991), since when all the other genes of a subtype A virus have been sequenced (Ling et al, 1992;Yu et al, 1992a,b;Ling et al, 1995;Li et al, 1996;Randhawa et al, 1996bRandhawa et al, , 1997. Fewer genes of subtype B virus have been sequenced: G protein (Juhasz & Easton, 1994), M protein (Randhawa et al, 1996a) and N protein (Li et al, 1996).…”

Section: Sequence Data For the Avian Pneumovirusesmentioning

confidence: 99%

“…Fewer genes of subtype B virus have been sequenced: G protein (Juhasz & Easton, 1994), M protein (Randhawa et al, 1996a) and N protein (Li et al, 1996).…”

Section: Sequence Data For the Avian Pneumovirusesmentioning

confidence: 99%

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Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Cook¹,

Cavanagh

2002

Avian Pathology

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The available detection methods for avian pneumoviruses (turkey rhinotracheitis virus; genus Metapneumovirus) in turkeys, domestic fowl and other species are reviewed. The advantages and disadvantages of virus isolation techniques, virus or genome (polymerase chain reaction) detection and serology are discussed. Some of the problems likely to be encountered are considered, including the detection of yet to be discovered subtypes, as are the factors that are likely to influence the outcome of the work.

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Section: Genetic Differences Between Apv Subtypesmentioning

confidence: 99%

Section: Sequence Data For the Avian Pneumovirusesmentioning

confidence: 99%

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Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Cook¹,

Cavanagh

2002

Avian Pathology

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“…AMPV strains have been divided into four subgroups on the basis of antigenicity and sequence diversity. Subgroups A and B are distinct, but closely related (Juhasz & Easton, 1994;Li et al, 1996;Randhawa et al, 1996), whereas subgroup C is more similar to HMPV (Toquin et al, 2003;Yunus et al, 2003). Subgroup B currently predominates in Europe, whereas subgroup C occurs in the USA and in ducks in France.…”

Section: Introductionmentioning

confidence: 99%

Deletion of the SH gene from avian metapneumovirus has a greater impact on virus production and immunogenicity in turkeys than deletion of the G gene or M2-2 open reading frame

Ling

Sinkovic

Toquin

et al. 2008

Journal of General Virology

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Subgroup A avian metapneumoviruses lacking either the SH or G gene or the M2-2 open reading frame were generated by using a reverse-genetics approach. The growth properties of these viruses were studied in vitro and in vivo in their natural host. Deletion of the SH gene alone resulted in the generation of a syncytial-plaque phenotype and this was reversed by the introduction of the SH gene from a subgroup B, but not a subgroup C, virus. Infected turkeys were assessed for antibody production and the presence of viral genomic RNA in tracheal swabs. The virus with a deleted SH gene also showed the greatest impairment of replication both in cell culture and in infected turkeys. This contrasts with the situation with other pneumoviruses in culture and in model animals, where deletion of the SH gene results in little effect upon viral yield and a good antibody response. Replication of the G-and M2-2-deleted viruses was impaired more severely in turkeys than in cell culture, with only some animals showing evidence of virus growth and antibody production. There was no correlation between virus replication and antibody response, suggesting that replication sites other than the trachea may be important for induction of antibody responses.

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“…G glikoproteinine spesifik monoklonal antikorlar ile yapılan nötralizasyon çalışmaları (10,28) ve virus polipeptidlerin gen sekans analizlerine göre APV'ler 4 alt gruba ayrılmaktadır. Bunlar A, B, C ve D alt tipleridir (18,19,20,33).…”

Section: Introductionunclassified

Hindi ve tavuklarda avian pneumovirus infeksiyonlarının indirek floresan antikor testi ve reverse transkriptaz-polimeraz zincir reaksiyonu ile teşhisi

Ica¹

2005

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada Türkiye'de solunum problemi bulunan tavuk ve hindi sürülerinde Avian pneumovirus (APV) infeksiyonun varlığının belirlenmesi ve sonuçların karşılaştırmalı olarak değerlendirilmesi amaçlandı. Çalışmada 50 tavuk ve 20 hindi sürüsünden toplanan nazal turbinat, nazal svab, akciğer ve trakea örnekleri indirek floresan antikor testi (IFAT) ve reverse transkriptaz-polimeraz zincir reaksiyonu (RT-PCR) teknikleri ile incelendi. RT-PCR ile yapılan çalışmalarda, incelenen tavuk sürüle-rinin %46'sında ve hindi kümeslerinin %50'sinde APV genomu belirlendi. IFAT ile tavuk kümeslerinin %38'inde ve hindi kümesle-rinin %35'inde APV antijenlerinin varlığı saptandı. RT-PCR ile incelenen teşhis materyalleri içinde tavuklarda en yüksek pozitiflik %50 ile nazal turbinatta ve %44.1 ile nazal svabta, hindilerde ise %57.1 ile nazal svabta ve %33.3 ile nazal turbinatta belirlendi. IFAT ile en yüksek pozitiflik aynı materyallerde fakat daha düşük oranlarda tespit edildi. Her iki teknikte de, nazal svab ve nazal turbinatlardan alınan materyallerin akciğer ve trakea örneklerine göre daha uygun olduğu saptandı. APV tanısında RT-PCR'ın IFAT'a göre daha duyarlı olduğu, kullanılan organın ve infeksiyon döneminin sonuçlara etki edebileceği kanısına varıldı.Anahtar sözcükler: Avian pneumovirus, hindi, IFAT, RT-PCR, tavuk. Detection of avian pneumovirus infection in chickens and turkeys by indirect fluorescent antibody test and reverse transcriptase-polymerase chain reactionSummary: The aim of this study was to detect the avian pneumovirus (APV) infection in chicken and turkey flocks with respiratory problems in Turkey. Nasal turbinate, nasal swab, lung and trachea samples collected from 50 chicken and 20 turkey flocks were examined by indirect fluorescent antibody test (IFAT) and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques in the study. APV genome was detected in 46% of chicken flocks and and 50% of turkey flocks by RT-PCR. In IFAT, APV antigens was determined in 38% of chicken flocks and 35% of turkey flocks. On the basis of diagnostic materials used in RT-PCR were considered, the highest positivities were detected in nasal turbinates (50%) and nasal swabs (44.1%) of chickens; and in nazal swabs (57.1%) and nasal turbinates (33.3%) of turkeys. IFAT detected the highest positivity for APV infection in same materials with relatively lower percentages. In both techniques, nasal turbinates and nasal swabs were considered to be superior to the lung and tracheal samples for diagnosis of APV. In conclusion, it was suggested that RT-PCR was more sensitive than IFAT for the detection of APV, but test results might be affected by the test material and the time of infection.

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Sequence of the nucleocapsid protein gene of subgroup A and B avian pneumoviruses

Cited by 46 publications

References 17 publications

Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Deletion of the SH gene from avian metapneumovirus has a greater impact on virus production and immunogenicity in turkeys than deletion of the G gene or M2-2 open reading frame

Hindi ve tavuklarda avian pneumovirus infeksiyonlarının indirek floresan antikor testi ve reverse transkriptaz-polimeraz zincir reaksiyonu ile teşhisi

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