Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

“…Since SRAP was reported in 2001 by Li and Quiros, it has emerged as a new and useful PCR marker technique for germplasm characterization, cultivar identification, molecular mapping and gene cloning in crop plants in recent years (Aneja et al 2012). We found that these markers could detect 4.65 alleles per locus making SRAP analysis very useful in assessing mungbean diversity as these were highly polymorphic (100 %).…”

“…The merits of SRAP over other marker systems are its simplicity and reliability, reasonable throughput rate, targets ORFs in genome, numerous co-dominant and clear high-intensity bands rarely overlapping, not crop-specific, easy isolation of bands for sequencing, cost-effective. Any of the forward primers can be combined with any of the reverse primers, so many primer combinations are possible, reducing the cost of PCR, very important in the developing countries (Aneja et al 2012). …”

“…This SRAP marker can be validated in other micronutrient dense genotypes by screening a large number of germplasm. Further it can be converted into a SCAR marker for its use in markerassisted selection in breeding iron dense mungbean genotypes (Aneja 2010). SRAP emerges as highly productive technique in crops where genome sequence is not available particularly in case of under-privileged crops such as mungbean for marker assisted breeding and related applications.…”

“…There is large scope for saturating the existing linkage map of mungbean. SRAP, a highly polymorphic marker system offers great potential for improving the existing linkage map and association mapping as these markers have been used in different crops for these applications (Aneja et al 2012).…”

New genetic maps for globe artichoke and wild cardoon and their alignment with an SSR-based consensus map

“…The choice of SRAP PCs reflected prior experience ) and, as SRAP genotyping generated most of the markers applied, these loci constituted the major backbone of the two maps. Since its development some ten years ago, SRAP genotyping has been deployed in a range of plant species for estimating levels of genetic diversity Esposito et al 2007;Aneja et al 2012), gene tagging (Martin et al 2008;Zhang et al 2011) and map construction (Lin et al 2003;Sun et al 2007;Xue et al 2010). However, here we report its first usage for a genetic mapping in C. cardunculus.…”