Sequence of the S9-RNase cDNA and PCR-RFLP system for discriminating S1- to S9-allele in Japanese pear

Takasaki, Takeshi; Okada, Kazuma; Castillo, Carlos; Moriya, Yuki; Saito, Toshihiro; Sawamura, Yutaka; Norioka, Naoko; Norioka, Shigemi; Nakanishi, Tetsu

doi:10.1023/b:euph.0000014907.50575.d0

Cited by 64 publications

(52 citation statements)

References 23 publications

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“…Since almost all cultivars of Japanese pear exhibit SI, S-genotype assignment is essential for stable fruit production and efficient breeding. Studies on the pistil S gene have led to the establishment of the CAPS system for S-genotyping based on sequence polymorphisms of the S-RNase allele (Castillo et al, 2002;Ishimizu et al, 1999;Takasaki et al, 2004). Similarly, studies on the pollen S gene have led to the development of the CAPS/dCAPS system for S-genotyping based on sequence polymorphisms of the PpSFBB -γ allele .…”

Section: Discussionmentioning

confidence: 99%

“…2015. The new S-RNase was designated as S 9 -RNase, and the S-genotypes of 'Shinkou' and 'Shinsei' were identified as S 4 S 9 . Takasaki et al (2004) isolated the full-length cDNA encoding the S 9 -RNase from pistils of 'Shinkou' and 'Shinsei', and established the CAPS (S 1 to S 9 ) system for discriminating S 1 -to S 9 -haplotypes. In this system, the nine S-RNase fragments are amplified from genomic DNA by PCR using the primer pair "FTQQYQ" and "anti-(I/T)IWPNV" [a mix of the primer "anti-IIWPNV" and an S 9 -RNase-specific primer "anti-TIWPNV" (5'-ACGTTTGGCCAAATAGTT-3')] ( Fig.…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

“…Using this system, the S-genotypes of 'Niitaka', 'Nansui', 'Nangetsu', 'Amanogawa', and 'Ishiiwase' were identified as S 3 S 9 , S 4 S 9 , S 4 S 9 , S 1 S 9 , and S 3 S 9 , respectively Takasaki et al, 2004). Moreover, these CAPS systems were used to reconsider the S-genotypes of five cultivars for which there was some uncertainty.…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

“…To date, the CAPS systems have been used to identify the S-genotypes of 77 Japanese pear cultivars (Castillo et al, 2001a(Castillo et al, , b, 2002Ishimizu et al, 1999;Okada et al, 2004;Sawamura et al, 2002bSawamura et al, , 2008Takasaki et al, 2004) (Table 2); 20 cultivars were identified as S 4 S 5 and 9 cultivars as S 3 S 4 . This result indicates that there are many cross-incompatible combinations among Japanese pear cultivars.…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

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DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (Pyrus pyrifolia Nakai)

Okada

2015

Hortic J

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Japanese pear (Pyrus pyrifolia Nakai) is an important Rosaceous fruit tree in Japan. This species exhibits gametophytic self-incompatibility (GSI), which is controlled by a single S-locus with multiple S-haplotypes. Although GSI is a genetic mechanism to prevent inbreeding and promote outcrossing to maintain genetic diversity, it can be problematic in fruit trees because it causes unstable fruit set. Therefore, orchardists interplant Japanese pear with other lines as pollinizers or conduct artificial pollination to ensure fruit set. To achieve stable fruit production and to reduce or eliminate the need for artificial pollination, research on the GSI of Japanese pear has been conducted by pollination experiments and by characterization of selfcompatible (SC) mutants. Additionally, breeding programs have progressed to produce SC cultivars with high fruit quality. Recently, molecular analyses of GSI and SC mutants in Japanese pear have provided new information that is relevant to the stable fruit production and efficient breeding of Japanese pear. This review focuses on studies of the GSI of Japanese pear, and especially on the recent development of DNA markers for S-genotyping and marker-assisted selection of SC trees. In addition, the candidate genes controlling pollen S specificity and a model of the molecular mechanism of GSI in Japanese pear are described.

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Section: Discussionmentioning

confidence: 99%

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

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DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (Pyrus pyrifolia Nakai)

Okada

2015

Hortic J

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“…The S-RNase genotypes of Japanese pear cultivars were determined by PCR-RFLP according to the protocol of Ishimizu et al (1999) and Takasaki et al (2004). Briefly, genomic DNA was extracted from fresh young leaves using a modified CTAB protocol (Yamamoto et al 2000).…”

Section: S-rnase Typing Of Plant Materials and Developed Progenymentioning

confidence: 99%

A Self-compatible Pollen-part Mutant of Japanese Pear Produced by Crossing ‘Kosui’ with Pollen from Gamma-irradiated ‘Kosui’

Sawamura

Mase

Takada

et al. 2013

J. Japan. Soc. Hort. Sci.

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The self-incompatible (SI) Japanese pear cultivar 'Kosui' was pollinated with pollen collected from a chronically gamma-irradiated 'Kosui' tree, and a progeny was obtained. This progeny resulted in the identification of selfcompatible (SC) breeding selection, designated 415-1, which showed 74.4% fruit set in a self-pollination test. PCR-based genetic analysis revealed that 415-1 has S-RNase genotype S 4 S 5 , which is the same as that of the parent, 'Kosui'. Pollination trials were used to investigate whether 415-1 harbors a stylar-part mutation or a pollen-part mutation in its SI locus. When 415-1 was pollinated with pollen from cultivars of the same genotype ('Syuugyoku' and 'Oushuu'), no seed-containing fruit were set, indicating that 415-1 contains functional S 4 -and S 5 -RNase alleles. On the other hand, when 'Syuugyoku' and 'Oushuu' were pollinated with pollen from 415-1, fruit-bearing seeds were produced; therefore, we conclude that 415-1 carries a pollen-part mutation at the SI locus. This new self-compatible breeding selection will be useful for the development of new Japanese pear cultivars with SC.

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Pear Genomics

Yamamoto

Chevreau

2009

Genetics and Genomics of Rosaceae

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Sequence of the S₉-RNase cDNA and PCR-RFLP system for discriminating S₁- to S₉-allele in Japanese pear

Cited by 64 publications

References 23 publications

DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (<i>Pyrus pyrifolia</i> Nakai)

DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (<i>Pyrus pyrifolia</i> Nakai)

A Self-compatible Pollen-part Mutant of Japanese Pear Produced by Crossing ‘Kosui’ with Pollen from Gamma-irradiated ‘Kosui’

Pear Genomics

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