Sequence of the OXA2 β‐lactamase: comparison with other penicillin‐reactive enzymes

Dale, Jeremy W.; Godwin, D.; Mossakowska, Danuta E.; Stephenson, Pauline; Wall, Sue

doi:10.1016/0014-5793(85)80989-3

Cited by 110 publications

(60 citation statements)

References 24 publications

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“…Sequence analysis confirmed the presence of classical bla PER-1 and bla OXA-2 in all these isolates, with sequences identical to those described originally (10,37). The bla OXA-10 -type genes of the ST235 isolates specified a novel ␤-lactamase variant, now designated OXA-74 (http: //www.lahey.org/studies/webt.htm).…”

Section: Pfge and Mlst Pfge Revealed Three Separate Clusters Of Isolsupporting

confidence: 60%

Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum β-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex

et al. 2007

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Forty-one Pseudomonas aeruginosa isolates with extended-spectrum ␤-lactamases (ESBLs) from a hospital in Warsaw, Poland, were analyzed. Thirty-seven isolates from several wards were collected over 9 months in 2003 and 2004. The isolates were recovered from patients with multiple types of infections, mostly respiratory tract and postoperative wound infections. All 41 isolates produced the PER-1 ESBL, originally observed in Turkey but recently also identified in several countries in Europe and the Far East. The bla PER-1 gene resided within the Tn1213 composite transposon, which was chromosomally located. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) revealed the presence of three separate clones among the isolates. Two of these, corresponding to sequence types (STs) ST244 and ST235, were responsible for parallel outbreaks. Apart from PER-1, all the isolates produced OXA-2 oxacillinase. ST235 isolates additionally expressed a novel enzyme, OXA-74, differing by one amino acid from the OXA-17 ESBL identified originally in PER-1-and OXA-2-positive P. aeruginosa isolates from Ankara, Turkey, in 1992. These earlier Ankara isolates with PER-1, OXA-2, and OXA-17 were also classified into ST235, which is a single-locus variant of two other STs, ST227 and ST230. ST227, ST230, and ST235 all correspond to the recently described clonal complex BG11, which seems to be internationally distributed, having spread in Turkey, Greece, Italy, Hungary, Poland, Sweden, and much of Russia. It is associated with various ␤-lactamases, including PER-1 and VIM metalloenzymes. This work further demonstrates the value of MLST of P. aeruginosa.

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Section: Pfge and Mlst Pfge Revealed Three Separate Clusters Of Isolsupporting

confidence: 60%

Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum β-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex

et al. 2007

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“…1987, A-130, p. 22). Probes for TEM-1 and OXA-2 are intragenic (4,31). An intragenic probe was also developed for the PSE-2 bla gene based on the recent determination of its DNA sequence (7).…”

Section: Methodsmentioning

confidence: 99%

Detection of plasmid-mediated beta-lactamases with DNA probes

Huovinen

Jacoby

1988

Antimicrob Agents Chemother

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P-Lactamase identffication by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical isolates making a variety of enzyme types. All strains producing a probe-type enzyme gave a positive hybridization reaction. Cross-hybridization was observed between TEM-1 and TEM-2 or TLE-1, between SHV-1 and SHV-2, between OXA-1 and OXA-4, between OXA-2 and OXA-3 (weak), between PSE-2 and OXA-6 or OXA-5 (weak), and among PSE-1, PSE-4, and CARB-3. With allowance for such cross-hybridization, only six strains gave false-positive reactions, and the procedure was 99% specific.Biochemical studies have established that plasmids in gram-negative bacteria determine a variety of P-lactamase types. The enzymes can be differentiated by substrate profile, reactions with inhibitors, molecular weight, and particularly isoelectric point (pl) into at least 25 kinds (16,19,32). Some, such as TEM-1 and SHV-1, have broad-spectrum but relatively unspecialized activity. As their name implies, the OXA set hydrolyzes oxacillin and related P-lactams with particular efficiency, whereas CARB enzymes, also given PSE designations because they were first found in Pseudomonas aeruginosa, are notably effective in carbenicillin hydrolysis. Distinguishing between the enzymes is of value for epidemiological studies where the presence of a less common P-lactamase type may allow a particular strain or plasmid to be traced (20). The distribution of P-lactamase types in different organisms may also help elucidate where these enzymes originated and how they have spread in response to the use and elaboration of P-lactam antibiotics.Studies relying on isoelectric focusing have demonstrated TEM-1 as the predominant P-lactamase in ampicillin-resistant Escherichia coli and Salmonella spp., whereas SHV-1 is preponderant in Klebsiella spp. (29). In P. aeruginosa carbenicillin or ticarcillin resistance due to ,-lactamase production is the result mainly of PSE-1 or PSE-4 (24, 33).If P-lactamase genes are as diverse as the enzymes they encode, it should be possible to develop DNA probes for P-lactamase identification that would allow more rapid studies with large numbers of strains. DNA fragments suitable as probes for TEM-1 (3, 12, 22), OXA-1 (12, 23), OXA-2 (2), and PSE-1 (12) have been described and have been studied for specificity by utilizing purified plasmid DNA. The purpose of this investigation was to apply such probes to clinical isolates to evaluate their sensitivity and specificity. MATERIALS AND METHODSBacterial strains. strains were demonstrated to grow on plates containing 25 ,ug of ampicillin per ml and to produce ,-lactamase as determined by the nitrocefin test (21). Because no probetype P-lactamases were detected among P. aeruginosa isolates from this source, 20 P. aeruginosa clinical isolates with known plasmid-mediated P-lactamases collected by A. Philippon in France before 1981 were also included in hybridization studies.Analytical isoelectric focusin...

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“…It is difficult to explain the formation of these elements by the same mechanisms as class I compound elements (Schmitt et al, 1985). DNA sequencing (Zolg and Hanggi, 1981;Swift et al, 1981;Dale et al, 1985;Hollingshead and Vapnek, 1985;Tait et al, 1985;Cameron et al, 1986;Hall and Vockler, 1987 Tenover et al, 1988) showed that new Abr genes are inserted in either one of two specific DNA sites, which act as recombination hot-spots (RHSs) and are located at both ends of the aadA gene. We determined that Tn2J codes for a site-specific integration system which may allow the acquisition of new Abr genes .…”

Section: Introductionmentioning

confidence: 99%

Genetic elements involved in Tn21 site-specific integration, a novel mechanism for the dissemination of antibiotic resistance genes.

1990

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Transposon Tn2l codes for a site-specific integration system, which is probably a novel recombination mechanism, responsible for the acquisition of resistance genes in this widespread family of transposons. Using insertion and deletion mutagenesis we have identified the genetic loci of the various recombination hot-spots (RHS) and of the gene product (the integrase) that catalyses the reaction. The site of recombination has been localized in two of the RHSs to the DNA sequence GTTAG, which is present at the 3' terminm of a loosely conserved palindromic sequence of -59 bp. This 59 bp sequence, which flanks the inserted genes in a number of naturally occurring transposons, is the only element required in cis for the recombination reaction.

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Sequence of the OXA2 β‐lactamase: comparison with other penicillin‐reactive enzymes

Cited by 110 publications

References 24 publications

Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum β-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex

Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum β-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex

Detection of plasmid-mediated beta-lactamases with DNA probes

Genetic elements involved in Tn21 site-specific integration, a novel mechanism for the dissemination of antibiotic resistance genes.

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