Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes

Gerlach, W. L.; Dyer, Tristan A.

doi:10.1093/nar/8.21.4851

Cited by 487 publications

(272 citation statements)

References 29 publications

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“…The DNA probes used in these analyses were pTa71, a 9-kb fragment from T. aestivum containing the 18S ribosomal DNA (rDNA) sequence including the intergenic spacers (Gerlach and Bedbrook 1979), and pTa794 (a 410-bp fragment also from wheat containing the 5S rDNAgene repeat unit) (Gerlach and Dyer 1980). Genomic Fig.…”

Section: Fish Gish Probes and Labellingmentioning

confidence: 99%

Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv.

et al. 2011

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This paper reports the glutenin diversity of a collection of 23 wild populations of the grass Brachypodium distachyon collected in the Mediterranean and southern areas of the Iberian Peninsula. The plant material studied included the three different cytotypes of this species: 2n = 10, 2n = 20 and 2n = 30. A specific method of extraction was used to isolate the glutenin subunits from the caryopsis. Separation by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed them to correspond to wheat low-molecular-weight glutenin subunits (LMW-GS). Twenty-two LMW-GS-like were identified that showed great diversity within and between populations. All the populations investigated were polymorphic for the endosperm proteins studied. The 2n = 30 forms had the largest number of subunits; these were also more diverse than those of the 2n = 10 or 2n = 20 forms. The 2n = 10 forms, the most common in the higher, interior areas of the Iberian Peninsula, showed the smallest subunit variation. In fact, negative correlations were found between subunit diversity and altitude and longitude. In contrast, a positive correlation was detected with the annual average temperature and the indices of thermicity and Mediterraneity. The similarity between the populations was estimated using the Sorensen-Dice coefficient, calculated on the basis of the presence/absence of the 22 LMW-GS proteins. The similarity indices were used to produce a dendrogram using the unweighted pair-group method with arithmetic means (UPGMA). This method produced three main groups corresponding to the three cytotypes. An analysis was made of the possible correlation between eco-geographic and climatic factors and the gene diversity and polymorphism shown in the populations. The diversity correlated positively with the number of chromosomes (2n), annual mean temperature, index of thermicity and index of Mediterraneity. In contrast, diversity correlated negatively with altitude, longitude and index of precipitation during the summer. The number of chromosomes correlated negatively with altitude, longitude and precipitation during summer and positively with all the other climatic indices. These results are coherent with the fact that diploid forms were more common in areas of the interior and at higher altitude, where the climate is more extreme.

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Section: Fish Gish Probes and Labellingmentioning

confidence: 99%

Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv.

et al. 2011

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“…The availability of mapped YACs allowed us to sequence 5S units from identified 5S blocks. Gerlach and Dyer (1980) suggested that only the short 5S repeat unit was transcriptionally active in wheat, with the different repeats (short and long) organized in separate clusters, each cluster on a separate chromosome (Dvorak et al 1989). In the case of the absence of transcription of some Arabidopsis 5S blocks, what mechanisms could have led to this extinction?…”

Section: Genome Research 139mentioning

confidence: 99%

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Cloix¹,

Tutois²,

Yukawa³

et al. 2001

Genome Res.

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One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5Ј-and 3Ј-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.

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“…1. In addition, two ribosomal probes pTa71(18S-5.8S-25S rDNA; Gerlach & Bedbrook, 1979) and pTa794 (5S rDNA; Gerlach & Dyer, 1980) were used in some experiments to study the chromosomes in more detail.…”

Section: Dna Probes and Labellingmentioning

confidence: 99%

Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes

et al. 1999

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A distant hybrid between two diploid species (2n = 2x = 16), Alstroemeria aurea and A. inodora, was investigated for homoeologous chromosome pairing, crossability with A. inodora and chromosome transmission to its BC 1 offspring. Fluorescence in situ hybridization (FISH) with two species-specific probes, A001-I (A. aurea specific) and D32-13 (A. inodora specific), was used to analyse chromosome pairing in the hybrid and the genome constitution of its BC 1 progeny plants. High frequencies of associated chromosomes were observed in both genotypes of the F 1 hybrid, A1P2-2 and A1P4. In the former, both univalents and bivalents were found at metaphase I, whereas the latter plant also showed tri-and quadrivalents. Based on the hybridization sites of DNA probes on the chromosomes of both parental species, it was established that hybrid A1P4 contains a reciprocal translocation between the short arm of chromosome 1 and the long arm of chromosome 8 of A. inodora. Despite regular homoeologous chromosome pairing in 30% of the pollen mother cells, both hybrids were highly sterile. They were backcrossed reciprocally with one of the parental species, A. inodora. Two days after pollination, embryo rescue was applied and, eventually, six BC 1 progeny plants were obtained. Among these, two were aneuploids (2n = 2xǹ1 = 17) and four were triploids (2n = 3x = 24). The aneuploid plants had originated when the interspecific hybrid was used as a female parent, indicating that n eggs were functional in the hybrid. In addition, 2n gametes were also functional in the hybrid, resulting in the four triploid BC 1 plants. Of these four plants, three had received 2n pollen grains from the hybrid and one a 2n egg. Using FISH, homoeologous crossing over between the chromosomes of the two parental species in the hybrid was clearly detected in all BC 1 plants. The relevance of these results for the process of introgression and the origin of n and 2n gametes are discussed.

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Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes

Cited by 487 publications

References 29 publications

Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv.

Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv.

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes

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